Invitrogen

Cytokeratin 18 Monoclonal Antibody (DC-10), FITC

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (1)

Cytokeratin 18 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

Cytokeratin 18 Antibody (MA1-10327) in Flow

Flow cytometry analysis (intracellular staining) of HeLa cells with anti-cytokeratin 18 (DC-10) FITC Monoclonal antibody (Product # MA1-10327).

Product Image Gallery

Product Details

MA1-10327

Applications

Tested Dilution

Publications

Western Blot (WB)

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Flow Cytometry (Flow)

1-5 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DC-10

Immunogen

Human breast carcinoma cell line PMC-42.

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154144

Product Specific Information

This antibody reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.

This antibody will not cross-react with porcine, mouse, rat, hamster, bovine, canine or sheep.

Target Information

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

MA1-10327 was used in Flow cytometry/Cell sorting to provide a foundation with which to understand the fate of human breast milk cells in the infant's intestine and beyond.

Sat Oct 01 00:00:00 EDT 2022

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

MA1-10327 was used in Flow cytometry/Cell sorting to provide a foundation with which to understand the fate of human breast milk cells in the infant's intestine and beyond.

Sat Oct 01 00:00:00 EDT 2022

Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.

The Journal of cell biology

Snider NT,Leonard JM,Kwan R,Griggs NW,Rui L,Omary MB

MA1-10327 was used in immunoprecipitation to study the opposing effects of hyperglycemia and SIRT2 on keratin-8 lysine acetylation

Mon Feb 04 00:00:00 EST 2013

Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Marian C,Boila A,Soanca D,Malau M,Podeanu DM,Resetkova E,Stolnicu S

MA1-10327 was used in immunohistochemistry to report on two cases of malignant transformation of adenomyoepithelioma of the breast by a monophasic population

Mon Apr 01 00:00:00 EDT 2013

Expression of KRT7 and WT1 differentiates precursor lesions of Wilms' tumours from those of papillary renal cell tumours and mucinous tubular and spindle cell carcinomas.

Virchows Archiv : an international journal of pathology

Szponar A,Kovacs G

MA1-10327 was used in immunohistochemistry to study the value of KRT7 and WT1 expression profiles in differentiating Wilms' tumour, papillary renal cell tumour and mucinous tubular and spindle cell carcinoma precursor lesions

Sun Apr 01 00:00:00 EDT 2012

Cytoskeletal keratin glycosylation protects epithelial tissue from injury.

Nature cell biology

Ku NO,Toivola DM,Strnad P,Omary MB

MA1-10327 was used in western blot to study the role of cytoskeletal keratin glycosylation in protecting epithelial tissue from injury

Wed Sep 01 00:00:00 EDT 2010

Endometrial profile of tamoxifen and low-dose estradiol combination therapy.

Clinical cancer research : an official journal of the American Association for Cancer Research

Wood CE,Kaplan JR,Fontenot MB,Williams JK,Cline JM

MA1-10327 was used in immunohistochemistry to detect effects of estradiol and tamoxifen on endometrial health

Mon Feb 01 00:00:00 EST 2010

Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates.

Cell death and differentiation

Yalcin-Ozuysal O,Fiche M,Guitierrez M,Wagner KU,Raffoul W,Brisken C

MA1-10327 was used in western blot to investigate the role of Notch and p63 in regulation of mammary epithelial cell fates

Fri Oct 01 00:00:00 EDT 2010

Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.

Research in veterinary science

Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS

MA1-10327 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines

Thu Apr 01 00:00:00 EDT 2010

Giant clear cell hidradenoma of the knee.

Journal of cutaneous pathology

Yu G,Goodloe S,D'Angelis CA,McGrath BE,Chen F

MA1-10327 was used in immunohistochemistry to report on a case of giant clear cell hidradenoma of the knee

Wed Sep 01 00:00:00 EDT 2010

Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.

Journal of biomolecular screening

Robertson FM,Ogasawara MA,Ye Z,Chu K,Pickei R,Debeb BG,Woodward WA,Hittelman WN,Cristofanilli M,Barsky SH

MA1-10327 was used in immunohistochemistry to study 3D tumor spheroids enriched for a cancer stem cell phenotype

Sun Aug 01 00:00:00 EDT 2010

Directed differentiation of human embryonic stem cells to epidermal progenitors.

Methods in molecular biology (Clifton, N.J.)

Metallo CM,Ji L,de Pablo JJ,Palecek SP

MA1-10327 was used in flow cytometry to discus a method for the differentiation of human embryonic stem cells into epidermal progenitors

Fri Jan 29 00:00:00 EST 2010

Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation.

Cancer research

Røsland GV,Svendsen A,Torsvik A,Sobala E,McCormack E,Immervoll H,Mysliwietz J,Tonn JC,Goldbrunner R,Lønning PE,Bjerkvig R,Schichor C

MA1-10327 was used in immunohistochemistry to study malignant transformation in long-term cultures of bone marrow-derived human mesenchymal stem cells

Wed Jul 01 00:00:00 EDT 2009

Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation.

The Journal of pathology

Tao GZ,Li DH,Zhou Q,Toivola DM,Strnad P,Sandesara N,Cheung RC,Hong A,Omary MB

MA1-10327 was used in ELISA to study epithelial cell caspase activation via detection of a durable keratin fragment

Sun Jun 01 00:00:00 EDT 2008

Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association

Tao GZ,Strnad P,Zhou Q,Kamal A,Zhang L,Madani ND,Kugathasan S,Brant SR,Cho JH,Omary MB,Duerr RH

MA1-10327 was used in western blot to study whether variants of keratin 8 and keratin 19 are associated with inflammatory bowel disease

Sun Jul 01 00:00:00 EDT 2007

An immunohistochemical profile of giant cell carcinoma of the larynx.

Auris, nasus, larynx

Gurbuz Y,Kose N,Aydin O,Ozturk M

MA1-10327 was used in immunohistochemistry to report on a case of giant cell carcinoma of the larynx

Sat Sep 01 00:00:00 EDT 2007

Mammary gland development in early pubertal female macaques.

Toxicologic pathology

Wood CE,Hester JM,Cline JM

MA1-10327 was used in immunohistochemistry to investigate the effect of estradiol on early ductal and lobular development

Mon Oct 01 00:00:00 EDT 2007

Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner.

Journal of cell science

Tao GZ,Toivola DM,Zhou Q,Strnad P,Xu B,Michie SA,Omary MB

MA1-10327 was used in western blot to study the site-specific dephosphorylation of keratin 8 by protein phosphatase-2A and how this varies according to cell type

Sat Apr 01 00:00:00 EST 2006

Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease.

Hepatology (Baltimore, Md.)

Toivola DM,Ku NO,Resurreccion EZ,Nelson DR,Wright TL,Omary MB

MA1-10327 was used in western blot to study keratin 8 and keratin 18 hyperphosphorylation as markers for progression of human liver disease

Sun Aug 01 00:00:00 EDT 2004

Cytokeratin expression in lichen amyloidosus and macular amyloidosis.

Journal of the European Academy of Dermatology and Venereology : JEADV

Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N

MA1-10327 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value

Sat May 01 00:00:00 EDT 2004

Keratin mutation in transgenic mice predisposes to Fas but not TNF-induced apoptosis and massive liver injury.

Hepatology (Baltimore, Md.)

Ku NO,Soetikno RM,Omary MB

MA1-10327 was used in western blot to study the role of keratin mutations in the predisposition to Fas-induced apoptosis and massive liver injury

Thu May 01 00:00:00 EDT 2003

Keratin 8 mutations in patients with cryptogenic liver disease.

The New England journal of medicine

Ku NO,Gish R,Wright TL,Omary MB

MA1-10327 was used in western blot to study mutations of keratin 8 in cryptogenic liver disease patients

Thu May 24 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin-18; Cytokeratin18; K 18; K18; keratin 18, type I; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; type I cytoskeletal 18

View more View less

Gene Aliases: CK-18; CYK18; K18; KRT18; PIG46

View more View less

UniProt ID: (Human) P05783

View more View less

Entrez Gene ID: (Human) 3875

View more View less

Function(s)

structural molecule activity protein binding poly(A) RNA binding scaffold protein binding cadherin binding involved in cell-cell adhesion

Process(es)

cell cycle anatomical structure morphogenesis viral process tumor necrosis factor-mediated signaling pathway Golgi to plasma membrane CFTR protein transport negative regulation of apoptotic process intermediate filament cytoskeleton organization extrinsic apoptotic signaling pathway hepatocyte apoptotic process cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Cytokeratin 18 Monoclonal Antibody (DC-10), FITC

Cytokeratin 18 Antibody (MA1-10327) in Flow

Product Details

MA1-10327

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!