Product References
Cullin neddylation inhibitor attenuates hyperglycemia by enhancing hepatic insulin signaling through insulin receptor substrate stabilization.
Proceedings of the National Academy of Sciences of the United States of America
Chen C,Gu L,Matye DJ,Clayton YD,Hasan MN,Wang Y,Friedman JE,Li T
32-2400 was used in Knock Down, Western Blot to suggest that pharmacological inhibition of cullin neddylation represents a therapeutic approach for improving hepatic insulin signaling and lowering blood glucose.
Tue Feb 08 00:00:00 EST 2022
Cullin neddylation inhibitor attenuates hyperglycemia by enhancing hepatic insulin signaling through insulin receptor substrate stabilization.
Proceedings of the National Academy of Sciences of the United States of America
Chen C,Gu L,Matye DJ,Clayton YD,Hasan MN,Wang Y,Friedman JE,Li T
32-2400 was used in Knock Down, Western Blot to suggest that pharmacological inhibition of cullin neddylation represents a therapeutic approach for improving hepatic insulin signaling and lowering blood glucose.
Tue Feb 08 00:00:00 EST 2022
Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote mitotic protein translation and faithful cell division.
Nature communications
Clemm von Hohenberg K,Müller S,Schleich S,Meister M,Bohlen J,Hofmann TG,Teleman AA
32-2400 was used in Western Blot to provide a mechanism how the cell cycle regulates translation of a subset of mitotically relevant mRNAs during mitosis.
Thu Feb 03 00:00:00 EST 2022
Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote mitotic protein translation and faithful cell division.
Nature communications
Clemm von Hohenberg K,Müller S,Schleich S,Meister M,Bohlen J,Hofmann TG,Teleman AA
32-2400 was used in Western Blot to provide a mechanism how the cell cycle regulates translation of a subset of mitotically relevant mRNAs during mitosis.
Thu Feb 03 00:00:00 EST 2022
The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.
Molecular cell
Heider M,Eichner R,Stroh J,Morath V,Kuisl A,Zecha J,Lawatscheck J,Baek K,Garz AK,Rudelius M,Deuschle FC,Keller U,Lemeer S,Verbeek M,Götze KS,Skerra A,Weber WA,Buchner J,Schulman BA,Kuster B,Fernández-Sáiz V,Bassermann F
32-2400 was used in ELISA, Immunoprecipitation, Western Blot to establish the CRBN-AHA1-HSP90 axis in the biogenesis of transmembrane proteins, link IMiD activity to tumor metabolism, and nominate CD98hc and LAT1 as attractive diagnostic and therapeutic targets in MM.
Thu Mar 18 00:00:00 EDT 2021
The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.
Molecular cell
Heider M,Eichner R,Stroh J,Morath V,Kuisl A,Zecha J,Lawatscheck J,Baek K,Garz AK,Rudelius M,Deuschle FC,Keller U,Lemeer S,Verbeek M,Götze KS,Skerra A,Weber WA,Buchner J,Schulman BA,Kuster B,Fernández-Sáiz V,Bassermann F
32-2400 was used in ELISA, Immunoprecipitation, Western Blot to establish the CRBN-AHA1-HSP90 axis in the biogenesis of transmembrane proteins, link IMiD activity to tumor metabolism, and nominate CD98hc and LAT1 as attractive diagnostic and therapeutic targets in MM.
Thu Mar 18 00:00:00 EDT 2021
Shigella ubiquitin ligase IpaH7.8 targets gasdermin D for degradation to prevent pyroptosis and enable infection.
Cell host & microbe
Luchetti G,Roncaioli JL,Chavez RA,Schubert AF,Kofoed EM,Reja R,Cheung TK,Liang Y,Webster JD,Lehoux I,Skippington E,Reeder J,Haley B,Tan MW,Rose CM,Newton K,Kayagaki N,Vance RE,Dixit VM
32-2400 was used in Western Blotting to highlight a species-specific pathogen arms race focused on maintenance of host cell viability.
Wed Oct 13 00:00:00 EDT 2021
Loss of FBXO31-mediated degradation of DUSP6 dysregulates ERK and PI3K-AKT signaling and promotes prostate tumorigenesis.
Cell reports
Duan S,Moro L,Qu R,Simoneschi D,Cho H,Jiang S,Zhao H,Chang Q,de Stanchina E,Arbini AA,Pagano M
32-2400 was used in Western Blotting to highlight the relevance of the FBXO31-DUSP6 axis in the regulation of ERK- and PI3K-AKT-mediated signaling pathways, as well as its therapeutic potential in prostate cancer.
Tue Oct 19 00:00:00 EDT 2021
Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.
Nature communications
Dietachmayr M,Rathakrishnan A,Karpiuk O,von Zweydorf F,Engleitner T,Fernández-Sáiz V,Schenk P,Ueffing M,Rad R,Eilers M,Gloeckner CJ,Clemm von Hohenberg K,Bassermann F
32-2400 was used in Western Blot to find that CDC14B antagonizes CDK1-mediated activating mitotic phosphorylation of the deubiquitinase USP9X at serine residue 2563, which we show to be essential for USP9X to mediate mitotic survival.
Mon Mar 09 00:00:00 EDT 2020
Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.
Nature communications
Dietachmayr M,Rathakrishnan A,Karpiuk O,von Zweydorf F,Engleitner T,Fernández-Sáiz V,Schenk P,Ueffing M,Rad R,Eilers M,Gloeckner CJ,Clemm von Hohenberg K,Bassermann F
32-2400 was used in Western Blot to find that CDC14B antagonizes CDK1-mediated activating mitotic phosphorylation of the deubiquitinase USP9X at serine residue 2563, which we show to be essential for USP9X to mediate mitotic survival.
Mon Mar 09 00:00:00 EDT 2020
The Balance between Mono- and NEDD8-Chains Controlled by NEDP1 upon DNA Damage Is a Regulatory Module of the HSP70 ATPase Activity.
Cell reports
Bailly AP,Perrin A,Serrano-Macia M,Maghames C,Leidecker O,Trauchessec H,Martinez-Chantar ML,Gartner A,Xirodimas DP
Published figure using Cullin 1 monoclonal antibody (Product # 32-2400) in Western Blot
Tue Oct 01 00:00:00 EDT 2019
Pathogenic mutations in the ALS gene CCNF cause cytoplasmic mislocalization of Cyclin F and elevated VCP ATPase activity.
Human molecular genetics
Yu Y,Nakagawa T,Morohoshi A,Nakagawa M,Ishida N,Suzuki N,Aoki M,Nakayama K
32-2400 was used in Western Blotting to indicate that Cyclin F and VCP contribute to the same molecular pathway that facilitates TDP-43 aggregation.
Tue Oct 15 00:00:00 EDT 2019
Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression.
Molecular cell
Liu X,Reitsma JM,Mamrosh JL,Zhang Y,Straube R,Deshaies RJ
32-2400 was used in Western Blotting to develop a mathematical model for how dynamical assembly of Skp1⋅Cul1⋅F-box complexes is controlled and how this cycle is coupled to degradation of an SCF substrate.
Thu Mar 01 00:00:00 EST 2018
The ULK1-FBXW5-SEC23B nexus controls autophagy.
eLife
Jeong YT,Simoneschi D,Keegan S,Melville D,Adler NS,Saraf A,Florens L,Washburn MP,Cavasotto CN,Fenyö D,Cuervo AM,Rossi M,Pagano M
32-2400 was used in Western Blot to show that the F-box protein FBXW5 targets SEC23B, a component of COPII, for proteasomal degradation and that this event limits the autophagic flux in the presence of nutrients.
Mon Dec 31 00:00:00 EST 2018
NS5A Promotes Constitutive Degradation of IP3R3 to Counteract Apoptosis Induced by Hepatitis C Virus.
Cell reports
Kuchay S,Saeed M,Giorgi C,Li J,Hoffmann HH,Pinton P,Rice CM,Pagano M
32-2400 was used in Western Blotting to reveal an 1,4,5-trisphosphate-independent molecular mechanism through which hepatitis C virus promotes IP3R3 degradation, thereby inhibiting virus-induced apoptosis and establishing chronic infection.
Tue Oct 23 00:00:00 EDT 2018
Multiple UBXN family members inhibit retrovirus and lentivirus production and canonical NFκΒ signaling by stabilizing IκBα.
PLoS pathogens
Hu Y,O'Boyle K,Auer J,Raju S,You F,Wang P,Fikrig E,Sutton RE
32-2400 was used in Western Blotting to identify the mechanism used by multiple UBXN family members to inhibit retrovirus and lentivirus production, and canonical NFκΒ signaling.
Wed Feb 01 00:00:00 EST 2017
Geminin is an indispensable inhibitor of Cdt1 in mouse embryonic stem cells.
Genes to cells : devoted to molecular & cellular mechanisms
Hosogane M,Bosu L,Fukumoto E,Yamada H,Sato S,Nakayama K
32-2400 was used in Western Blotting to characterise the Geminin-Cdt1 axis in embryonic stem cells and compare it with that in somatic cells.
Sat Apr 01 00:00:00 EDT 2017
Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.
Nature medicine
Eichner R,Heider M,Fernández-Sáiz V,van Bebber F,Garz AK,Lemeer S,Rudelius M,Targosz BS,Jacobs L,Knorn AM,Slawska J,Platzbecker U,Germing U,Langer C,Knop S,Einsele H,Peschel C,Haass C,Keller U,Schmid B,Götze KS,Kuster B,Bassermann F
32-2400 was used in western blot to study disruption of the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity due to immunomodulatory drugs
Fri Jul 01 00:00:00 EDT 2016
Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps).
Nature protocols
Mark KG,Loveless TB,Toczyski DP
32-2400 was used in western blot to utilize tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps) to isolate ubiquitinated substrates
Mon Feb 01 00:00:00 EST 2016
USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.
EMBO molecular medicine
Engel K,Rudelius M,Slawska J,Jacobs L,Ahangarian Abhari B,Altmann B,Kurutz J,Rathakrishnan A,Fernández-Sáiz V,Brunner A,Targosz BS,Loewecke F,Gloeckner CJ,Ueffing M,Fulda S,Pfreundschuh M,Trümper L,Klapper W,Keller U,Jost PJ,Rosenwald A,Peschel C,Bassermann F
Published figure using Cullin 1 monoclonal antibody (Product # 32-2400) in Western Blot
Mon Aug 01 00:00:00 EDT 2016
USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.
EMBO molecular medicine
Engel K,Rudelius M,Slawska J,Jacobs L,Ahangarian Abhari B,Altmann B,Kurutz J,Rathakrishnan A,Fernández-Sáiz V,Brunner A,Targosz BS,Loewecke F,Gloeckner CJ,Ueffing M,Fulda S,Pfreundschuh M,Trümper L,Klapper W,Keller U,Jost PJ,Rosenwald A,Peschel C,Bassermann F
Published figure using Cullin 1 monoclonal antibody (Product # 32-2400) in Western Blot
Mon Aug 01 00:00:00 EDT 2016
Peptidic degron in EID1 is recognized by an SCF E3 ligase complex containing the orphan F-box protein FBXO21.
Proceedings of the National Academy of Sciences of the United States of America
Zhang C,Li X,Adelmant G,Dobbins J,Geisen C,Oser MG,Wucherpfenning KW,Marto JA,Kaelin WG
32-2400 was used in western blot to analyze recognition by an SCF E3 ligase complex containing the orphan F-box protein FBXO21 by peptidic degron in EID1
Tue Dec 15 00:00:00 EST 2015
Ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase (SCF) interact through multiple conformations.
The Journal of biological chemistry
Sandoval D,Hill S,Ziemba A,Lewis S,Kuhlman B,Kleiger G
32-2400 was used in western blot to study the interaction between ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase.
Fri Jan 09 00:00:00 EST 2015
Ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase (SCF) interact through multiple conformations.
The Journal of biological chemistry
Sandoval D,Hill S,Ziemba A,Lewis S,Kuhlman B,Kleiger G
32-2400 was used in western blot to study the interaction between ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase.
Fri Jan 09 00:00:00 EST 2015
F-box protein FBXL16 binds PP2A-B55α and regulates differentiation of embryonic stem cells along the FLK1+ lineage.
Molecular & cellular proteomics : MCP
Honarpour N,Rose CM,Brumbaugh J,Anderson J,Graham RL,Sweredoski MJ,Hess S,Coon JJ,Deshaies RJ
Published figure using Cullin 1 monoclonal antibody (Product # 32-2400) in Western Blot
Sat Mar 01 00:00:00 EST 2014
F-box protein FBXL16 binds PP2A-B55α and regulates differentiation of embryonic stem cells along the FLK1+ lineage.
Molecular & cellular proteomics : MCP
Honarpour N,Rose CM,Brumbaugh J,Anderson J,Graham RL,Sweredoski MJ,Hess S,Coon JJ,Deshaies RJ
Published figure using Cullin 1 monoclonal antibody (Product # 32-2400) in Western Blot
Sat Mar 01 00:00:00 EST 2014
Small molecule-induced mitochondrial disruption directs prostate cancer inhibition via UPR signaling.
Oncotarget
Rico-Bautista E,Zhu W,Kitada S,Ganapathy S,Lau E,Krajewski S,Ramirez J,Bush JA,Yuan Z,Wolf DA
32-2400 was used in western blot to analyze UPR signaling and its role in small molecule-induced mitochondrial disruption and prostate cancer inhibition
Thu Aug 01 00:00:00 EDT 2013
Early insights into the function of KIAA1199, a markedly overexpressed protein in human colorectal tumors.
PloS one
Tiwari A,Schneider M,Fiorino A,Haider R,Okoniewski MJ,Roschitzki B,Uzozie A,Menigatti M,Jiricny J,Marra G
32-2400 was used in immunoprecipitation to examine the localization and function of KIAA99 in human colorectal tumors.
Wed Mar 05 00:00:00 EST 2014
Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface.
Molecular cell
Duda DM,Olszewski JL,Tron AE,Hammel M,Lambert LJ,Waddell MB,Mittag T,DeCaprio JA,Schulman BA
Published figure using Cullin 1 monoclonal antibody (Product # 32-2400) in Western Blot
Fri Aug 10 00:00:00 EDT 2012
The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7.
Molecular cell
Tron AE,Arai T,Duda DM,Kuwabara H,Olszewski JL,Fujiwara Y,Bahamon BN,Signoretti S,Schulman BA,DeCaprio JA
Published figure using Cullin 1 monoclonal antibody (Product # 32-2400) in Western Blot
Fri Apr 13 00:00:00 EDT 2012
Cullin-3 regulates late endosome maturation.
Proceedings of the National Academy of Sciences of the United States of America
Huotari J,Meyer-Schaller N,Hubner M,Stauffer S,Katheder N,Horvath P,Mancini R,Helenius A,Peter M
Tue Jan 17 00:00:00 EST 2012
Designer reagents for mass spectrometry-based proteomics: clickable cross-linkers for elucidation of protein structures and interactions.
Analytical chemistry
Sohn CH,Agnew HD,Lee JE,Sweredoski MJ,Graham RL,Smith GT,Hess S,Czerwieniec G,Loo JA,Heath JR,Deshaies RJ,Beauchamp JL
Published figure using Cullin 1 monoclonal antibody (Product # 32-2400) in Western Blot
Tue Mar 20 00:00:00 EDT 2012
UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1α accumulation.
BMC biology
Bandau S,Knebel A,Gage ZO,Wood NT,Alexandru G
Thu Apr 26 00:00:00 EDT 2012
SCF(FBW7) regulates cellular apoptosis by targeting MCL1 for ubiquitylation and destruction.
Nature
Inuzuka H,Shaik S,Onoyama I,Gao D,Tseng A,Maser RS,Zhai B,Wan L,Gutierrez A,Lau AW,Xiao Y,Christie AL,Aster J,Settleman J,Gygi SP,Kung AL,Look T,Nakayama KI,DePinho RA,Wei W
32-2400 was used in western blot to study the mechanism by which the tumor-suppressor SCF(FBW7) regulates cellular apoptosis
Thu Mar 03 00:00:00 EST 2011
Control of chromosome stability by the beta-TrCP-REST-Mad2 axis.
Nature
Guardavaccaro D,Frescas D,Dorrello NV,Peschiaroli A,Multani AS,Cardozo T,Lasorella A,Iavarone A,Chang S,Hernando E,Pagano M
32-2400 was used in western blot to investigate the interaction between REST and beta-TrCP
Thu Mar 20 00:00:00 EDT 2008
