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Invitrogen
Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody (SU0338)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 14
Cytokeratin 8 Antibody (MA5-32118) in ICC/IF
Immunofluorescence analysis of KRT8 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody (SU0338) (Product # MA5-32118) at 1:500 in 0.1% BSA, incubated at... View More
Product Details
MA5-32118
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SU0338
Immunogen
Synthetic peptide within Human Cytokeratin 8 aa 321-370
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809410
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CK-8; cytokeratin 8; Cytokeratin endo A; Cytokeratin-8; K8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8
Gene Aliases: AA960620; AL022697; AU019895; CARD2; CK-8; CK8; CYK8; EndoA; K2C8; K8; KO; Krt-2.8; Krt2-8; KRT8
UniProt ID: (Human) P05787, (Mouse) P11679
Entrez Gene ID: (Human) 3856, (Mouse) 16691
cell morphogenesis involved in differentiation
multicellular organism development
tumor necrosis factor-mediated signaling pathway
sarcomere organization
response to hydrostatic pressure
response to other organism
cell differentiation involved in embryonic placenta development
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
viral process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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