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Cytokeratin 10 Antibody (PA5-104456) in IHC (P)
Immunohistochemistry analysis of Cytokeratin 10 in human esophageal cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Cytokeratin 10 polyclonal antibody (Product # PA5-104456) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-104456
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KRT10(Accession P13645), corresponding to amino acid residues G140-Q190.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853757
Product Specific Information
Antibody detects endogenous levels of total Cytokeratin 10.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e., skin) and non-keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8. 5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 56 kDa cytokeratin; CK-10; cytokeratin 10; Cytokeratin-10; cytokeratin-17; epidermal keratin 10; intermediate filament protein; K10; keratin 10, type I; keratin complex 1, acidic, gene 10; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 59 kDa; Keratin-10; keratin-17; krt10 {ECO:0000250|UniProtKB:P13645}; suprabasal cytokeratin 10; type I keratin KA10
Gene Aliases: BCIE; BIE; CK10; D130054E02Rik; EHK; K10; K1C1; Ka10; KPP; Krt-1.10; Krt1-10; KRT10
UniProt ID: (Human) P13645, (Mouse) P02535
Entrez Gene ID: (Human) 3858, (Mouse) 16661, (Rat) 450225
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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