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Invitrogen
Cytochrome P450 Reductase Monoclonal Antibody (OTI6C4)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 12
Cytochrome P450 Reductase Antibody (MA5-24964) in ICC/IF
Immunofluorescence analysis of NADPH-cytochrome P450 reductase was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytochrome P450 Reductase Monoclonal Antibody (OTI6C4) (Product # MA5-24964) at 1:100 d... View More
Product Details
MA5-24964
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI6C4
Immunogen
Full length human recombinant protein of POR produced in HEK293T cell
Conjugate
Form
Liquid
Concentration
0.74 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2724188
Target Information
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCR; CPR; CY DKFZp686G04235; cytochrome P450 reductase; DKFZp686G04235; FLJ26468; NADPH Cytochrome; NADPH--cytochrome P450 reductase; NADPH-dependent cytochrome P450 reductase; P450 (cytochrome) oxidoreductase; P450 Reductase; P450R
Gene Aliases: 4933424M13Rik; CPR; CYPOR; P450R; POR
UniProt ID: (Human) P16435, (Rat) P00388, (Mouse) P37040
Entrez Gene ID: (Human) 5447, (Rat) 29441, (Mouse) 18984
NADPH-hemoprotein reductase activity
cytochrome-b5 reductase activity, acting on NAD(P)H
protein binding
nitric oxide dioxygenase activity
electron carrier activity
FMN binding
hydrolase activity
enzyme binding
[methionine synthase] reductase activity
iron-cytochrome-c reductase activity
flavin adenine dinucleotide binding
NADP binding
iron ion binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
oxidoreductase
regulation of growth plate cartilage chondrocyte proliferation
xenobiotic metabolic process
response to nutrient
carnitine metabolic process
flavonoid metabolic process
internal peptidyl-lysine acetylation
fatty acid oxidation
positive regulation of chondrocyte differentiation
positive regulation of monooxygenase activity
response to drug
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
nitrate catabolic process
positive regulation of cholesterol biosynthetic process
positive regulation of smoothened signaling pathway
nitric oxide catabolic process
oxidation-reduction process
negative regulation of lipase activity
demethylation
cellular response to follicle-stimulating hormone stimulus
cellular response to peptide hormone stimulus
positive regulation of steroid hormone biosynthetic process
cellular organofluorine metabolic process
negative regulation of apoptotic process
cellular response to gonadotropin stimulus
regulation of cholesterol metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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