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Connexin 40 Antibody (OSC00189W-100UL) in IHC (P)
IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40: OSC00189W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Ha... View More
Product Details
OSC00189W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from mouse Connexin-40 conjugated to an blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide shares 95% identity with rat sequence.
Specificity of this antibody: Connexin-40.
Target Information
Connexin 40 (Cx40), belongs to the connexin family of gap junction proteins, which are expressed in pancreas, kidney, skeletal muscle, liver, placenta and heart. Connexin 40 is highly expressed in vascular endothelial cells, and the mammalian heart (specifically in the atrial myocardium, and the His-Purkinje conduction system). At least three other connexins are expressed in the heart, including Cx37 (endocardium), Cx43 (atrial and ventricular myocardium, and distal parts of the conduction system) and Cx45 (throughout the heart). Cx40 and Cx43 frequently co-localize in cells of the cardiovascular system and participate in the formation of heterotypic gap junction channels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Connexin-40; Cx40; Gap junction alpha-5 protein; gap junction membrane channel protein alpha 5
Gene Aliases: 5730555N10Rik; Cnx40; Cx40; Cxn-40; Gja-5; Gja5
UniProt ID: (Mouse) Q01231
Entrez Gene ID: (Mouse) 14613
skeletal system development
blood vessel development
renal system process involved in regulation of systemic arterial blood pressure
regulation of systemic arterial blood pressure
negative regulation of glomerular filtration
outflow tract morphogenesis
cardiac conduction system development
mitral valve development
pulmonary valve formation
ventricular septum development
atrial septum development
septum primum development
atrial ventricular junction remodeling
potassium ion transport
cell communication
cell communication by chemical coupling
cell communication by electrical coupling
positive regulation of cell communication by chemical coupling
gap junction assembly
embryonic limb morphogenesis
embryonic heart tube development
foramen ovale closure
regulation of vasodilation
negative regulation of blood pressure
positive regulation of vasoconstriction
positive regulation of vasodilation
artery morphogenesis
protein oligomerization
transmembrane transport
regulation of cardiac muscle contraction
regulation of ventricular cardiac muscle cell membrane repolarization
regulation of atrial cardiac muscle cell membrane depolarization
regulation of ventricular cardiac muscle cell membrane depolarization
ventricular septum morphogenesis
atrial septum morphogenesis
cardiac conduction
ventricular cardiac muscle cell action potential
SA node cell action potential
AV node cell to bundle of His cell communication by electrical coupling
bundle of His cell to Purkinje myocyte communication by electrical coupling
cell communication by electrical coupling involved in cardiac conduction
cell communication involved in cardiac conduction
AV node cell to bundle of His cell communication
regulation of heart rate by cardiac conduction
regulation of AV node cell action potential
regulation of bundle of His cell action potential
regulation of Purkinje myocyte action potential
regulation of atrial cardiac muscle cell action potential
regulation of renin secretion into blood stream
regulation of membrane depolarization during cardiac muscle cell action potential
vasomotion
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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