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Invitrogen
Cyclin E Recombinant Rabbit Monoclonal Antibody (8W7P8)
FIGURE: 1 / 1
Cyclin E Antibody (MA5-55261) in WB
Western Blot analysis of various lysates using Cyclin E Recombinant Monoclonal Antibody (Product # MA5-55261) at 1:10,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-55261
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8W7P8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Cyclin E1.
Conjugate
Form
Liquid
Concentration
0.75 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: AASALYHFSS SELMQKVSGY QWCDIENCVK WMVPFAMVIR ETGSSKLKHF RGVADEDAHN IQTHRDSLDL LDKARAKKAM LSEQNRASPL PSGLLTPPQS G
Target Information
Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cyclin E; cyclin Es; cyclin Et; E-S; G1/S-specific cyclin-E1
Gene Aliases: AW538188; CCNE; CCNE1; CycE1; CYCLE; pCCNE1
UniProt ID: (Human) P24864, (Mouse) Q61457
Entrez Gene ID: (Human) 898, (Rat) 25729, (Mouse) 12447
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
antral ovarian follicle growth
liver development
DNA replication initiation
protein phosphorylation
response to purine-containing compound
Wnt signaling pathway
androgen receptor signaling pathway
organ regeneration
cellular response to nutrient
response to estradiol
response to progesterone
response to vitamin E
response to cytokine
response to drug
response to ethanol
positive regulation of cell differentiation
regulation of protein kinase activity
positive regulation of transcription, DNA-templated
cell division
response to corticosterone
response to methylmercury
regulation of cell cycle
response to organonitrogen compound
response to organic cyclic compound
response to steroid hormone
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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