Invitrogen

Cytokeratin 8 Monoclonal Antibody (M20)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

9 References

View all (104) Cytokeratin 8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Cytokeratin 8 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

Cytokeratin 8 Antibody (MA1-06318) in ICC/IF

Immunofluorescence analysis of Cytokeratin 8 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 8 Monoclonal Antibody (M20) (Product # MA1-06318) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with ... View More

Cytokeratin 8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytokeratin 8 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Cytokeratin 8 Antibody in Western Blot (WB)

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Product Details

MA1-06318

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

Immunocytochemistry (ICC/IF)

1:100-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human, Rabbit, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M20

Immunogen

Keratin isolated from the human breast carcinoma cell line MCF-7.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559794

Product Specific Information

MA1-06318 detects cytokeratin 8 in rat, human, and rabbit samples.

MA1-06318 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry and immunohistochemistry (frozen, paraffin).

The MA1-06318 immunogen is keratin isolated from the human breast carcinoma cell line MCF-7.

Store at 4ºC or in small aliquots at -20ºC.

Target Information

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Keratin 8 phosphorylation regulates its transamidation and hepatocyte Mallory-Denk body formation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kwan R,Hanada S,Harada M,Strnad P,Li DH,Omary MB

MA1-06318 was used in western blot to study the role of keratin 8 phosphorylation in regulating Mallory-Denk body formation

Fri Jun 01 00:00:00 EDT 2012

Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.

Cell transplantation

Sumitran-Holgersson S,Nowak G,Thowfeequ S,Begum S,Joshi M,Jaksch M,Kjaeldgaard A,Jorns C,Ericzon BG,Tosh D

MA1-06318 was used in immunocytochemistry to investigate the transdifferentiation of human embryonic pancreatic cells to hepatocytes

Thu Aug 13 00:00:00 EDT 2009

Antigen expression of human eccrine sweat glands.

Journal of cutaneous pathology

Li HH,Zhou G,Fu XB,Zhang L

MA1-06318 was used in immunohistochemistry to identify the phenotypic markers of human eccrine sweat glands

Sun Mar 01 00:00:00 EST 2009

Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association

Tao GZ,Strnad P,Zhou Q,Kamal A,Zhang L,Madani ND,Kugathasan S,Brant SR,Cho JH,Omary MB,Duerr RH

MA1-06318 was used in western blot to study whether variants of keratin 8 and keratin 19 are associated with inflammatory bowel disease

Sun Jul 01 00:00:00 EDT 2007

Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner.

Journal of cell science

Tao GZ,Toivola DM,Zhou Q,Strnad P,Xu B,Michie SA,Omary MB

MA1-06318 was used in western blot to study the site-specific dephosphorylation of keratin 8 by protein phosphatase-2A and how this varies according to cell type

Sat Apr 01 00:00:00 EST 2006

Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies.

Experimental cell research

Tao GZ,Nakamichi I,Ku NO,Wang J,Frolkis M,Gong X,Zhu W,Pytela R,Omary MB

MA1-06318 was used in western blot to produce and characterize human disease-related anti-keratin rabbit monoclonal antibodies

Wed Feb 15 00:00:00 EST 2006

Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.

Journal of hepato-biliary-pancreatic surgery

Yesim G,Gupse T,Zafer U,Ahmet A

MA1-06318 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver

Thu Jul 06 00:00:00 EDT 2006

Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease.

Hepatology (Baltimore, Md.)

Toivola DM,Ku NO,Resurreccion EZ,Nelson DR,Wright TL,Omary MB

MA1-06318 was used in western blot to study keratin 8 and keratin 18 hyperphosphorylation as markers for progression of human liver disease

Sun Aug 01 00:00:00 EDT 2004

Keratin 8 mutations in patients with cryptogenic liver disease.

The New England journal of medicine

Ku NO,Gish R,Wright TL,Omary MB

MA1-06318 was used in western blot to study mutations of keratin 8 in cryptogenic liver disease patients

Thu May 24 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: CK-8; Cytokeratin endo A; Cytokeratin-8; K8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8

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Gene Aliases: CARD2; CK-8; CK8; CYK8; CYKER; K2C8; K8; KO; Krt2-8; KRT8

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UniProt ID: (Human) P05787, (Rat) Q10758

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Entrez Gene ID: (Rabbit) 100348891, (Human) 3856, (Rat) 25626

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Function(s)

structural molecule activity protein binding protein complex binding scaffold protein binding

Process(es)

tumor necrosis factor-mediated signaling pathway sarcomere organization response to hydrostatic pressure response to other organism cell differentiation involved in embryonic placenta development extrinsic apoptotic signaling pathway hepatocyte apoptotic process viral process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin 8 Monoclonal Antibody (M20)

Cytokeratin 8 Antibody (MA1-06318) in ICC/IF

Product Details

MA1-06318

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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