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Cytochrome C Antibody (459160) in WB
Western blot analysis of Cytochrome C in Jurkat, 143B, and HeLa cells using a Cytocrome C Monoclonal antibody (Product # 459160). Apoptosis was induced in Jurkat, 143B osteosarcoma, and HeLa cells bia treatment with FAS and staurosporine. Isolation of Mitochondrial and cytoplasmic fractions were performed before detecting with the Cyctocrome C Monoclonal antibody (Product # 459160). The western blot shows that Cytochrome c has translocated partially in FAS-induced cells and STS-treated 43B osteosarcoma cells, and almost completely in STS-t... View More
Product Details
459160
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ Ig
Class
Cocktail
Type
Antibody
Clone
Cocktail
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
IgG fraction
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
RRID
AB_2532229
Product Specific Information
ApoTrack™ Cytochrome c WB Cocktail (Sku# 459160) provides a means to detect apoptosis while detecting release of cytochrome c, as well as a cytoplasmic and mitochondrial markers with the help of western blot. This cocktail provides a means to detect cytochrome c, as well as a cytoplasmic and mitochondrial markers
Antibodies Cocktail Contains:
Mouse anti Cyt. c monoclonal:
Amount: 50 µg
Working concentration: 1 µg/mL
Mouse anti GAPDH monclonal:
Amount: 5 µg
Working concentration: 0.1 µg/mL
Mouse anti PDH-E1-alpha monoclonal:
Amount: 100 µg
Working concentration: 2 µg/mL
Mouse anti C-V-alpha monoclonal:
Amount: 25 µg
Working concentration: 0.5 µg/mL
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytochrome c; hc1
Gene Aliases: CYC; CYCS; HCS; THC4
UniProt ID: (Human) P99999
Entrez Gene ID: (Human) 54205
response to reactive oxygen species
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
protein dephosphorylation
mitochondrion organization
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cellular respiration
intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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