Invitrogen

Cytochrome C Monoclonal Antibody (7H8.2C12)

Cytochrome C Antibody in Western Blot (WB)

FIGURE: 1 / 13

Cytochrome C Antibody (33-8500) in WB

Western blot was performed using Anti-Cytochrome C Monoclonal Antibody (7H8.2C12) (Product # 33-8500) and a ~15kDa band corresponding to Cytochrome C, along with an characterized band(*) was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), SK-O-V3 (Lane 2) and PC-3 (Lane 3) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001... View More

Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytochrome C Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

33-8500

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Fruit fly, Horse, Human, Mouse, Rabbit, Rat, Xenopus

Published species

Chicken, Fish, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

7H8.2C12

Immunogen

Equine Cytochrome c.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533142

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effect of immune modulation on the skeletal muscle mitochondrial exercise response: An exploratory study in mice with cancer.

PloS one

Buss LA,Hock B,Merry TL,Ang AD,Robinson BA,Currie MJ,Dachs GU

Published figure using Cytochrome C monoclonal antibody (Product # 33-8500) in Western Blot

Wed Nov 24 00:00:00 EST 2021

Impact of Exercise and Detraining during Childhood on Brown Adipose Tissue Whitening in Obesity.

Metabolites

Takaishi K,Oshima T,Eto H,Nishihira M,Nguyen ST,Ochi R,Fujita N,Urakawa S

Published figure using Cytochrome C monoclonal antibody (Product # 33-8500) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

The Effects of Potassium Cyanide on the Functional Recovery of Isolated Rat Hearts after Ischemia and Reperfusion: The Role of Oxidative Stress.

Oxidative medicine and cellular longevity

Petkovic AM,Jakovljevic VL,Bradic JV,Jeremic JN,Jeremic NS,Nikolic Turnic TR,Jovicic NU,Rosic VZ,Srejovic IM,Zivkovic VI

Published figure using Cytochrome C monoclonal antibody (Product # 33-8500) in Immunohistochemistry

Wed Dec 19 00:00:00 EST 2018

Tumor protein Tctp regulates axon development in the embryonic visual system.

Development (Cambridge, England)

Roque CG,Wong HH,Lin JQ,Holt CE

33-8500 was used in western blot to assess regulation of axon development in the embryonic visual system by tumor protein Tctp

Fri Apr 01 00:00:00 EDT 2016

Morphine stimulates nitric oxide release in human mitochondria.

Journal of bioenergetics and biomembranes

Stefano GB,Mantione KJ,Capellan L,Casares FM,Challenger S,Ramin R,Samuel JM,Snyder C,Kream RM

33-8500 was used in western blot to discuss morphinergic/NO-coupled regulation.

Thu Oct 01 00:00:00 EDT 2015

The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release.

The Journal of biological chemistry

Hedgepeth SC,Garcia MI,Wagner LE,Rodriguez AM,Chintapalli SV,Snyder RR,Hankins GD,Henderson BR,Brodie KM,Yule DI,van Rossum DB,Boehning D

33-8500 was used in immunoprecipitation to study the interaction between BRCA1 tumor suppressor and inositol 1,4,5-trisphosphate receptors and its effect on apoptotic calcium release.

Fri Mar 13 00:00:00 EDT 2015

The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release.

The Journal of biological chemistry

Hedgepeth SC,Garcia MI,Wagner LE,Rodriguez AM,Chintapalli SV,Snyder RR,Hankins GD,Henderson BR,Brodie KM,Yule DI,van Rossum DB,Boehning D

33-8500 was used in immunoprecipitation to study the interaction between BRCA1 tumor suppressor and inositol 1,4,5-trisphosphate receptors and its effect on apoptotic calcium release.

Fri Mar 13 00:00:00 EDT 2015

Targeting the GD3 acetylation pathway selectively induces apoptosis in glioblastoma.

Neuro-oncology

Birks SM,Danquah JO,King L,Vlasak R,Gorecki DC,Pilkington GJ

Thu Sep 01 00:00:00 EDT 2011

Protective effect of sulforaphane against cisplatin-induced mitochondrial alterations and impairment in the activity of NAD(P)H: quinone oxidoreductase 1 and γ glutamyl cysteine ligase: studies in mitochondria isolated from rat kidney and in LLC-PK1 cells.

Toxicology letters

Guerrero-Beltrán CE,Calderón-Oliver M,Martínez-Abundis E,Tapia E,Zarco-Márquez G,Zazueta C,Pedraza-Chaverri J

33-8500 was used in western blot to test if sulforaphane alters cisplatin-induced mitochondrial alterations and impairs cytoprotective enzyme activity

Wed Nov 10 00:00:00 EST 2010

Lindane and cell death: at the crossroads between apoptosis, necrosis and autophagy.

Toxicology

Zucchini-Pascal N,de Sousa G,Rahmani R

33-8500 was used in western blot to examine the effects of lindane on autophagic, apoptotic, and necrotic cell death in rat hepatocytes

Wed Feb 04 00:00:00 EST 2009

Mitochondrial disruption in Drosophila apoptosis.

Developmental cell

Abdelwahid E,Yokokura T,Krieser RJ,Balasundaram S,Fowle WH,White K

33-8500 was used in immunohistochemistry and western blot to study how Reaper, Hid, and Drp1 regulate mitochondrial disruption in fly apoptosis

Tue May 01 00:00:00 EDT 2007

Mitochondrial impairment induced by poly(ADP-ribose) polymerase-1 activation in cortical neurons after oxygen and glucose deprivation.

Journal of neurochemistry

Tanaka S,Takehashi M,Iida S,Kitajima T,Kamanaka Y,Stedeford T,Banasik M,Ueda K

33-8500 was used in western blot to report that PARP-1 contributes to mitochondrial impairment that ultimately leads to neuronal apoptosis after cerebral ischemia.

Sat Oct 01 00:00:00 EDT 2005

Cadmium-induced apoptosis through the mitochondrial pathway in rainbow trout hepatocytes: involvement of oxidative stress.

Aquatic toxicology (Amsterdam, Netherlands)

Risso-de Faverney C,Orsini N,de Sousa G,Rahmani R

Wed Aug 25 00:00:00 EDT 2004

Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis.

The Journal of biological chemistry

Reiss U,Oskouian B,Zhou J,Gupta V,Sooriyakumaran P,Kelly S,Wang E,Merrill AH,Saba JD

Fri Jan 09 00:00:00 EST 2004

Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.

Journal of cell science

Love DC,Kochan J,Cathey RL,Shin SH,Hanover JA

Sat Feb 15 00:00:00 EST 2003

Mycobacterium tuberculosis virulence correlates with mitochondrial cytochrome c release in infected macrophages.

Scandinavian journal of immunology

Abarca-Rojano E,Rosas-Medina P,Zamudio-Cortéz P,Mondragón-Flores R,Sánchez-García FJ

33-8500 was used in western blot to examine mitochondrial function in the course of intracellular infections.

Wed Oct 01 00:00:00 EDT 2003

Afferent regulation of cytochrome-c and active caspase-9 in the avian cochlear nucleus.

Neuroscience

Wilkinson BL,Elam JS,Fadool DA,Hyson RL

33-8500 was used in Western Blotting to examine the extrusion of cytochrome-c from the mitochondria into the cytosol and the subsequent activation of proteolytic caspase cascades in the nucleus magnocellularis following deafferentation.

Mon Nov 10 00:00:00 EST 2003

Afferent regulation of cytochrome-c and active caspase-9 in the avian cochlear nucleus.

Neuroscience

Wilkinson BL,Elam JS,Fadool DA,Hyson RL

Mon Nov 10 00:00:00 EST 2003

Microcin E492, a channel-forming bacteriocin from Klebsiella pneumoniae, induces apoptosis in some human cell lines.

Proceedings of the National Academy of Sciences of the United States of America

Hetz C,Bono MR,Barros LF,Lagos R

Published figure using Cytochrome C monoclonal antibody (Product # 33-8500) in Western Blot

Tue Mar 05 00:00:00 EST 2002

Microcin E492, a channel-forming bacteriocin from Klebsiella pneumoniae, induces apoptosis in some human cell lines.

Proceedings of the National Academy of Sciences of the United States of America

Hetz C,Bono MR,Barros LF,Lagos R

Published figure using Cytochrome C monoclonal antibody (Product # 33-8500) in Western Blot

Tue Mar 05 00:00:00 EST 2002

The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space.

FEBS letters

Olichon A,Emorine LJ,Descoins E,Pelloquin L,Brichese L,Gas N,Guillou E,Delettre C,Valette A,Hamel CP,Ducommun B,Lenaers G,Belenguer P

33-8500 was used in western blot to report that OPA1 resides in the mitochondria.

Wed Jul 17 00:00:00 EDT 2002

Galectin-3 translocates to the perinuclear membranes and inhibits cytochrome c release from the mitochondria. A role for synexin in galectin-3 translocation.

The Journal of biological chemistry

Yu F,Finley RL,Raz A,Kim HR

Fri May 03 00:00:00 EDT 2002

Galectin-3 translocates to the perinuclear membranes and inhibits cytochrome c release from the mitochondria. A role for synexin in galectin-3 translocation.

The Journal of biological chemistry

Yu F,Finley RL,Raz A,Kim HR

Fri May 03 00:00:00 EDT 2002

Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism.

The Journal of biological chemistry

Tang D,Lahti JM,Grenet J,Kidd VJ

33-8500 was used in western blot to elucidate the mechanisms that regulate cycloheximide-induced apoptosis

Fri Mar 12 00:00:00 EST 1999

Bioinformatics

Protein Aliases: CG17903-PA; CG17903-PB; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p

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Gene Aliases: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4

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UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029

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Entrez Gene ID: (Human) 54205, (Horse) 100053958, (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996, (Rabbit) 100341175, (Rabbit) 100357840

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Function(s)

protein serine/threonine phosphatase activity protein binding heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding electron carrier activity iron ion binding lipid binding

Process(es)

response to reactive oxygen species mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic DNA fragmentation apoptotic process hydrogen peroxide metabolic process ATP synthesis coupled electron transport oxidative phosphorylation positive regulation of cysteine-type endopeptidase activity involved in apoptotic process oxidation-reduction process response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytochrome C Monoclonal Antibody (7H8.2C12)

Cytochrome C Antibody (33-8500) in WB

Product Details

33-8500

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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