Invitrogen

Cytokeratin 5 Monoclonal Antibody (2C2)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

11 References

View all (82) Cytokeratin 5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Cytokeratin 5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

Cytokeratin 5 Antibody (MA5-17057) in ICC/IF

Immunofluorescence analysis of HeLa cells using CK5 monoclonal antibody (Product # MA5-17057). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Cytokeratin 5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytokeratin 5 Antibody in Western Blot (WB)

Cytokeratin 5 Antibody in Flow Cytometry (Flow)

Cytokeratin 5 Antibody in Immunohistochemistry (IHC)

Cytokeratin 5 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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Product Details

MA5-17057

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Cynomolgus monkey, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2C2

Immunogen

Purified recombinant fragment of human CK5 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538529

Product Specific Information

MA5-17057 targets CK5 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples.

The MA5-17057 immunogen is purified recombinant fragment of human CK5 expressed in E. Coli.

MA5-17057 detects CK5 which has a predicted molecular weight of approximately 62.3kDa.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel insights linking BRCA1-IRIS role in mammary gland development to formation of aggressive PABCs: the case for longer breastfeeding.

American journal of cancer research

Castillo P,Aisagbonhi O,Saenz CC,ElShamy WM

MA5-17057 was used in Western Blot to determine the effect of the pregnancy/lactation/involution microenvironments on the expression of IRIS/Iris in normal mammary epithelial cells regarding IRIS/Iris-overexpressing pregnancy-associated breast cancers.

Wed Feb 16 00:00:00 EST 2022

Modelling aggressive prostate cancers of young men in immune-competent mice, driven by isogenic Trp53 alterations and Pten loss.

Cell death & disease

Mejía-Hernández JO,Keam SP,Saleh R,Muntz F,Fox SB,Byrne D,Kogan A,Pang L,Huynh J,Litchfield C,Caramia F,Lozano G,He H,You JM,Sandhu S,Williams SG,Haupt Y,Haupt S

MA5-17057 was used in Immunohistochemistry to support the hypothesis that individual p53 mutations confer their own particular oncogenic gain of function in prostate cancer.

Thu Sep 08 00:00:00 EDT 2022

Modelling aggressive prostate cancers of young men in immune-competent mice, driven by isogenic Trp53 alterations and Pten loss.

Cell death & disease

Mejía-Hernández JO,Keam SP,Saleh R,Muntz F,Fox SB,Byrne D,Kogan A,Pang L,Huynh J,Litchfield C,Caramia F,Lozano G,He H,You JM,Sandhu S,Williams SG,Haupt Y,Haupt S

MA5-17057 was used in Immunohistochemistry to support the hypothesis that individual p53 mutations confer their own particular oncogenic gain of function in prostate cancer.

Thu Sep 08 00:00:00 EDT 2022

Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer.

Scientific reports

Le UQ,Chen N,Balasenthil S,Lurie E,Yang F,Liu S,Rubin L,Solis Soto LM,Raso MG,Batra H,Sahin AA,Wistuba II,Killary AM

MA5-17057 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that DEAR1 maintains luminal differentiation as a novel ubiquitin ligase for SNAI2/SLUG, a master regulator driving stemness and generation of basal-like progenitor populations.

Mon Nov 14 00:00:00 EST 2022

Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer.

Scientific reports

Le UQ,Chen N,Balasenthil S,Lurie E,Yang F,Liu S,Rubin L,Solis Soto LM,Raso MG,Batra H,Sahin AA,Wistuba II,Killary AM

Mon Nov 14 00:00:00 EST 2022

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Immunity

Lou F,Sun Y,Xu Z,Niu L,Wang Z,Deng S,Liu Z,Zhou H,Bai J,Yin Q,Cai X,Sun L,Wang H,Li Q,Wu Z,Chen X,Gu J,Shi YL,Tao W,Ginhoux F,Wang H

MA5-17057 was used in Immunohistochemistry to suggest that urea cycle hyperreactivity and excessive polyamine generation in psoriatic keratinocytes promote self-RNA sensation and PP6 deregulation in keratinocytes is a pivotal event that amplifies the inflammatory circuits in psoriasis.

Tue Jul 14 00:00:00 EDT 2020

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Immunity

Lou F,Sun Y,Xu Z,Niu L,Wang Z,Deng S,Liu Z,Zhou H,Bai J,Yin Q,Cai X,Sun L,Wang H,Li Q,Wu Z,Chen X,Gu J,Shi YL,Tao W,Ginhoux F,Wang H

Tue Jul 14 00:00:00 EDT 2020

The pro- and anti-tumor roles of mesenchymal stem cells toward BRCA1-IRIS-overexpressing TNBC cells.

Breast cancer research : BCR

Ryan D,Paul BT,Koziol J,ElShamy WM

MA5-17057 was used in Immunohistochemistry-immunofluorescence to study the effect of BRCA1-IRIS overexpressing TNBC cells and mesenchymal stem cells on tumor formation and progression.

Wed Apr 24 00:00:00 EDT 2019

Lats1 and Lats2 are required for the maintenance of multipotency in the Müllerian duct mesenchyme.

Development (Cambridge, England)

St-Jean G,Tsoi M,Abedini A,Levasseur A,Rico C,Morin M,Djordjevic B,Miinalainen I,Kaarteenaho R,Paquet M,Gévry N,Boyer A,Vanderhyden B,Boerboom D

MA5-17057 was used in Immunohistochemistry to show that conditional deletion of the key Hippo kinases Lats1 and Lats2 in mouse Müllerian duct mesenchyme cells caused them to adopt the myofibroblast cell fate, resulting in profound reproductive tract developmental defects and sterility.

Fri Oct 18 00:00:00 EDT 2019

Enhanced Tropism of Species B1 Adenoviral-Based Vectors for Primary Human Airway Epithelial Cells.

Molecular therapy. Methods & clinical development

Li N,Cooney AL,Zhang W,Ehrhardt A,Sinn PL

Published figure using Cytokeratin 5 monoclonal antibody (Product # MA5-17057) in Immunofluorescence

Fri Sep 13 00:00:00 EDT 2019

Enhanced Tropism of Species B1 Adenoviral-Based Vectors for Primary Human Airway Epithelial Cells.

Molecular therapy. Methods & clinical development

Li N,Cooney AL,Zhang W,Ehrhardt A,Sinn PL

Published figure using Cytokeratin 5 monoclonal antibody (Product # MA5-17057) in Immunofluorescence

Fri Sep 13 00:00:00 EDT 2019

Recurrent hotspot mutations in HRAS Q61 and PI3K-AKT pathway genes as drivers of breast adenomyoepitheliomas.

Nature communications

Geyer FC,Li A,Papanastasiou AD,Smith A,Selenica P,Burke KA,Edelweiss M,Wen HC,Piscuoglio S,Schultheis AM,Martelotto LG,Pareja F,Kumar R,Brandes A,Fan D,Basili T,Da Cruz Paula A,Lozada JR,Blecua P,Muenst S,Jungbluth AA,Foschini MP,Wen HY,Brogi E,Palazzo J,Rubin BP,Ng CKY,Norton L,Varga Z,Ellis IO,Rakha EA,Chandarlapaty S,Weigelt B,Reis-Filho JS

Published figure using Cytokeratin 5 monoclonal antibody (Product # MA5-17057) in Western Blot

Tue May 08 00:00:00 EDT 2018

Urothelial cell expansion and differentiation are improved by exposure to hypoxia.

Journal of tissue engineering and regenerative medicine

Chabaud S,Saba I,Baratange C,Boiroux B,Leclerc M,Rousseau A,Bouhout S,Bolduc S

Published figure using Cytokeratin 5 monoclonal antibody (Product # MA5-17057) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model.

Scientific reports

Liang Y,Zhu F,Zhang H,Chen D,Zhang X,Gao Q,Li Y

MA5-17057 was used in immunohistochemistry - paraffin section investigate the role of TGF-beta signaling in bladder cancer progression

Tue Jul 05 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 58 kDa cytokeratin; CK-5; cytokeratin 5; Cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin complex 2, basic, gene 5; Keratin, type II cytoskeletal 5; Keratin-5; krt5; tuftelin interacting protein 8; Type-II keratin Kb5

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Gene Aliases: 3300001P10Rik; AW146334; CK5; DDD; DDD1; EBS2; K5; Krt2-5; KRT5; KRT5A; Tfip8

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UniProt ID: (Human) P13647, (Mouse) Q922U2

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Entrez Gene ID: (Human) 3852, (Mouse) 110308

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Function(s)

structural constituent of cytoskeleton protein binding scaffold protein binding structural molecule activity

Process(es)

cytoskeleton organization epidermis development hemidesmosome assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin 5 Monoclonal Antibody (2C2)

Cytokeratin 5 Antibody (MA5-17057) in ICC/IF

Product Details

MA5-17057

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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