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Invitrogen
Cytochrome C Monoclonal Antibody (6H2), FITC, eBioscience™
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Product Details
11-6601-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
6H2
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_465365
Product Specific Information
Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c.
Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis.
Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1
Gene Aliases: CYC; CYCS; CYCSA; HCS; THC4
UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898
Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309
response to reactive oxygen species
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
protein dephosphorylation
mitochondrion organization
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cellular respiration
intrinsic apoptotic signaling pathway
apoptotic DNA fragmentation
apoptotic process
hydrogen peroxide metabolic process
ATP synthesis coupled electron transport
response to ischemia
response to oxidative stress
negative regulation of hydrogen peroxide biosynthetic process
glial cell apoptotic process
positive regulation of cellular respiration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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