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Invitrogen
Cytokeratin 14 Polyclonal Antibody
FIGURE: 1 / 4
Cytokeratin 14 Antibody (PA5-28002) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Cytokeratin 14 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 Polyclonal Antibody (Product # PA5 28002) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-28002
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 112 and 472 of Human Cytokeratin 14
Conjugate
Form
Liquid
Concentration
0.48 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545478
Product Specific Information
Recommended positive controls: HeLa, rat olfactory epithelium.
Predicted reactivity: Mouse (95%), Rat (94%), Dog (96%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). At least 20 different CKs can be identified. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Belonging to the type I subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CK-14; cytokeratin 14; Cytokeratin-14; K14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin complex 1, acidic, gene 14; Keratin, type I cytoskeletal 14; Keratin-14; Koebner; Type I keratin Ka14
Gene Aliases: CK14; EBS3; EBS4; K14; Ka14; Krt1-14; KRT14; NFJ
UniProt ID: (Human) P02533, (Rat) Q6IFV1
Entrez Gene ID: (Human) 3861, (Rat) 287701
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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