Proteintech

Cyclophilin A Polyclonal Antibody

Cyclophilin A Antibody in Immunocytochemistry (ICC/IF)

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Cyclophilin A Antibody (10720-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using PPIA antibody 10720-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Cyclophilin A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cyclophilin A Antibody in Western Blot (WB)

Cyclophilin A Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

10720-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 6 publications 6 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cyclophilin A Fusion Protein Ag1120 (1-165 aa encoded by BC005320)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MVNPTVFFD IAVDGEPLGR VSFELFADKV PKTAENFRAL STGEKGFGYK GSCFHRIIPG FMCQGGDFTR HNGTGGKSIY GEKFEDENFI LKHTGPGILS MANAGPNTNG SQFFICTAKT EWLDGKHVVF GKVKEGMNIV EAMERFGSRN GKTSKKITIA DCGQLE (1-165 aa encoded by B C005320)

Target Information

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Construction and validation of a prognostic signature based on necroptosis-related genes in hepatocellular carcinoma.

PloS one

Peng YL,Wang LX,Li MY,Liu LP,Li RS

Wed Feb 22 00:00:00 EST 2023

Cyclophilin A binds to AKT1 and facilitates the tumorigenicity of Epstein-Barr virus by mediating the activation of AKT/mTOR/NF-κB positive feedback loop.

Virologica Sinica

Xin S,Liu L,Li Y,Yang J,Zuo L,Cao P,Yan Q,Li S,Yang L,Cui T,Lu J

Thu Dec 01 00:00:00 EST 2022

Cyclophilin A binds to AKT1 and facilitates the tumorigenicity of Epstein-Barr virus by mediating the activation of AKT/mTOR/NF-κB positive feedback loop.

Virologica Sinica

Xin S,Liu L,Li Y,Yang J,Zuo L,Cao P,Yan Q,Li S,Yang L,Cui T,Lu J

Thu Dec 01 00:00:00 EST 2022

Cyclophilin A binds to AKT1 and facilitates the tumorigenicity of Epstein-Barr virus by mediating the activation of AKT/mTOR/NF-κB positive feedback loop.

Virologica Sinica

Xin S,Liu L,Li Y,Yang J,Zuo L,Cao P,Yan Q,Li S,Yang L,Cui T,Lu J

Thu Dec 01 00:00:00 EST 2022

Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.

Gene

Vu TD,Ito N,Oshima K,Maruko A,Nishi A,Mizoguchi K,Odaguchi H,Kobayashi Y,Okada N

Fri Jul 01 00:00:00 EDT 2022

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Cell research

Zhang S,Huang W,Ren L,Ju X,Gong M,Rao J,Sun L,Li P,Ding Q,Wang J,Zhang QC

Sat Jan 01 00:00:00 EST 2022

Identification and validation of necroptosis-related prognostic gene signature and tumor immune microenvironment infiltration characterization in esophageal carcinoma.

BMC gastroenterology

Sun K,Hong JJ,Chen DM,Luo ZX,Li JZ

Fri Jul 15 00:00:00 EDT 2022

Cyclophilin A causes severe fever with thrombocytopenia syndrome virus-induced cytokine storm by regulating mitogen-activated protein kinase pathway.

Frontiers in microbiology

Huang H,Jin K,Ouyang K,Jiang Z,Yang Z,Hu N,Dai Y,Zhang Y,Zhang Q,Han Y,Zhao J,Lin H,Wang C,Wang C,Sun X,Lu D,Zhu J,Li J

Tue Jan 03 00:00:00 EST 2023

Defective cyclophilin A induces TDP-43 proteinopathy: implications for amyotrophic lateral sclerosis and frontotemporal dementia.

Brain : a journal of neurology

Pasetto L,Grassano M,Pozzi S,Luotti S,Sammali E,Migazzi A,Basso M,Spagnolli G,Biasini E,Micotti E,Cerovic M,Carli M,Forloni G,De Marco G,Manera U,Moglia C,Mora G,Traynor BJ,Chiò A,Calvo A,Bonetto V

Fri Dec 31 00:00:00 EST 2021

Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis.

Molecular neurodegeneration

Pasetto L,Callegaro S,Corbelli A,Fiordaliso F,Ferrara D,Brunelli L,Sestito G,Pastorelli R,Bianchi E,Cretich M,Chiari M,Potrich C,Moglia C,Corbo M,Sorarù G,Lunetta C,Calvo A,Chiò A,Mora G,Pennuto M,Quattrone A,Rinaldi F,D'Agostino VG,Basso M,Bonetto V

Tue Aug 10 00:00:00 EDT 2021

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Science advances

Asamitsu S,Yabuki Y,Ikenoshita S,Kawakubo K,Kawasaki M,Usuki S,Nakayama Y,Adachi K,Kugoh H,Ishii K,Matsuura T,Nanba E,Sugiyama H,Fukunaga K,Shioda N

Fri Jan 01 00:00:00 EST 2021

Tacrolimus ameliorates podocyte injury by restoring FK506 binding protein 12 (FKBP12) at actin cytoskeleton.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yasuda H,Fukusumi Y,Ivanov V,Zhang Y,Kawachi H

Mon Nov 01 00:00:00 EDT 2021

Cyclophilin A inhibits trophoblast migration and invasion in vitro and vivo through p38/ERK/JNK pathways and causes features of preeclampsia in mice.

Life sciences

Hu H,Jiang J,Chen Q,Wei S,Liu M,Chen X,Fan C,Ma J,Chen W,Wang X,Zhong M

Sun Nov 15 00:00:00 EST 2020

Overexpression of Cyclophilin A in Human Periapical Lesions.

Journal of endodontics

Wang Y,Liu G,Zhang J,Zhu L,Yu J,Zhu S,Lv F,Peng B

Sun Dec 01 00:00:00 EST 2019

Epstein-Barr Virus Nuclear Antigen 1 Recruits Cyclophilin A to Facilitate the Replication of Viral DNA Genome.

Frontiers in microbiology

Xin S,Du S,Liu L,Xie Y,Zuo L,Yang J,Hu J,Yue W,Zhang J,Cao P,Zhu F,Lu J

Thu Oct 01 00:00:00 EDT 2020

Exosomal cyclophilin A as a novel noninvasive biomarker for Epstein-Barr virus associated nasopharyngeal carcinoma.

Cancer medicine

Liu L,Zuo L,Yang J,Xin S,Zhang J,Zhou J,Li G,Tang J,Lu J

Sat Jun 01 00:00:00 EDT 2019

Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.

Oncotarget

Tao Y,Wang K,Chen Z,Long L,Wu Q,Cui F,Zhu L,Xiang M,Jiang Y,Liang Y,Qiu S,Xiao Z,Yi B

Tue Apr 25 00:00:00 EDT 2017

Downregulation of Peptidylprolyl isomerase A promotes cell death and enhances doxorubicin-induced apoptosis in hepatocellular carcinoma.

Gene

Cheng S,Luo M,Ding C,Peng C,Lv Z,Tong R,Xiao H,Xie H,Zhou L,Wu J,Zheng S

Mon Oct 10 00:00:00 EDT 2016

Downregulation of Peptidylprolyl isomerase A promotes cell death and enhances doxorubicin-induced apoptosis in hepatocellular carcinoma.

Gene

Cheng S,Luo M,Ding C,Peng C,Lv Z,Tong R,Xiao H,Xie H,Zhou L,Wu J,Zheng S

Mon Oct 10 00:00:00 EDT 2016

Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells.

Biochemical and biophysical research communications

Wu P,Zhu X,Jin W,Hao S,Liu Q,Zhang L

Fri May 01 00:00:00 EDT 2015

Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells.

Biochemical and biophysical research communications

Wu P,Zhu X,Jin W,Hao S,Liu Q,Zhang L

Fri May 01 00:00:00 EDT 2015

Proteomic methods reveal cyclophilin A function as a host restriction factor against rotavirus infection.

Proteomics

He H,Mou Z,Li W,Fei L,Tang Y,Zhang J,Yan P,Chen Z,Yang X,Shen Z,Li J,Wu Y

Mon Apr 01 00:00:00 EDT 2013

Proteomic methods reveal cyclophilin A function as a host restriction factor against rotavirus infection.

Proteomics

He H,Mou Z,Li W,Fei L,Tang Y,Zhang J,Yan P,Chen Z,Yang X,Shen Z,Li J,Wu Y

Mon Apr 01 00:00:00 EDT 2013

Clinical significance of Keap1 and Nrf2 in oral squamous cell carcinoma.

PloS one

Huang CF,Zhang L,Ma SR,Zhao ZL,Wang WM,He KF,Zhao YF,Zhang WF,Liu B,Sun ZJ

Tue Sep 16 00:00:00 EDT 2014

Clinical significance of Keap1 and Nrf2 in oral squamous cell carcinoma.

PloS one

Huang CF,Zhang L,Ma SR,Zhao ZL,Wang WM,He KF,Zhao YF,Zhang WF,Liu B,Sun ZJ

Tue Sep 16 00:00:00 EDT 2014

Association of increased ligand cyclophilin A and receptor CD147 with hypoxia, angiogenesis, metastasis and prognosis of tongue squamous cell carcinoma.

Histopathology

Huang C,Sun Z,Sun Y,Chen X,Zhu X,Fan C,Liu B,Zhao Y,Zhang W

Sun Apr 01 00:00:00 EDT 2012

Association of increased ligand cyclophilin A and receptor CD147 with hypoxia, angiogenesis, metastasis and prognosis of tongue squamous cell carcinoma.

Histopathology

Huang C,Sun Z,Sun Y,Chen X,Zhu X,Fan C,Liu B,Zhao Y,Zhang W

Sun Apr 01 00:00:00 EDT 2012

Cyclophilin A secreted from fibroblast-like synoviocytes is involved in the induction of CD147 expression in macrophages of mice with collagen-induced arthritis.

Journal of inflammation (London, England)

Nishioku T,Dohgu S,Koga M,Machida T,Watanabe T,Miura T,Tsumagari K,Terasawa M,Yamauchi A,Kataoka Y

Tue Nov 20 00:00:00 EST 2012

Cyclophilin A secreted from fibroblast-like synoviocytes is involved in the induction of CD147 expression in macrophages of mice with collagen-induced arthritis.

Journal of inflammation (London, England)

Nishioku T,Dohgu S,Koga M,Machida T,Watanabe T,Miura T,Tsumagari K,Terasawa M,Yamauchi A,Kataoka Y

Tue Nov 20 00:00:00 EST 2012

Cyclophilin A secreted from fibroblast-like synoviocytes is involved in the induction of CD147 expression in macrophages of mice with collagen-induced arthritis.

Journal of inflammation (London, England)

Nishioku T,Dohgu S,Koga M,Machida T,Watanabe T,Miura T,Tsumagari K,Terasawa M,Yamauchi A,Kataoka Y

Tue Nov 20 00:00:00 EST 2012

Cyclophilin A secreted from fibroblast-like synoviocytes is involved in the induction of CD147 expression in macrophages of mice with collagen-induced arthritis.

Journal of inflammation (London, England)

Nishioku T,Dohgu S,Koga M,Machida T,Watanabe T,Miura T,Tsumagari K,Terasawa M,Yamauchi A,Kataoka Y

Tue Nov 20 00:00:00 EST 2012

Increased expression of peroxiredoxin 6 and cyclophilin A in squamous cell carcinoma of the tongue.

Oral diseases

Huang CF,Sun ZJ,Zhao YF,Chen XM,Jia J,Zhang WF

Fri Apr 01 00:00:00 EDT 2011

Increased expression of peroxiredoxin 6 and cyclophilin A in squamous cell carcinoma of the tongue.

Oral diseases

Huang CF,Sun ZJ,Zhao YF,Chen XM,Jia J,Zhang WF

Fri Apr 01 00:00:00 EDT 2011

CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.

Journal of virology

Watanabe A,Yoneda M,Ikeda F,Terao-Muto Y,Sato H,Kai C

Sat May 01 00:00:00 EDT 2010

CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.

Journal of virology

Watanabe A,Yoneda M,Ikeda F,Terao-Muto Y,Sato H,Kai C

Sat May 01 00:00:00 EDT 2010

CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.

Journal of virology

Watanabe A,Yoneda M,Ikeda F,Terao-Muto Y,Sato H,Kai C

Sat May 01 00:00:00 EDT 2010

CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.

Journal of virology

Watanabe A,Yoneda M,Ikeda F,Terao-Muto Y,Sato H,Kai C

Sat May 01 00:00:00 EDT 2010

Hepatitis B virus (HBV) surface antigen interacts with and promotes cyclophilin a secretion: possible link to pathogenesis of HBV infection.

Journal of virology