Invitrogen

Cyclophilin F Monoclonal Antibody (E11AE12BD4)

Cyclophilin F Antibody in Immunocytochemistry (ICC/IF)

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Cyclophilin F Antibody (45-5900) in ICC/IF

Immunofluorescent analysis of Cyclophilin F in MRC5 cells using a Cyclophilin F Monoclonal antibody (Product # 45-5900) at a concentration of 1 µg/mL. Cultured Human embryonic lung-derived fibroblasts (strain MRC5), were fixed, treated for heat-induced antigen retrieval, and permeabilized. Detection was perfomed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/... View More

Cyclophilin F Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cyclophilin F Antibody in Western Blot (WB)

Cyclophilin F Antibody in Flow Cytometry (Flow)

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Product Details

45-5900

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

1 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

E11AE12BD4

Immunogen

Recombinant rat Cyclophilin F

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2533820

Product Specific Information

Positive controls: Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, HepG2 cells; Cultured Human embryonic lung-derived fibroblasts (strain MRC5); Human cerebellum tissue; HL60 cells.

Target Information

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inner mitochondrial membrane protein MPV17 mutant mice display increased myocardial injury after ischemia/reperfusion.

American journal of translational research

Madungwe NB,Feng Y,Imam Aliagan A,Tombo N,Kaya F,Bopassa JC

45-5900 was used in Immunoprecipitation, Western Blot to investigate the role and mechanism of action of MPV17 in cardiac ischemia/reperfusion injury.

Fri May 07 00:00:00 EDT 2021

Inner mitochondrial membrane protein MPV17 mutant mice display increased myocardial injury after ischemia/reperfusion.

American journal of translational research

Madungwe NB,Feng Y,Imam Aliagan A,Tombo N,Kaya F,Bopassa JC

45-5900 was used in Immunoprecipitation, Western Blot to investigate the role and mechanism of action of MPV17 in cardiac ischemia/reperfusion injury.

Fri May 07 00:00:00 EDT 2021

Deficiency of miR-208a Exacerbates CCl₄-Induced Acute Liver Injury in Mice by Activating Cell Death Pathways.

Hepatology communications

Bala S,Calenda CD,Catalano D,Babuta M,Kodys K,Nasser IA,Vidal B,Szabo G

45-5900 was used in Western Blotting to demonstrate a new role of microRNA (miR)-208a in acute liver injury.

Thu Oct 01 00:00:00 EDT 2020

Deficiency of miR-208a Exacerbates CCl₄-Induced Acute Liver Injury in Mice by Activating Cell Death Pathways.

Hepatology communications

Bala S,Calenda CD,Catalano D,Babuta M,Kodys K,Nasser IA,Vidal B,Szabo G

45-5900 was used in Western Blotting to demonstrate a new role of microRNA (miR)-208a in acute liver injury.

Thu Oct 01 00:00:00 EDT 2020

Liproxstatin-1 protects the mouse myocardium against ischemia/reperfusion injury by decreasing VDAC1 levels and restoring GPX4 levels.

Biochemical and biophysical research communications

Feng Y,Madungwe NB,Imam Aliagan AD,Tombo N,Bopassa JC

45-5900 was used in Western Blotting to investigate the role and mechanism of liproxstatin-1(Lip-1) in reducing cell death in the ischemic myocardium.

Tue Dec 10 00:00:00 EST 2019

Bioinformatics

Protein Aliases: cyclophilin 3; Cyclophilin D; Cyclophilin F; CyP-D; CyP-M; Mitochondrial cyclophilin; mitochondrial Cyclophilin D; mitochondrial peptidyl-prolyl cis-trans isomerase; Peptidyl-prolyl cis-trans isomerase F; Peptidyl-prolyl cis-trans isomerase F, mitochondrial; peptidyl-prolyl cis-trans isomerase, mitochondrial; peptidylprolyl isomerase F; PPIase F; Rotamase F

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Gene Aliases: AW457192; CyP-D; CyP-F; CyP-M; CYP3; CypD; PPIase; PPIF

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UniProt ID: (Human) P30405, (Bovine) P30404, (Rat) P29117, (Mouse) Q99KR7

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Entrez Gene ID: (Human) 10105, (Bovine) 414346, (Rat) 282819, (Mouse) 105675

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Function(s)

peptidyl-prolyl cis-trans isomerase activity protein binding cyclosporin A binding peptide binding isomerase activity chaperone

Process(es)

protein peptidyl-prolyl isomerization response to ischemia protein folding apoptotic mitochondrial changes regulation of proton-transporting ATPase activity, rotational mechanism regulation of necrotic cell death negative regulation of ATPase activity negative regulation of apoptotic process regulation of mitochondrial membrane permeability necroptotic process cellular response to hydrogen peroxide cellular response to arsenic-containing substance cellular response to calcium ion positive regulation of release of cytochrome c from mitochondria negative regulation of release of cytochrome c from mitochondria negative regulation of oxidative phosphorylation regulation of mitochondrial membrane permeability involved in programmed necrotic cell death negative regulation of oxidative phosphorylation uncoupler activity negative regulation of intrinsic apoptotic signaling pathway apoptotic process mitochondrial outer membrane permeabilization involved in programmed cell death response to oxidative stress regulation of apoptotic process programmed cell death

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cyclophilin F Monoclonal Antibody (E11AE12BD4)

Cyclophilin F Antibody (45-5900) in ICC/IF

Product Details

45-5900

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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