Invitrogen

Connexin 43 Polyclonal Antibody

View all (41) Connexin 43 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-32778

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL
-
Product Specifications

Species Reactivity

Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic 16 amino acid peptide from C-terminus of human GJA1 / Connexin 43.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2550240

Product Specific Information

Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Rabbit, Pig, Opossum, Guinea pig (100%); Bat, Horse, Chicken, Lizard, Xenopus (94%); Trout, Salmon (88%); Zebrafish, Sea anemone, Nematode (81%).

For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein; gap junction protein connexin 43; gap junction protein, alpha 1, 43 kD (connexin 43); gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); ODD; ODOD; SDTY3; Syndactyly type III; Vascular smooth muscle connexin-43; vascular smooth muscle connexin43

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Gene Aliases: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

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UniProt ID: (Rabbit) Q6TYA7, (Human) P17302, (Bovine) P18246, (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Rabbit) 100008935, (Human) 2697, (Pig) 100518636, (Dog) 403418, (Bovine) 281193, (Mouse) 14609, (Rat) 24392, (Chinese hamster) 100689077, (Guinea pig) 100379273

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Function(s)
signal transducer activity receptor binding gap junction channel activity protein binding ion transmembrane transporter activity SH3 domain binding PDZ domain binding beta-tubulin binding connexin binding gap junction channel activity involved in cardiac conduction electrical coupling scaffold protein binding gap junction channel activity involved in cell communication by electrical coupling tubulin binding gap junction hemi-channel activity beta-catenin binding protein domain specific binding transmembrane transporter activity gap junction
Process(es)
osteoblast differentiation in utero embryonic development neuron migration negative regulation of endothelial cell proliferation heart looping regulation of heart rate epithelial cell maturation lens development in camera-type eye chronic inflammatory response positive regulation of glomerular filtration endothelium development atrial ventricular junction remodeling transport apoptotic process muscle contraction signal transduction positive regulation of cytosolic calcium ion concentration cell-cell signaling heart development adult heart development response to pH response to glucose vascular transport positive regulation of gene expression negative regulation of gene expression cell communication by chemical coupling cell communication by electrical coupling positive regulation of cell communication by chemical coupling ATP transport gap junction assembly negative regulation of cell growth regulation of bone mineralization positive regulation of insulin secretion ion transmembrane transport response to fluid shear stress embryonic digit morphogenesis positive regulation of I-kappaB kinase/NF-kappaB signaling skeletal muscle tissue regeneration response to peptide hormone positive regulation of osteoblast differentiation positive regulation of protein catabolic process positive regulation of striated muscle tissue development positive regulation of vasoconstriction positive regulation of vasodilation regulation of bone remodeling blood vessel morphogenesis neuron projection morphogenesis protein oligomerization regulation of calcium ion transport negative regulation of cardiac muscle cell proliferation milk ejection regulation of ventricular cardiac muscle cell membrane repolarization regulation of atrial cardiac muscle cell membrane depolarization regulation of ventricular cardiac muscle cell membrane depolarization negative regulation of wound healing cellular response to mechanical stimulus atrial cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction negative regulation of DNA biosynthetic process regulation of bicellular tight junction assembly positive regulation of behavioral fear response response to ischemia spermatogenesis response to wounding cellular response to platelet-derived growth factor stimulus regulation of apoptotic process bone remodeling transmembrane transport bone development positive regulation of smooth muscle cell chemotaxis microtubule-based transport establishment of mitotic spindle orientation cell communication regulation of heart contraction negative regulation of cell proliferation negative regulation of gonadotropin secretion cellular protein localization embryonic heart tube development negative regulation of trophoblast cell migration positive regulation of morphogenesis of an epithelium positive regulation of mesodermal cell differentiation positive regulation of stem cell proliferation epicardial cell to mesenchymal cell transition cell-cell junction organization regulation of blood vessel remodeling cardiac conduction establishment of epithelial cell polarity
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