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Invitrogen
Cytokeratin 18 Monoclonal Antibody (RGE53)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
Cytokeratin 18 Antibody (MA1-06326) in ICC/IF
Immunofluorescence analysis of Cytokeratin 18 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 18 Monoclonal Antibody (RGE53) (Product # MA1-06326) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled w... View More
Product Details
MA1-06326
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Hamster,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Zebrafish
Published species
Mouse,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
RGE53
Immunogen
Cytoskeletal preparation of HeLa cells
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_559772
Product Specific Information
MA1-06326 detects cytokeratin 18 in human, mouse, rat, hamster, rabbit, chicken, canine, zebrafish and swine samples.
MA1-06326 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry, and immunohistochemistry.
The MA1-06326 immunogen is a cytoskeletal preparation from cells.
Store at 4ºC or in small aliquots at -20ºC.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin endo B; cytokeratin type -1; DAPK-1; Cytokeratin-18; Cytokeratin18; Dorsal aorta proneprin kinesin-1; DreK18; Endo B; K 18; K18; keratin 18; keratin 18, type I; keratin complex 1, acidic, gene 18; Keratin D; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; krt18 {ECO:0000250|UniProtKB:P05783}; type I cytoskeletal 18
Gene Aliases: cb83; CK-18; CK18; CYK18; dapk1; DreK18; I79_023185; K18; Kerd; Krt1-18; KRT18; PIG46; sb:cb83; wu:fa13f02; wu:fb36b04
UniProt ID: (Human) P05783, (Rat) Q5BJY9, (Mouse) P05784, (Zebrafish) Q7ZTS4
cell cycle
anatomical structure morphogenesis
viral process
tumor necrosis factor-mediated signaling pathway
Golgi to plasma membrane CFTR protein transport
negative regulation of apoptotic process
intermediate filament cytoskeleton organization
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
cell-cell adhesion
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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