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Invitrogen
Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody (ARC2303)
FIGURE: 1 / 7
Cytokeratin 18 Antibody (MA5-37993) in IHC (P)
Immunohistochemistry analysis of Cytokeratin 18 in paraffin-embedded human liver cancer. Samples were incubated with Cytokeratin 18 Monoclonal antibody (Product # MA5-37993) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product Details
MA5-37993
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2303
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-430 of human Cytokeratin 18 (KRT18) (P05783)
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897911
Product Specific Information
Positive Samples: HeLa, HepG2, A-431
Immunogen sequence: ADIRAQYDEL ARKNREELDK YWSQQIEEST TVVTTQSAEV GAAETTLTEL RRTVQSLEID LDSMRNLKAS LENSLREVEA RYALQMEQLN GILLHLESEL AQTRAEGQRQ AQEYEALLNI KVKLEAEIAT YRRLLEDGED FNLGDALDSS NSMQTIQKTT TRRIVDGKVV SETNDTKVLR H
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; Endo B; K 18; K18; keratin 18, type I; keratin complex 1, acidic, gene 18; Keratin D; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; krt18 {ECO:0000250|UniProtKB:P05783}; type I cytoskeletal 18
Gene Aliases: CK-18; CK18; CYK18; K18; Kerd; Krt1-18; KRT18; PIG46
UniProt ID: (Human) P05783, (Rat) Q5BJY9, (Mouse) P05784
Entrez Gene ID: (Human) 3875, (Rat) 294853, (Mouse) 16668
cell cycle
anatomical structure morphogenesis
viral process
tumor necrosis factor-mediated signaling pathway
Golgi to plasma membrane CFTR protein transport
negative regulation of apoptotic process
intermediate filament cytoskeleton organization
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
cell-cell adhesion
biological_process
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