Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
Complement C5a Antibody (MA5-23910) in Neu
Neutralization of Complement C5a in dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line. Samples were incubated in Complement C5a monoclonal antibody (Product # MA5-23910). Recombinant Mouse Complement Component C5a induces N‚acetyl- β-D-glucosaminidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N‚Acetyl- β-D-Glucosaminidase Release elicited by Recombinant Mouse Complement Component C5a (50 ng/mL) is neutralized (green line... View More
Product Details
MA5-23910
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2A
Class
Monoclonal
Type
Antibody
Clone
295108
Immunogen
E. coli-derived recombinant mouse Complement Component C5a Asn679-Arg755
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609685
Product Specific Information
In Western blots, this antibody does not cross-react with recombinant mouse Complement Component C3d, recombinant human (rh) Complement Component C3a, or rhComplement Component C5a.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: C4B1; C4B12; C4B2; C4B3; C4B5; C4F; CH; CO4; Complement C5; CPAMD3; DADB-112B14.11; FLJ17816; FLJ17822; FLJ60561; Hemolytic complement; MGC142298; MGC164979
Gene Aliases: C5; C5a; Hc; He
UniProt ID: (Mouse) P06684
Entrez Gene ID: (Mouse) 15139
in utero embryonic development
immune system process
inflammatory response
complement activation, alternative pathway
complement activation, classical pathway
positive regulation of vascular endothelial growth factor production
negative regulation of macrophage chemotaxis
cytolysis
innate immune response
positive regulation of angiogenesis
positive regulation of chemokine secretion
mitophagy in response to mitochondrial depolarization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support