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FIGURE: 1 / 1
Cox-2 Antibody (100-401-226) in WB
Rocklands anti-Cox2 is shown to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1ß induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
Product Details
100-401-226
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a fusion protein corresponding to the carboxy-terminus of human Cox-2.
Conjugate
Form
Liquid
Concentration
55 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antiserum is directed against human Cox-2. Two different isoforms of the enzyme are known, Cox-1 and Cox-2. A BLAST analysis was used to suggest cross-reactivity with Cox-2 in human, mouse and rat based on a homology with the immunizing sequence and should not cross react with Cox-1. Reactivity against homologues from other sources is not known. Reactivity of this antibody with Cox-2 from other species is unknown.
Target Information
COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2b; Cyclooxygenase-2; Glucocorticoid-regulated inflammatory cyclooxygenase; Gripghs; Macrophage activation-associated marker protein P71/73; PES-2; PGH synthase 2; PGHS-2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); TIS10 protein
Gene Aliases: COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; Pghs-b; Pghs2; PHS-2; PTGS2; TIS10
UniProt ID: (Human) P35354, (Rat) P35355, (Mouse) Q05769
Entrez Gene ID: (Human) 5743, (Rat) 29527, (Mouse) 19225
peroxidase activity
prostaglandin-endoperoxide synthase activity
protein binding
lipid binding
enzyme binding
heme binding
protein homodimerization activity
metal ion binding
arachidonate 15-lipoxygenase activity
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
oxidoreductase activity
dioxygenase activity
oxygenase
prostaglandin biosynthetic process
angiogenesis
prostaglandin metabolic process
nicotinamide metabolic process
movement of cell or subcellular component
inflammatory response
response to oxidative stress
embryo implantation
learning
memory
regulation of blood pressure
negative regulation of cell proliferation
response to fructose
response to manganese ion
response to lithium ion
positive regulation of vascular endothelial growth factor production
sensory perception of pain
cyclooxygenase pathway
lipoxygenase pathway
bone mineralization
ovulation
positive regulation of prostaglandin biosynthetic process
positive regulation of fever generation
positive regulation of synaptic plasticity
negative regulation of synaptic transmission, dopaminergic
response to estradiol
response to lipopolysaccharide
response to vitamin D
response to tumor necrosis factor
cellular response to UV
maintenance of blood-brain barrier
positive regulation of NF-kappaB import into nucleus
response to drug
hair cycle
positive regulation of apoptotic process
positive regulation of nitric oxide biosynthetic process
negative regulation of cell cycle
positive regulation of vasoconstriction
negative regulation of smooth muscle contraction
positive regulation of smooth muscle contraction
decidualization
positive regulation of smooth muscle cell proliferation
regulation of inflammatory response
brown fat cell differentiation
response to glucocorticoid
negative regulation of calcium ion transport
positive regulation of synaptic transmission, glutamatergic
oxidation-reduction process
response to fatty acid
cellular response to mechanical stimulus
cellular response to ATP
cellular response to hypoxia
cellular response to fluid shear stress
positive regulation of transforming growth factor beta production
positive regulation of cell migration involved in sprouting angiogenesis
positive regulation of fibroblast growth factor production
positive regulation of brown fat cell differentiation
positive regulation of platelet-derived growth factor production
cellular oxidant detoxification
positive regulation of cell proliferation
response to radiation
response to organic substance
response to organonitrogen compound
positive regulation of cell death
response to organic cyclic compound
response to cytokine
regulation of cell proliferation
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
keratinocyte differentiation
anagen
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