Invitrogen

Cytochrome C Monoclonal Antibody (6H2.B4)

Advanced Verification

IP-mass spectrometry

1 Published Figure

11 References

View all (68) Cytochrome C antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

Cytochrome C Antibody (33-8200) in ICC/IF

Immunofluorescent analysis of Cytochrome C (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a Cytochrome C mouse monoclonal antibody (Product # 33-8200) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H... View More

Cytochrome C Antibody in Western Blot (WB)

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Product Details

33-8200

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5-20 µg/mL

View 6 publications 6 publications

ELISA (ELISA)

0.1-1 µg/mL

Immunoprecipitation (IP)

15 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Rabbit, Rat

Published species

Chicken, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6H2.B4

Immunogen

Rat Cytochrome C.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533141

Product Specific Information

This antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulation of mitochondrial proteostasis by the proton gradient.

The EMBO journal

Patron M,Tarasenko D,Nolte H,Kroczek L,Ghosh M,Ohba Y,Lasarzewski Y,Ahmadi ZA,Cabrera-Orefice A,Eyiama A,Kellermann T,Rugarli EI,Brandt U,Meinecke M,Langer T

33-8200 was used in Immunocytochemistry-immunofluorescence to use a multiproteomic approach to demonstrate the regulation of the m-AAA protease AFG3L2 by the mitochondrial proton gradient, coupling mitochondrial protein turnover to the energetic status of mitochondria.

Tue Aug 16 00:00:00 EDT 2022

Enhancing Proteotoxic Stress in Leiomyosarcoma Cells Triggers Mitochondrial Dysfunctions, Cell Death, and Antitumor Activity in vivo.

Molecular cancer therapeutics

Iuliano L,Drioli S,Pignochino Y,Cafiero CM,Minisini M,D'Este F,Picco R,Dalla E,Giordano G,Grignani G,Di Giorgio E,Benedetti F,Felluga F,Brancolini C

33-8200 was used in Immunocytochemistry-immunoflourescence to evaluate the induction of proteotoxic stress as a possible strategy to kill leiomyosarcoma cells in a therapeutic perspective.

Tue Jun 01 00:00:00 EDT 2021

Degradation of altered mitochondria by autophagy is impaired in Lafora disease.

The FEBS journal

Lahuerta M,Aguado C,Sánchez-Martín P,Sanz P,Knecht E

33-8200 was used in Immunocytochemistry-immunoflourescence to show the autophagic degradation of altered mitochondria is impaired in LD, which is due to a partial defect in the autophagic response and not in the canonical mitophagy signalling pathways.

Fri Jun 01 00:00:00 EDT 2018

Intracellular glycolysis in brown adipose tissue is essential for optogenetically induced nonshivering thermogenesis in mice.

Scientific reports

Jeong JH,Chang JS,Jo YH

Published figure using Cytochrome C monoclonal antibody (Product # 33-8200) in Western Blot

Fri Apr 27 00:00:00 EDT 2018

Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability.

Nature communications

Takaki T,Montagner M,Serres MP,Le Berre M,Russell M,Collinson L,Szuhai K,Howell M,Boulton SJ,Sahai E,Petronczki M

33-8200 was used in Immunocytochemistry-immunoflourescence to identify two subunits of the myosin phosphatase complex as proteins that safeguard nuclear integrity.

Mon Jul 24 00:00:00 EDT 2017

Loss of outer retinal neurons and circuitry alterations in the DBA/2J mouse.

Investigative ophthalmology & visual science

Fernández-Sánchez L,de Sevilla Müller LP,Brecha NC,Cuenca N

33-8200 was used in Immunohistochemistry to evaluate possible morphological changes in the outer retina of the DBA/2J mouse concomitant with disease progression and aging, based on the reduction of both the a- and b-waves and photopic flicker ERGs in this mouse line.

Tue Aug 12 00:00:00 EDT 2014

Parkinson's disease transgenic mitochondrial cybrids generate Lewy inclusion bodies.

Journal of neurochemistry

Trimmer PA,Borland MK,Keeney PM,Bennett JP,Parker WD

33-8200 was used in immunocytochemistry to report the generation of fibrillar and vesicular inclusions in a long-term cybrid cell culture model used to study Parkinson's disease.

Sun Feb 01 00:00:00 EST 2004

Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.

Journal of cell science

Love DC,Kochan J,Cathey RL,Shin SH,Hanover JA

Sat Feb 15 00:00:00 EST 2003

Afferent regulation of cytochrome-c and active caspase-9 in the avian cochlear nucleus.

Neuroscience

Wilkinson BL,Elam JS,Fadool DA,Hyson RL

33-8200 was used in immunohistochemistry (free floating) to study apoptosis of nucleus magnocellularis neurons.

Mon Nov 10 00:00:00 EST 2003

The Toxoplasma gondii protein ROP2 mediates host organelle association with the parasitophorous vacuole membrane.

The Journal of cell biology

Sinai AP,Joiner KA

Mon Jul 09 00:00:00 EDT 2001

Elevated AKT activity protects the prostate cancer cell line LNCaP from TRAIL-induced apoptosis.

The Journal of biological chemistry

Nesterov A,Lu X,Johnson M,Miller GJ,Ivashchenko Y,Kraft AS

Fri Apr 06 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: CG17903-PA; CG17903-PB; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p

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Gene Aliases: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4

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UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029

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Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996, (Rabbit) 100341175, (Rabbit) 100357840

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Function(s)

protein serine/threonine phosphatase activity protein binding heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding electron carrier activity iron ion binding lipid binding

Process(es)

response to reactive oxygen species mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic DNA fragmentation apoptotic process hydrogen peroxide metabolic process ATP synthesis coupled electron transport oxidative phosphorylation positive regulation of cysteine-type endopeptidase activity involved in apoptotic process oxidation-reduction process response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration

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Cytochrome C Monoclonal Antibody (6H2.B4)

Cytochrome C Antibody (33-8200) in ICC/IF

Product Details

33-8200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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