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Invitrogen
Cytokeratin 8 Monoclonal Antibody (M20)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
Cytokeratin 8 Antibody (MA1-06318) in ICC/IF
Immunofluorescence analysis of Cytokeratin 8 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 8 Monoclonal Antibody (M20) (Product # MA1-06318) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with ... View More
Product Details
MA1-06318
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rabbit,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M20
Immunogen
Keratin isolated from the human breast carcinoma cell line MCF-7.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_559794
Product Specific Information
MA1-06318 detects cytokeratin 8 in rat, human, and rabbit samples.
MA1-06318 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry and immunohistochemistry (frozen, paraffin).
The MA1-06318 immunogen is keratin isolated from the human breast carcinoma cell line MCF-7.
Store at 4ºC or in small aliquots at -20ºC.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CK-8; Cytokeratin endo A; Cytokeratin-8; K8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8
Gene Aliases: CARD2; CK-8; CK8; CYK8; CYKER; K2C8; K8; KO; Krt2-8; KRT8
UniProt ID: (Human) P05787, (Rat) Q10758
Entrez Gene ID: (Rabbit) 100348891, (Human) 3856, (Rat) 25626
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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