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Invitrogen
Cytokeratin 17 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Cytokeratin 17 Antibody (PA5-29919) in ICC/IF
Immunofluorescence analysis of Cytokeratin 17 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 17 Polyclonal Antibody (Product # PA5-29919) at 1:100 dilution in 0.1% BSA and incubated overnig... View More
Product Details
PA5-29919
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 59 and 291 of Human Cytokeratin 17
Conjugate
Form
Liquid
Concentration
0.29 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547393
Product Specific Information
Recommended positive controls: HeLa.
Predicted reactivity: Mouse (94%), Rat (94%), Sheep (92%), Chimpanzee (100%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately40 kDa and approximately68 kDa and isoelectric pH between 4. 9 - 7. 8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 39.1; CK-17; CK17; Cytokeratin-17; hard keratin; K17; keratin 17, type I; Keratin, type I cytoskeletal 17; Keratin-17
Gene Aliases: 39.1; CK-17; K17; KRT17; PC; PC2; PCHC1
UniProt ID: (Human) Q04695
Entrez Gene ID: (Human) 3872
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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