Invitrogen

Connexin 43 Monoclonal Antibody (3D8A5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

50 Published Figures

48 References

View all (41) Connexin 43 antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Connexin 43 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 53

Connexin 43 Antibody (35-5000) in IHC

Double immunofluorescent staining of mouse heart atrium using mouse anti-Connexin 43 monoclonal antibody (Product # 35-5000) (red) and Rabbit anti-Connexin 40 (C-term, Product # 36-4900) (green). Cx43 immunolocalizes to the intercalated discs throughout cardiac tissue, while Cx40 immunolabeling is localized to intercalated discs restricted to the conduction system. Image courtesy of James I. Nagy, PhD, Univ... View More

Connexin 43 Antibody in Western Blot (WB)

Connexin 43 Antibody in Immunocytochemistry (ICC/IF)

Connexin 43 Antibody in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC, IHC (P))

Connexin 43 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Connexin 43 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Connexin 43 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Connexin 43 Antibody in In Situ Hybridization (ISH) (ISH)

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Product Details

35-5000

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 17 publications 17 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

In Situ Hybridization (ISH) (ISH)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3D8A5

Immunogen

Synthetic peptide derived from rat Connexin 43.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533207

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities.

eLife

Mastrototaro G,Carullo P,Zhang J,Scellini B,Piroddi N,Nemska S,Filomena MC,Serio S,Otey CA,Tesi C,Emrich F,Linke WA,Poggesi C,Boncompagni S,Bang ML

35-5000 was used in Western Blot to study the role of Palladin (PALLD)in the heart.

Thu Mar 16 00:00:00 EDT 2023

Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities.

eLife

Mastrototaro G,Carullo P,Zhang J,Scellini B,Piroddi N,Nemska S,Filomena MC,Serio S,Otey CA,Tesi C,Emrich F,Linke WA,Poggesi C,Boncompagni S,Bang ML

35-5000 was used in Western Blot to study the role of Palladin (PALLD)in the heart.

Thu Mar 16 00:00:00 EDT 2023

Cardiac ultrastructure inspired matrix induces advanced metabolic and functional maturation of differentiated human cardiomyocytes.

Cell reports

Afzal J,Liu Y,Du W,Suhail Y,Zong P,Feng J,Ajeti V,Sayyad WA,Nikolaus J,Yankova M,Deymier AC,Yue L

35-5000 was used in Immunocytochemistry to present a method to accelerate hiPSC-CM maturation on a substrate, cardiac mimetic matrix (CMM), mimicking adult human heart matrix ligand chemistry, rigidity, and submicron ultrastructure, which synergistically mature hiPSC-CMs rapidly within 30 days.

Tue Jul 26 00:00:00 EDT 2022

Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNA in situ hybridization multiplexing.

STAR protocols

Pang J,Thomas N,Tsuchiya D,Parmely T,Yan D,Xie T,Wang Y

35-5000 was used in Immunohistochemistry to describe a protocol for preparation of mouse eye sections composed of Hartman's fixation, window technique, microwave-based tissue processing, optimized depigmentation, and antigen retrieval pretreatment.

Fri Dec 17 00:00:00 EST 2021

Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNA in situ hybridization multiplexing.

STAR protocols

Pang J,Thomas N,Tsuchiya D,Parmely T,Yan D,Xie T,Wang Y

Fri Dec 17 00:00:00 EST 2021

Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption.

International journal of molecular sciences

Godinho-Pereira J,Garcia AR,Figueira I,Malhó R,Brito MA

35-5000 was used in Immunocytochemistry to study the components involved in the interaction between breast cancer cells and blood-brain barrier (BBB) endothelial cells underlying BBB alterations and the transendothelial migration of malignant cells.

Wed Jun 30 00:00:00 EDT 2021

NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression.

Cell reports

Pang J,Le L,Zhou Y,Tu R,Hou Q,Tsuchiya D,Thomas N,Wang Y,Yu Z,Alexander R,Thexton M,Lewis B,Corbin T,Durnin M,Li H,Ashery-Padan R,Yan D,Xie T

35-5000 was used in Western Blot, Immunohistochemistry to show that NOTCH signaling in the ciliary body (CB) maintains the vitreous, intraocular pressure, and eye structures by regulating CB morphogenesis, aqueous humor secretion, and vitreous protein expression.

Tue Jan 12 00:00:00 EST 2021

NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression.

Cell reports

Pang J,Le L,Zhou Y,Tu R,Hou Q,Tsuchiya D,Thomas N,Wang Y,Yu Z,Alexander R,Thexton M,Lewis B,Corbin T,Durnin M,Li H,Ashery-Padan R,Yan D,Xie T

Tue Jan 12 00:00:00 EST 2021

Morphological study of a connexin 43-GFP reporter mouse highlights glial heterogeneity, amacrine cells, and olfactory ensheathing cells.

Journal of neuroscience research

Theofilas P,Steinhäuser C,Theis M,Derouiche A

355000 was used in immunohistochemistry to develop a mouse line to examine connexin 43-expressing central nervous system cells and astroglial cell morphology

Wed Nov 01 00:00:00 EDT 2017

Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland.

The European journal of neuroscience

Tsao DD,Wang SG,Lynn BD,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Thu Jun 01 00:00:00 EDT 2017

Mdivi-1 induced acute changes in the angiogenic profile after ischemia-reperfusion injury in female mice.

Physiological reports

Veeranki S,Tyagi SC

35-5000 was used in Western Blotting to determine the effects of mitochondrial division inhibitor 1 on the angiogenic profiles following ischaemia reperfusion injury in mice.

Thu Jun 01 00:00:00 EDT 2017

Effects of Ang Ⅱ perfusion on transmural heterogeneous of Cx43 in acute myocardial ischemia reperfusion.

Asian Pacific journal of tropical medicine

Chen XP,Lu SJ,Huang K,Zhang W,Liu ZW,Zhong JH

35-5000 was used in western blot to study the effects on transmural heterogeneous of Cx42 by Ang II perfusion in acute myocardial ischemia reperfusion

Fri Jan 01 00:00:00 EST 2016

MMP-9- and NMDA receptor-mediated mechanism of diabetic renovascular remodeling and kidney dysfunction: hydrogen sulfide is a key modulator.

Nitric oxide : biology and chemistry

Kundu S,Pushpakumar S,Sen U

35-5000 was used in western blot to test if H2S supplementation reduces MMP-9-induced extracellular matrix remodeling and dysfunction in diabetic kidney.

Thu Apr 30 00:00:00 EDT 2015

G-protein-coupled inward rectifier potassium current contributes to ventricular repolarization.

Cardiovascular research

Liang B,Nissen JD,Laursen M,Wang X,Skibsbye L,Hearing MC,Andersen MN,Rasmussen HB,Wickman K,Grunnet M,Olesen SP,Jespersen T

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2014

G-protein-coupled inward rectifier potassium current contributes to ventricular repolarization.

Cardiovascular research

Liang B,Nissen JD,Laursen M,Wang X,Skibsbye L,Hearing MC,Andersen MN,Rasmussen HB,Wickman K,Grunnet M,Olesen SP,Jespersen T

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2014

G-protein-coupled inward rectifier potassium current contributes to ventricular repolarization.

Cardiovascular research

Liang B,Nissen JD,Laursen M,Wang X,Skibsbye L,Hearing MC,Andersen MN,Rasmussen HB,Wickman K,Grunnet M,Olesen SP,Jespersen T

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Jan 01 00:00:00 EST 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

The European journal of neuroscience

Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Sat Mar 01 00:00:00 EST 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

The European journal of neuroscience

Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Sat Mar 01 00:00:00 EST 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

The European journal of neuroscience

Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Sat Mar 01 00:00:00 EST 2014

AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice.

Genetics

Schimenti KJ,Feuer SK,Griffin LB,Graham NR,Bovet CA,Hartford S,Pendola J,Lessard C,Schimenti JC,Ward JO

35-5000 was used in Immunohistochemistry to identify and support a novel reproductive tissue-specific role for Akap9 in the coordinated regulation of Sertoli cells in the testis.

Sat Jun 01 00:00:00 EDT 2013

Ablation of connexin30 in transgenic mice alters expression patterns of connexin26 and connexin32 in glial cells and leptomeninges.

The European journal of neuroscience

Lynn BD,Tress O,May D,Willecke K,Nagy JI

35-5000 was used in immunohistochemistry (frozen) to study localization of connexins in the brain.

Thu Dec 01 00:00:00 EST 2011

Connexin37 and Connexin43 deficiencies in mice disrupt lymphatic valve development and result in lymphatic disorders including lymphedema and chylothorax.

Developmental biology

Kanady JD,Dellinger MT,Munger SJ,Witte MH,Simon AM

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunohistochemistry (Frozen)

Wed Jun 15 00:00:00 EDT 2011

Connexin37 and Connexin43 deficiencies in mice disrupt lymphatic valve development and result in lymphatic disorders including lymphedema and chylothorax.

Developmental biology

Kanady JD,Dellinger MT,Munger SJ,Witte MH,Simon AM

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunohistochemistry (Frozen)

Wed Jun 15 00:00:00 EDT 2011

Increased coupling and altered glutamate transport currents in astrocytes following kainic-acid-induced status epilepticus.

Neurobiology of disease

Takahashi DK,Vargas JR,Wilcox KS

35-5000 was used in western blot to test if hippocampal astrocytes have altered function soon after kainic acid-induced status epilepticus

Wed Dec 01 00:00:00 EST 2010

Increased coupling and altered glutamate transport currents in astrocytes following kainic-acid-induced status epilepticus.

Neurobiology of disease

Takahashi DK,Vargas JR,Wilcox KS

35-5000 was used in western blot to test if hippocampal astrocytes have altered function soon after kainic acid-induced status epilepticus

Wed Dec 01 00:00:00 EST 2010

Phosphorylation of connexin43 on serine 306 regulates electrical coupling.

Heart rhythm

Procida K,Jørgensen L,Schmitt N,Delmar M,Taffet SM,Holstein-Rathlou NH,Nielsen MS,Braunstein TH

35-5000 was used in immunohistochemistry to discuss connexin 43 phosphorylation

Sun Nov 01 00:00:00 EDT 2009

Phosphorylation of connexin43 on serine 306 regulates electrical coupling.

Heart rhythm

Procida K,Jørgensen L,Schmitt N,Delmar M,Taffet SM,Holstein-Rathlou NH,Nielsen MS,Braunstein TH

35-5000 was used in immunohistochemistry to discuss connexin 43 phosphorylation

Sun Nov 01 00:00:00 EDT 2009

Connexin40 imparts conduction heterogeneity to atrial tissue.

Circulation research

Leaf DE,Feig JE,Vasquez C,Riva PL,Yu C,Lader JM,Kontogeorgis A,Baron EL,Peters NS,Fisher EA,Gutstein DE,Morley GE

35-5000 was used in immunohistochemistry - frozen section to examine the role of Cx40 in atrial impulse propagation

Fri Oct 24 00:00:00 EDT 2008

Connexin 43 hemichannels are permeable to ATP.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kang J,Kang N,Lovatt D,Torres A,Zhao Z,Lin J,Nedergaard M

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Apr 30 00:00:00 EDT 2008

Connexin 43 hemichannels are permeable to ATP.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kang J,Kang N,Lovatt D,Torres A,Zhao Z,Lin J,Nedergaard M

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Apr 30 00:00:00 EDT 2008

Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions.

The European journal of neuroscience

Li X,Penes M,Odermatt B,Willecke K,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Oct 01 00:00:00 EDT 2008

Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions.

The European journal of neuroscience

Li X,Penes M,Odermatt B,Willecke K,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Oct 01 00:00:00 EDT 2008

Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions.

The European journal of neuroscience

Li X,Penes M,Odermatt B,Willecke K,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Oct 01 00:00:00 EDT 2008

Connexin mimetic peptides fail to inhibit vascular conducted calcium responses in renal arterioles.

American journal of physiology. Regulatory, integrative and comparative physiology

Sorensen CM,Salomonsson M,Braunstein TH,Nielsen MS,Holstein-Rathlou NH

35-5000 was used in immunocytochemistry to elucidate the role of various connexins in transmission of conducted vasoconstrictor signals in the preglomerular arterioles

Mon Sep 01 00:00:00 EDT 2008

Connexin-43 upregulation in micrometastases and tumor vasculature and its role in tumor cell attachment to pulmonary endothelium.

BMC medicine

Elzarrad MK,Haroon A,Willecke K,Dobrowolski R,Gillespie MN,Al-Mehdi AB

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunohistochemistry

Tue Jul 22 00:00:00 EDT 2008

Connexin-43 upregulation in micrometastases and tumor vasculature and its role in tumor cell attachment to pulmonary endothelium.

BMC medicine

Elzarrad MK,Haroon A,Willecke K,Dobrowolski R,Gillespie MN,Al-Mehdi AB

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunohistochemistry

Tue Jul 22 00:00:00 EDT 2008

Connexin-43 upregulation in micrometastases and tumor vasculature and its role in tumor cell attachment to pulmonary endothelium.

BMC medicine

Elzarrad MK,Haroon A,Willecke K,Dobrowolski R,Gillespie MN,Al-Mehdi AB

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunohistochemistry

Tue Jul 22 00:00:00 EDT 2008

Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus.

Neuroscience

Rash JE,Olson CO,Davidson KG,Yasumura T,Kamasawa N,Nagy JI

35-5000 was used in immunohistochemistry to determine the abundance and connexin composition of neuronal and glial gap junctions in developing and adult rat and mouse locus coeruleus

Sun Jul 29 00:00:00 EDT 2007

Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus.

Neuroscience

Rash JE,Olson CO,Davidson KG,Yasumura T,Kamasawa N,Nagy JI

35-5000 was used in immunohistochemistry to determine the abundance and connexin composition of neuronal and glial gap junctions in developing and adult rat and mouse locus coeruleus

Sun Jul 29 00:00:00 EDT 2007

Reduction and redistribution of gap and adherens junction proteins after ischemia and reperfusion.

The Annals of thoracic surgery

Tansey EE,Kwaku KF,Hammer PE,Cowan DB,Federman M,Levitsky S,McCully JD

35-5000 was used in western blot to assess the structural changes that occur after myocardial ischemia and reperfusion

Sun Oct 01 00:00:00 EDT 2006

Prolonged labour associated with lower expression of syndecan 3 and connexin 43 in human uterine tissue.

Reproductive biology and endocrinology : RB&E

Cluff AH,Byström B,Klimaviciute A,Dahlqvist C,Cebers G,Malmström A,Ekman-Ordeberg G

35-5000 was used in Immunohistochemistry to identify the roles of syndecan 3 and connexin 43 in the co-ordination of myometrial contractility.

Thu May 04 00:00:00 EDT 2006

Prolonged labour associated with lower expression of syndecan 3 and connexin 43 in human uterine tissue.

Reproductive biology and endocrinology : RB&E

Cluff AH,Byström B,Klimaviciute A,Dahlqvist C,Cebers G,Malmström A,Ekman-Ordeberg G

35-5000 was used in Immunohistochemistry to identify the roles of syndecan 3 and connexin 43 in the co-ordination of myometrial contractility.

Thu May 04 00:00:00 EDT 2006

Human and mouse microglia express connexin36, and functional gap junctions are formed between rodent microglia and neurons.

Journal of neuroscience research

Dobrenis K,Chang HY,Pina-Benabou MH,Woodroffe A,Lee SC,Rozental R,Spray DC,Scemes E

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Western Blot

Tue Nov 01 00:00:00 EST 2005

Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning.

Neuroscience

Kamasawa N,Sik A,Morita M,Yasumura T,Davidson KG,Nagy JI,Rash JE

Tue Feb 14 00:00:00 EST 2006

Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning.

Neuroscience

Kamasawa N,Sik A,Morita M,Yasumura T,Davidson KG,Nagy JI,Rash JE

Tue Feb 14 00:00:00 EST 2006

Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain.

The European journal of neuroscience

Penes MC,Li X,Nagy JI

Fri Jul 01 00:00:00 EDT 2005

The European journal of neuroscience

Penes MC,Li X,Nagy JI

Fri Jul 01 00:00:00 EDT 2005

The European journal of neuroscience

Penes MC,Li X,Nagy JI

Fri Jul 01 00:00:00 EDT 2005

Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb.

Journal of neurocytology

Rash JE,Davidson KG,Kamasawa N,Yasumura T,Kamasawa M,Zhang C,Michaels R,Restrepo D,Ottersen OP,Olson CO,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Thu Sep 01 00:00:00 EDT 2005

Identification of kit positive cells in the human urinary tract.

The Journal of urology

van der AA F,Roskams T,Blyweert W,Ost D,Bogaert G,De Ridder D

35-5000 was used in immunohistochemistry - frozen section to investigate the presence of interstitial cells in the human urinary tract

Tue Jun 01 00:00:00 EDT 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Tue Sep 14 00:00:00 EDT 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Tue Sep 14 00:00:00 EDT 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Tue Sep 14 00:00:00 EDT 2004

Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice.

Glia

Nagy JI,Ionescu AV,Lynn BD,Rash JE

Mon Dec 01 00:00:00 EST 2003

Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice.

Glia

Nagy JI,Ionescu AV,Lynn BD,Rash JE

Mon Dec 01 00:00:00 EST 2003

Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system.

The Journal of comparative neurology

Nagy JI,Ionescu AV,Lynn BD,Rash JE

Mon Sep 22 00:00:00 EDT 2003

Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system.

The Journal of comparative neurology

Nagy JI,Ionescu AV,Lynn BD,Rash JE

Mon Sep 22 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; ODD; ODOD; SDTY3; Syndactyly type III

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Gene Aliases: AU042049; AW546267; Cnx43; connexin43; Cx43; Cx43alpha1; Cxn-43; Gja-1; Gja1; Npm1

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UniProt ID: (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Mouse) 14609, (Rat) 24392

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Function(s)

signal transducer activity receptor binding gap junction channel activity protein binding ion transmembrane transporter activity SH3 domain binding protein domain specific binding transmembrane transporter activity PDZ domain binding beta-tubulin binding connexin binding scaffold protein binding gap junction channel activity involved in cell communication by electrical coupling gap junction

Process(es)

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Connexin 43 Monoclonal Antibody (3D8A5)

Connexin 43 Antibody (35-5000) in IHC

Product Details

35-5000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

In Situ Hybridization (ISH) (ISH)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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