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Cyclin B1 Polyclonal Antibody

Cyclin B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

Cyclin B1 Antibody (28603-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using (Product # 28603-1-AP) (Cyclin B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cyclin B1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

28603-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000

View 31 publications 31 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

1:2,000

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Chicken, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CCNB1 Fusion Protein Ag29426

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

400 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Experimental cell research

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Experimental cell research

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Phosphorylation of VP1 Mediated by CDK1-Cyclin B1 Facilitates Infectious Bursal Disease Virus Replication.

Journal of virology

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Phosphorylation of VP1 Mediated by CDK1-Cyclin B1 Facilitates Infectious Bursal Disease Virus Replication.

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Bioinformatics

Protein Aliases: G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023

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Gene Aliases: CCNB; CCNB1

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UniProt ID: (Human) P14635

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Entrez Gene ID: (Human) 891

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Function(s)

cyclin-dependent protein serine/threonine kinase activity patched binding protein binding protein kinase binding histone kinase activity kinase activator

Process(es)

G2/M transition of mitotic cell cycle oocyte maturation in utero embryonic development negative regulation of protein phosphorylation protein complex assembly DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic spindle organization mitotic nuclear envelope disassembly mitotic metaphase plate congression spermatogenesis response to mechanical stimulus negative regulation of gene expression anaphase-promoting complex-dependent catabolic process positive regulation of mRNA 3'-end processing positive regulation of histone phosphorylation tissue regeneration response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of mitotic cell cycle response to DDT positive regulation of fibroblast proliferation digestive tract development cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of cell cycle positive regulation of attachment of spindle microtubules to kinetochore ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation regulation of chromosome condensation cellular response to iron(III) ion cellular response to fatty acid cellular response to organic cyclic compound cellular response to hypoxia regulation of mitotic cell cycle spindle assembly checkpoint histone H3-S10 phosphorylation involved in chromosome condensation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cyclin B1 Polyclonal Antibody

Cyclin B1 Antibody (28603-1-AP) in IHC (P)

Product Details

28603-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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