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Cytokeratin 4 Antibody (MA5-55839) in WB
Western blot analysis of Cytokeratin 4 in 15 µg of A-431. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Cytokeratin 4 band was visualized using ECL Substrate. Incubation with primary Cytokeratin 4 monoclonal antibody (Product # MA5-55839) at a dilution of 1 µg/mL was used.
Product Details
MA5-55839
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
K06324_3B6
Immunogen
Human KRT4 peptide (immunogen range is 511-520).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Sequence of this protein is as follows: ISTTTLNKRR
Target Information
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately40 kDa and approximately68 kDa and isoelectric pH between 4. 9-7. 8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CK-4; cytokeratin 4; Cytokeratin-4; K4; keratin 4, type II; Keratin, type II cytoskeletal 4; Keratin-4; Type-II keratin Kb4
Gene Aliases: CK-4; CK4; CYK4; K4; KRT4; WSN1
UniProt ID: (Human) P19013
Entrez Gene ID: (Human) 3851
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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