Invitrogen

Cyclophilin 40 Polyclonal Antibody

Cyclophilin 40 Antibody in Western Blot (WB)

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Cyclophilin 40 Antibody (PA1-028) in WB

Figure 2 shows a Western blot of CyPD on rat brain extract using Product # PA1-028.

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Product Details

PA1-028

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 13 publications 13 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues S(42) G N P L V Y L D V D A N(54) C of mature human CyPD.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2169135

Product Specific Information

PA1-028 detects cyclophilin D (CyPD) in rat, hamster, canine, mouse, and human tissues.

PA1-028 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects a ~15 kDa protein in rat liver and heart extract, and a ~37 kDa in rat brain extract.

The PA1-028 immunogen is a synthetic peptide corresponding to residues S(42) G N P L V Y L D V D A N(54) C of mature human CyPD. This peptide (Cat. # PEP-104) is available for use in neutralization and control experiments.

Target Information

This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ANT-dependent MPTP underlies necrotic myofiber death in muscular dystrophy.

Science advances

Bround MJ,Havens JR,York AJ,Sargent MA,Karch J,Molkentin JD

Published figure using Cyclophilin 40 polyclonal antibody (Product # PA1-028) in Western Blot

Fri Aug 25 00:00:00 EDT 2023

Heat shock protein 60 regulates yolk sac erythropoiesis in mice.

Cell death & disease

Duan Y,Wang H,Mitchell-Silbaugh K,Cai S,Fan F,Li Y,Tang H,Wang G,Fang X,Liu J,Jia N,Jing R,Ouyang K

PA1-028 was used in Immunohistochemistry (PFA) to demonstrate an essential role of HSP60 in regulating yolk sac cell survival partially via a mPTP-dependent mechanism.

Thu Oct 10 00:00:00 EDT 2019

Loss of hepatic LRPPRC alters mitochondrial bioenergetics, regulation of permeability transition and trans-membrane ROS diffusion.

Human molecular genetics

Cuillerier A,Honarmand S,Cadete VJJ,Ruiz M,Forest A,Deschênes S,Beauchamp C,Charron G,Rioux JD,Des Rosiers C,Shoubridge EA,Burelle Y

PA1-028 was used in Western Blotting to investigate the impact of the French-Canadian variant of Leigh Syndrome in the liver.

Tue Aug 15 00:00:00 EDT 2017

Mitochondrial permeability transition and its regulatory components are implicated in apoptosis of primary cultures of rat proximal tubular cells exposed to lead.

Archives of toxicology

Liu G,Wang ZK,Wang ZY,Yang DB,Liu ZP,Wang L

PA1-028 was used in western blot to expand our understanding of the mechanisms involved in Pb-induced mitochondrial apoptosis

Sun May 01 00:00:00 EDT 2016

Cardiac and mitochondrial dysfunction following acute pulmonary exposure to mountaintop removal mining particulate matter.

American journal of physiology. Heart and circulatory physiology

Nichols CE,Shepherd DL,Knuckles TL,Thapa D,Stricker JC,Stapleton PA,Minarchick VC,Erdely A,Zeidler-Erdely PC,Alway SE,Nurkiewicz TR,Hollander JM

Published figure using Cyclophilin 40 polyclonal antibody (Product # PA1-028) in Western Blot

Tue Dec 15 00:00:00 EST 2015

Cyclophilin D-sensitive mitochondrial permeability transition in adult human brain and liver mitochondria.

Journal of neurotrauma

Hansson MJ,Morota S,Chen L,Matsuyama N,Suzuki Y,Nakajima S,Tanoue T,Omi A,Shibasaki F,Shimazu M,Ikeda Y,Uchino H,Elmér E

PA1-028 was used in western blot to investigate the effect of CypD on mitochondrial permeability regulation in liver and brain

Sat Jan 01 00:00:00 EST 2011

Enhanced apoptotic propensity in diabetic cardiac mitochondria: influence of subcellular spatial location.

American journal of physiology. Heart and circulatory physiology

Williamson CL,Dabkowski ER,Baseler WA,Croston TL,Alway SE,Hollander JM

PA1-028 was used in western blot to study the mitochondrial apoptotic status of myocardium in diabetes mellitus

Mon Feb 01 00:00:00 EST 2010

Mitochondrial dysfunction in the type 2 diabetic heart is associated with alterations in spatially distinct mitochondrial proteomes.

American journal of physiology. Heart and circulatory physiology

Dabkowski ER,Baseler WA,Williamson CL,Powell M,Razunguzwa TT,Frisbee JC,Hollander JM

PA1-028 was used in western blot to investigate the association between spatially distinct mitochondrial proteome and its dysfunction in the type 2 diabetic heart

Sun Aug 01 00:00:00 EDT 2010

Developmental shift of cyclophilin D contribution to hypoxic-ischemic brain injury.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Wang X,Carlsson Y,Basso E,Zhu C,Rousset CI,Rasola A,Johansson BR,Blomgren K,Mallard C,Bernardi P,Forte MA,Hagberg H

PA1-028 was used in western blot to study the different roles of cyclophilin D in hypoxic-ischemic brain injury between immature and adult brains

Wed Feb 25 00:00:00 EST 2009

Mitochondria-specific transgenic overexpression of phospholipid hydroperoxide glutathione peroxidase (GPx4) attenuates ischemia/reperfusion-associated cardiac dysfunction.

Free radical biology & medicine

Dabkowski ER,Williamson CL,Hollander JM

PA1-028 was used in western blot to investigate the effect of phospholipid hydroperoxide glutathione peroxidase on ischemia/reperfusion-associated cardiac dysfunction

Mon Sep 15 00:00:00 EDT 2008

Effects of permeability transition inhibition and decrease in cytochrome c content on doxorubicin toxicity in K562 cells.

Oncogene

De Oliveira F,Chauvin C,Ronot X,Mousseau M,Leverve X,Fontaine E

PA1-028 was used in western blot to investigate the effects of permeability transition inhibition and decrease in cytochrome c content on doxorubicin toxicity in K562 cells

Thu Apr 27 00:00:00 EDT 2006

Muscle denervation promotes opening of the permeability transition pore and increases the expression of cyclophilin D.

The Journal of physiology

Csukly K,Ascah A,Matas J,Gardiner PF,Fontaine E,Burelle Y

PA1-028 was used in western blot to examine the effects of muscle denervation on the sensitivity of the PTP to calcium-induced opening and the expression of cyclophilin D

Sat Jul 01 00:00:00 EDT 2006

Properties of the permeability transition pore in mitochondria devoid of Cyclophilin D.

The Journal of biological chemistry

Basso E,Fante L,Fowlkes J,Petronilli V,Forte MA,Bernardi P

PA1-028 was used in western blot to study the permeability transition pore (PTP) and its modulation in vitro and in vivo.

Fri May 13 00:00:00 EDT 2005

Bax does not directly participate in the Ca(2+)-induced permeability transition of isolated mitochondria.

The Journal of biological chemistry

De Marchi U,Campello S,Szabò I,Tombola F,Martinou JC,Zoratti M

PA1-028 was used in western blot to study the role of Bax in the calcium-induced mitochondrial permeability transition.

Fri Sep 03 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: 40 kDa peptidyl-prolyl cis-trans isomerase; 40 kDa peptidyl-prolyl cis-trans isomerase D; cyclophilin 40; Cyclophilin D; Cyclophilin-40; Cyclophilin-related protein; Cyp 40; Cyp D; CYP-40; cytoplasmic cyclophilin D; EC 5.2.1.8; MGC33096; Peptidyl-prolyl cis-trans isomerase D; peptidylprolyl isomerase D; peptidylprolyl isomerase D (cyclophilin D); PPIase D; rotamase; Rotamase D; testicular tissue protein Li 147

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Gene Aliases: 4930564J03Rik; CYP-40; CYP40; CYPD; I79_011990; PPID; Ppidl; Ppif

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UniProt ID: (Human) Q08752, (Mouse) Q9CR16, (Rat) Q6DGG0

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Entrez Gene ID: (Dog) 475481, (Human) 5481, (Mouse) 67738, (Chinese hamster) 100752921, (Rat) 361967

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Function(s)

peptidyl-prolyl cis-trans isomerase activity protein binding transcription factor binding cyclosporin A binding estrogen receptor binding Hsp70 protein binding heat shock protein binding Hsp90 protein binding FK506 binding isomerase activity enzyme binding peptide binding chaperone

Process(es)

negative regulation of transcription from RNA polymerase II promoter protein peptidyl-prolyl isomerization protein folding protein complex assembly apoptotic process protein transport viral release from host cell lipid particle organization positive regulation of apoptotic process positive regulation of viral genome replication positive regulation of protein secretion chaperone-mediated protein folding cellular response to UV-A transport regulation of apoptotic process response to oxidative stress negative regulation of apoptotic process regulation of mitochondrial membrane potential

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cyclophilin 40 Polyclonal Antibody

Cyclophilin 40 Antibody (PA1-028) in WB

Product Details

PA1-028

Western Blot (WB)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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