Invitrogen

Cytochrome C Monoclonal Antibody (37BA11)

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

7 Published Figures

16 References

View all (68) Cytochrome C antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

Cytochrome C Antibody (45-6100) in ICC/IF

Immunofluorescence analysis of Cytochrome C was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytochrome C Monoclonal Antibody (37BA11) (Product # 45-6100) at 1 µg/mL in 0.1% BSA, incubated at 4 deg... View More

Cytochrome C Antibody in Western Blot (WB)

Cytochrome C Antibody in Flow Cytometry (Flow)

Cytochrome C Antibody in Immunohistochemistry (IHC)

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Product Details

45-6100

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-1 µg/mL

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/mL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, C. elegans, Human, Mouse, Rat

Published species

Human, Mouse, Rat, Yeast

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

37BA11

Immunogen

Bovine heart Cytochrome C

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2533821

Product Specific Information

45-6100 was used successfully in the western blot analysis of cytochrome C using human breast cancer cell line lysates.

Positive control: Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.

WB: Predicted molecular weight: 14 kDa. ICC/IF: Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

Evidence-based complementary and alternative medicine : eCAM

Cao YJ,Li HZ,Zhao J,Sun YM,Jin XW,Lv SQ,Luo JY,Fang XX,Wen WB,Liao JB

45-6100 was used in Western Blotting to investigate the mechanisms by which Jian-Gan-Xiao-Zhi decoction improve nonalcoholic fatty liver disease.

Tue Jul 19 00:00:00 EDT 2022

Impaired disassembly of the axon initial segment restricts mitochondrial entry into damaged axons.

The EMBO journal

Kiryu-Seo S,Matsushita R,Tashiro Y,Yoshimura T,Iguchi Y,Katsuno M,Takahashi R,Kiyama H

Published figure using Cytochrome C monoclonal antibody (Product # 45-6100) in Immunohistochemistry

Mon Oct 17 00:00:00 EDT 2022

Axonal injury alters the extracellular glial environment of the axon initial segment and allows substantial mitochondrial influx into axon initial segment.

The Journal of comparative neurology

Tamada H,Kiryu-Seo S,Sawada S,Kiyama H

45-6100 was used in Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Western Blot to conclude that axonal injury changes the extracellular glial environment surrounding the axon initial segment (AIS) and intracellular mitochondrial localization in the AIS.

Mon Nov 01 00:00:00 EDT 2021

16-Hydroxycleroda-3,13-dien-15,16-olide Induces Apoptosis in Human Bladder Cancer Cells through Cell Cycle Arrest, Mitochondria ROS Overproduction, and Inactivation of EGFR-Related Signalling Pathways.

Molecules (Basel, Switzerland)

Chen YC,Wang PY,Huang BM,Chen YJ,Lee WC,Chen YC

45-6100 was used in Western Blotting to investigate the mechanisms of 16-hydroxycleroda-3,13-dien-15,16-olide-induced apoptosis in T24 human bladder cancer cells.

Sun Aug 30 00:00:00 EDT 2020

Molecules (Basel, Switzerland)

Chen YC,Wang PY,Huang BM,Chen YJ,Lee WC,Chen YC

45-6100 was used in Western Blotting to investigate the mechanisms of 16-hydroxycleroda-3,13-dien-15,16-olide-induced apoptosis in T24 human bladder cancer cells.

Sun Aug 30 00:00:00 EDT 2020

MicroRNA-325-3p Facilitates Immune Escape of Mycobacterium tuberculosis through Targeting LNX1 via NEK6 Accumulation to Promote Anti-Apoptotic STAT3 Signaling.

mBio

Fu B,Xue W,Zhang H,Zhang R,Feldman K,Zhao Q,Zhang S,Shi L,Pavani KC,Nian W,Lin X,Wu H

45-6100 was used in Western Blotting to contribution to the understanding of the immune escape of M. tuberculosis, and they provide a theoretical basis for the development of therapeutic approaches for drug-resistant TB.

Tue Jun 02 00:00:00 EDT 2020

DNA methylation and histone deacetylation regulating insulin sensitivity due to chronic cold exposure.

Cryobiology

Wang X,Wang L,Sun Y,Li R,Deng J,Deng J

456100 was used in immunohistochemistry and western blot to assess the causal relationship between chronic cold exposure and insulin resistance and the mechanisms of how DNA methylation and histone deacetylation regulate cold-reduced insulin resistance

Wed Feb 01 00:00:00 EST 2017

A versatile tandem RNA isolation procedure to capture in vivo formed mRNA-protein complexes.

Methods (San Diego, Calif.)

Matia-González AM,Iadevaia V,Gerber AP

45-6100 was used in Western Blotting to escribe a tandem RNA isolation procedure (TRIP) that enables purification of in vivo formed messenger ribonucleoprotein (mRNP) complexes.

Sat Apr 15 00:00:00 EDT 2017

Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM.

The Journal of comparative neurology

Tamada H,Kiryu-Seo S,Hosokawa H,Ohta K,Ishihara N,Nomura M,Mihara K,Nakamura KI,Kiyama H

45-6100 was used in Flow Cytometry, Immunocytochemistry, Western Blot, Immunohistochemistry to demonstrate both 3D images of whole organelle and the intra-organellar structure simultaneously.

Tue Aug 01 00:00:00 EDT 2017

A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain.

Neurobiology of aging

Lauritzen KH,Hasan-Olive MM,Regnell CE,Kleppa L,Scheibye-Knudsen M,Gjedde A,Klungland A,Bohr VA,Storm-Mathisen J,Bergersen LH

45-6100 was used in immunohistochemistry - paraffin section to test if the severe neurodegeneration observed in mutUNG1-expressing mice is prevented by a ketogenic diet

Thu Dec 01 00:00:00 EST 2016

Conserved mRNA-binding proteomes in eukaryotic organisms.

Nature structural & molecular biology

Matia-González AM,Laing EE,Gerber AP

Published figure using Cytochrome C monoclonal antibody (Product # 45-6100) in Western Blot

Tue Dec 01 00:00:00 EST 2015

Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.

Nature communications

Sharma P,Abbasi C,Lazic S,Teng ACT,Wang D,Dubois N,Ignatchenko V,Wong V,Liu J,Araki T,Tiburcy M,Ackerley C,Zimmermann WH,Hamilton R,Sun Y,Liu PP,Keller G,Stagljar I,Scott IC,Kislinger T,Gramolini AO

45-6100 was used in western blot to investigate the impact of Tmem65 on heart development

Fri Sep 25 00:00:00 EDT 2015

Specific acetylation of p53 by HDAC inhibition prevents DNA damage-induced apoptosis in neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Brochier C,Dennis G,Rivieccio MA,McLaughlin K,Coppola G,Ratan RR,Langley B

45-6100 was used in western blot to investigate the effect of p53 acetylation on DNA damage-induced neuronal apoptosis.

Wed May 15 00:00:00 EDT 2013

Photothermal confocal spectromicroscopy of multiple cellular chromophores and fluorophores.

Biophysical journal

Nedosekin DA,Galanzha EI,Ayyadevara S,Shmookler Reis RJ,Zharov VP

Published figure using Cytochrome C monoclonal antibody (Product # 45-6100) in Immunofluorescence

Wed Feb 08 00:00:00 EST 2012

Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1.

The EMBO journal

Althoff T,Mills DJ,Popot JL,Kühlbrandt W

45-6100 was used in western blot to report the 19-A 3D map of the 1.7-MDa amphipol-solubilized supercomplex I(1)III(2)IV(1) isolated from bovine heart

Fri Sep 09 00:00:00 EDT 2011

Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses.

DNA repair

Lauritzen KH,Cheng C,Wiksen H,Bergersen LH,Klungland A

45-6100 was used in immunohistochemistry - paraffin section to investigate the effects of mitochondrial DNA damage on hippocampal neurons.

Fri Jun 10 00:00:00 EDT 2011

Analysis of mouse models of cytochrome c oxidase deficiency owing to mutations in Sco2.

Human molecular genetics

Yang H,Brosel S,Acin-Perez R,Slavkovich V,Nishino I,Khan R,Goldberg IJ,Graziano J,Manfredi G,Schon EA

Published figure using Cytochrome C monoclonal antibody (Product # 45-6100) in Western Blot

Fri Jan 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1

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Gene Aliases: CYC; CYCS; CYCSA; cytc; HCS; THC4

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UniProt ID: (Bovine) P62894, (Human) P99999, (Mouse) P62897, (Rat) P62898

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Entrez Gene ID: (Bovine) 510767, (Human) 54205, (Mouse) 13063, (Rat) 25309

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Function(s)

electron carrier activity heme binding metal ion binding protein serine/threonine phosphatase activity protein binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity iron ion binding lipid binding

Process(es)

apoptotic process response to reactive oxygen species mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic DNA fragmentation hydrogen peroxide metabolic process ATP synthesis coupled electron transport response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytochrome C Monoclonal Antibody (37BA11)

Cytochrome C Antibody (45-6100) in ICC/IF

Product Details

45-6100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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