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Invitrogen
Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody (HL1165)
FIGURE: 1 / 4
Cytokeratin 19 Antibody (MA5-46945) in ICC/IF
Immunofluorescent analysis of Cytokeratin 19 in MCF-7 cells. Cells were fixed in ice-cold MeOH for 10 min. Green: Cytokeratin 19 stained by Cytokeratin 19 monoclonal antibody (Product # MA5-46945) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI Scale bar= 10 µm.
Product Details
MA5-46945
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL1165
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cytokeratin 19. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2938017
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Target Information
Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40-kDa keratin intermediate filament; C K1CS; CK 19; CK-19; cytokeratin 19; Cytokeratin-19; K19; keratin 19, type I; keratin complex 1, acidic, gene 19; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin-19; MGC15366; Type I keratin Ka19
Gene Aliases: AI663979; CK19; EndoC; K19; K1CS; Ka19; Krt-1.19; Krt1-19; KRT19
UniProt ID: (Human) P08727, (Mouse) P19001, (Rat) Q63279
Entrez Gene ID: (Human) 3880, (Mouse) 16669, (Rat) 360626
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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