Invitrogen

Cytokeratin 5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

10 Published Figures

18 References

View all (82) Cytokeratin 5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Cytokeratin 5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

Cytokeratin 5 Antibody (PA5-32465) in ICC/IF

Immunofluorescence analysis of Cytokeratin 5 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 5 Polyclonal Antibody (Product # PA5-32465) at 1:100 dilution in 0.1% BSA, incubated at 4 de... View More

Cytokeratin 5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytokeratin 5 Antibody in Western Blot (WB)

Cytokeratin 5 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Cytokeratin 5 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-32465

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to C-terminus of human cytokeratin 5.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2549934

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, breast, prostate or mesothelioma.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MIR503HG silencing promotes endometrial stromal cell progression and metastasis and suppresses apoptosis in adenomyosis by activating the Wnt/β‑catenin pathway via targeting miR‑191.

Experimental and therapeutic medicine

Xu X,Cai B,Liu Y,Liu R,Li J

PA5-32465 was used in Immunocytochemistry to investigate the specific role of MIR503HG in adenomyosis pathogenesis and progression using endometrial stromal cells derived from the endometrium of patients with adenomyosis as a model.

Wed Mar 01 00:00:00 EST 2023

Assessment of Beta-2 Microglobulin Gene Edited Airway Epithelial Stem Cells as a treatment for Sulfur Mustard Inhalation.

Frontiers in genome editing

Naeimi Kararoudi M,Alsudayri A,Hill CL,Elmas E,Sezgin Y,Thakkar A,Hester ME,Malleske DT,Lee DA,Neal ML,Perry MR,Harvilchuck JA,Reynolds SD

PA5-32465 was used in Immunohistochemistry to generate B2M-knockout tracheobronchial airway epithelial stem cell , which are termed Universal Donor Stem Cells (UDSC), and characterise them.

Fri Feb 25 00:00:00 EST 2022

A simple method to generate human airway epithelial organoids with externally orientated apical membranes.

American journal of physiology. Lung cellular and molecular physiology

Boecking CA,Walentek P,Zlock LT,Sun DI,Wolters PJ,Ishikawa H,Jin BJ,Haggie PM,Marshall WF,Verkman AS,Finkbeiner WE

PA1-37974 was used in Immunocytochemistry-immunofluorescence to describe a simple method to generate organoid cultures using isolated human tracheobronchial epithelial cells grown in mixed matrix components and supplemented at day 14 with the Wnt pathway agonist R-spondin 2 (RSPO2) and the bone morphogenic protein antagonist Noggin.

Tue Mar 01 00:00:00 EST 2022

Cigarette Smoke Activates NOTCH3 to Promote Goblet Cell Differentiation in Human Airway Epithelial Cells.

American journal of respiratory cell and molecular biology

Bodas M,Moore AR,Subramaniyan B,Georgescu C,Wren JD,Freeman WM,Brown BR,Metcalf JP,Walters MS

PA1-37974 was used in Immunohistochemistry (Paraffin) to identify NOTCH3 activation as one of the important mechanisms by which cigarette smoke induces goblet cell differentiation, thus providing a novel potential strategy to control GCMH-related pathologies in smokers and patients with COPD.

Thu Apr 01 00:00:00 EDT 2021

The NOTCH3 Downstream Target HEYL Is Required for Efficient Human Airway Basal Cell Differentiation.

Cells

Bodas M,Subramaniyan B,Moore AR,Metcalf JP,Ocañas SR,Freeman WM,Georgescu C,Wren JD,Walters MS

PA1-37974 was used in Immunocytochemistry to investigate the mechanisms of NOTCH3 receptor signaling regulating basal cells to club cell differentiation.

Thu Nov 18 00:00:00 EST 2021

Role of KRAS in regulating normal human airway basal cell differentiation.

Respiratory research

Ogawa F,Walters MS,Shafquat A,O'Beirne SL,Kaner RJ,Mezey JG,Zhang H,Leopold PL,Crystal RG

Published figure using Cytokeratin 5 polyclonal antibody (Product # PA5-32465) in Immunofluorescence

Fri Aug 09 00:00:00 EDT 2019

Role of KRAS in regulating normal human airway basal cell differentiation.

Respiratory research

Ogawa F,Walters MS,Shafquat A,O'Beirne SL,Kaner RJ,Mezey JG,Zhang H,Leopold PL,Crystal RG

Published figure using Cytokeratin 5 polyclonal antibody (Product # PA5-32465) in Immunofluorescence

Fri Aug 09 00:00:00 EDT 2019

ΔN-Tp63 Mediates Wnt/β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia.

Cell reports

Haas M,Gómez Vázquez JL,Sun DI,Tran HT,Brislinger M,Tasca A,Shomroni O,Vleminckx K,Walentek P

Published figure using Cytokeratin 5 polyclonal antibody (Product # PA5-32465) in Immunocytochemistry

Tue Sep 24 00:00:00 EDT 2019

Characterization of an immortalized human small airway basal stem/progenitor cell line with airway region-specific differentiation capacity.

Respiratory research

Wang G,Lou HH,Salit J,Leopold PL,Driscoll S,Schymeinsky J,Quast K,Visvanathan S,Fine JS,Thomas MJ,Crystal RG

PA5-32465 was used in Immunocytochemistry-immunoflourescence to generate and characterise an immortalized human small airway basal cell line.

Fri Aug 23 00:00:00 EDT 2019

Na+/H+ Exchangers Are Required for the Development and Function of Vertebrate Mucociliary Epithelia.

Cells, tissues, organs

Sun DI,Tasca A,Haas M,Baltazar G,Harland RM,Finkbeiner WE,Walentek P

PA1-37974 was used in Immunohistochemistry to study the contribution of Na+/H+ exchangers to signalling, development and pathogenesis in the human respiratory tract.

Mon Aug 26 00:00:00 EDT 2019

Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration.

Oncotarget

Zhang H,Yang J,Walters MS,Staudt MR,Strulovici-Barel Y,Salit J,Mezey JG,Leopold PL,Crystal RG

Tue Mar 06 00:00:00 EST 2018

Endothelial Cell Mediated Promotion of Ciliated Cell Differentiation of Human Airway Basal Cells via Insulin and Insulin-Like Growth Factor 1 Receptor Mediated Signaling.

Stem cell reviews and reports

Gomi K,Tang Y,Arbelaez V,Crystal RG,Walters MS

PA137974 was used in immunocytochemistry to attempt to understand the role of human airway basal cells and endothelial cells cross-talk in regulating basal cell stem/progenitor function

Sat Apr 01 00:00:00 EDT 2017

Endothelial Cell Mediated Promotion of Ciliated Cell Differentiation of Human Airway Basal Cells via Insulin and Insulin-Like Growth Factor 1 Receptor Mediated Signaling.

Stem cell reviews and reports

Gomi K,Tang Y,Arbelaez V,Crystal RG,Walters MS

PA137974 was used in immunocytochemistry to attempt to understand the role of human airway basal cells and endothelial cells cross-talk in regulating basal cell stem/progenitor function

Sat Apr 01 00:00:00 EDT 2017

JAG1-Mediated Notch Signaling Regulates Secretory Cell Differentiation of the Human Airway Epithelium.

Stem cell reviews and reports

Gomi K,Staudt MR,Salit J,Kaner RJ,Heldrich J,Rogalski AM,Arbelaez V,Crystal RG,Walters MS

PA137974 was used in immunocytochemistry to determine the role of JAG1 human basal cell differentiation

Mon Aug 01 00:00:00 EDT 2016

Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells.

Journal of cell science

Ding BS,Gomi K,Rafii S,Crystal RG,Walters MS

PA1-37974 was used in immunocytochemistry to investigate factors involved in the crosstalk between basal cells and endothelial cells.

Sat Aug 15 00:00:00 EDT 2015

Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway.

PloS one

Gomi K,Arbelaez V,Crystal RG,Walters MS

PA1-37974 was used in immunocytochemistry and western blot to investigate the role of Notch signaling in human basal cell differentiation

Wed Jan 13 00:00:00 EST 2016

Activation of soluble guanylyl cyclase prevents foam cell formation and atherosclerosis.

Acta physiologica (Oxford, England)

Tsou CY,Chen CY,Zhao JF,Su KH,Lee HT,Lin SJ,Shyue SK,Hsiao SH,Lee TS

PA1-37974 was used in western blot to study the mechanism by which soluble guanylyl cyclase activation blocks macrophage foam cell formation and atherosclerosis

Tue Apr 01 00:00:00 EDT 2014

Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity.

Respiratory research

Walters MS,Gomi K,Ashbridge B,Moore MA,Arbelaez V,Heldrich J,Ding BS,Rafii S,Staudt MR,Crystal RG

Published figure using Cytokeratin 5 polyclonal antibody (Product # PA5-32465) in Western Blot

Tue Dec 03 00:00:00 EST 2013

Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity.

Respiratory research

Walters MS,Gomi K,Ashbridge B,Moore MA,Arbelaez V,Heldrich J,Ding BS,Rafii S,Staudt MR,Crystal RG

Published figure using Cytokeratin 5 polyclonal antibody (Product # PA5-32465) in Western Blot

Tue Dec 03 00:00:00 EST 2013

Bioinformatics

Protein Aliases: 58 kDa cytokeratin; CK-5; cytokeratin 5; Cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin complex 2, basic, gene 5; Keratin, type II cytoskeletal 5; Keratin-5; krt5; tuftelin interacting protein 8; Type-II keratin Kb5

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Gene Aliases: 3300001P10Rik; AW146334; CK5; DDD; DDD1; EBS2; K5; Krt2-5; KRT5; KRT5A; Sak57; Tfip8

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UniProt ID: (Human) P13647, (Rat) Q6P6Q2, (Mouse) Q922U2

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Entrez Gene ID: (Human) 3852, (Rat) 369017, (Mouse) 110308

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Function(s)

structural constituent of cytoskeleton protein binding scaffold protein binding molecular_function structural molecule activity

Process(es)

cytoskeleton organization epidermis development hemidesmosome assembly biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin 5 Polyclonal Antibody

Cytokeratin 5 Antibody (PA5-32465) in ICC/IF

Product Details

PA5-32465

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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