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Invitrogen
Cytokeratin Pan Monoclonal Antibody (7H8)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
Cytokeratin Pan Antibody (MA5-15507) in ICC/IF
Immunofluorescence analysis of Cytokeratin Pan was performed using 70% confluent log phase A-431 cells. The cells were fixed and permeabilized with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin Pan Monoclonal Antibody (7H8) (Product # MA5-15507, 1:100 dilution) in 0.1% BSA, incubated at 4 degree celsius overnight and the... View More
Product Details
MA5-15507
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7H8
Immunogen
Purified recombinant fragment of Cytokeratin 5 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10990047
Product Specific Information
MA5-15507 targets Cytokeratin(Pan) in indirect ELISA, IF and IHC applications and shows reactivity with Human samples.
The MA5-15507 immunogen is purified recombinant fragment of Cytokeratin 5 expressed in E. Coli.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 39.1; 40-kDa keratin intermediate filament; 58 kDa cytokeratin; 65 kDa cytokeratin; 67 kDa cytokeratin; Cell proliferation-inducing gene 46 protein; CK-1; CK-10; CK-13; CK-14; CK-15; CK-16; CK-17; CK-18; CK-19; CK-1B; CK-2e; CK-2P; CK-3; CK-4; CK-5; CK-6A; CK-6D; CK-7; CK-8; cytokeratin 1; cytokeratin 10; cytokeratin 13; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 2; cytokeratin 3; cytokeratin 4; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; cytokeratin 7; Cytokeratin-1; Cytokeratin-10; Cytokeratin-13; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-17; Cytokeratin-18; Cytokeratin-19; Cytokeratin-1B; Cytokeratin-2e; Cytokeratin-2P; Cytokeratin-3; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Cytokeratin-7; Cytokeratin-8; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; Epithelial keratin-2e; focal non-epidermolytic palmoplantar keratoderma; Hair alpha protein; HMWCK; K1; K10; K13; K14; K15; K16; K17; K18; K19; K1B; K2e; K2P; K3; K4; K5; K6A; K7; K76; K77; K8; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 1, type II; keratin 10, type I; keratin 13, type I; keratin 14, type I; keratin 15, type I; keratin 16, type I; keratin 17, type I; keratin 18, type I; keratin 19, type I; Keratin 1B; keratin 2, type II; keratin 2p; keratin 3, type II; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, , type II; keratin 6A, type II; keratin 7, type II; keratin 76, type II; keratin 77, type II; keratin 8, type II; keratin K6h; Keratin type II cytoskeletal 5; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 13; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 17; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 1b; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 2 oral; Keratin, type II cytoskeletal 3; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 8; Keratin-1; Keratin-10; Keratin-13; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-17; Keratin-18; Keratin-19; Keratin-2 epidermis; Keratin-2e; Keratin-3; Keratin-4; Keratin-5; Keratin-6A; keratin-6C; Keratin-7; Keratin-76; Keratin-77; Keratin-8; KRT1B; pan Cytokeratin; pan keratin; pankeratin; Sarcolectin; type I cytoskeletal 15; type II cytoskeletal 1b; type II mesothelial keratin K7; Type-II keratin Kb1; type-II keratin Kb12; Type-II keratin Kb2; Type-II keratin Kb3; Type-II Keratin Kb39; Type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb7; Type-II keratin Kb8; Type-II keratin Kb9
Gene Aliases: 39.1; BCIE; BIE; CARD2; CK-17; CK-18; CK-2e; CK-4; CK-6C; CK-6E; CK-8; CK1; CK10; CK13; CK14; CK15; CK16; CK19; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK18; CYK4; CYK8; DDD; DDD1; EBS2; EBS3; EBS4; EHK; EHK1; EPPK; FNEPPK; HUMCYT2A; K1; K10; K13; K14; K15; K16; K17; K18; K19; K1B; K1CO; K1CP; K1CS; K2C7; K2C8; K2e; K3; K4; K5; K6A; K6C; K6D; K7; K8; KO; KPP; KRT1; KRT10; KRT13; KRT14; KRT15; KRT16; KRT16A; KRT17; KRT18; KRT19; KRT1A; KRT1B; KRT2; KRT2A; KRT2B; KRT2E; KRT2P; KRT3; KRT4; KRT5; KRT5A; KRT6A; KRT6C; KRT6D; KRT7; KRT76; KRT77; KRT8; KRTA; KRTB; KRTE; NEPPK; NFJ; PC; PC1; PC2; PC3; PCHC1; PIG46; SCL; WSN1; WSN2
receptor activity
structural molecule activity
protein binding
carbohydrate binding
structural constituent of epidermis
structural constituent of cytoskeleton
keratin filament binding
scaffold protein binding
MHC class II receptor activity
MHC class II protein binding
poly(A) RNA binding
cadherin binding involved in cell-cell adhesion
structural constituent of muscle
protein complex binding
molecular_function
epithelial cell differentiation
intermediate filament cytoskeleton organization
cytoskeleton organization
negative regulation of epithelial cell proliferation
complement activation, lectin pathway
retina homeostasis
response to oxidative stress
fibrinolysis
regulation of angiogenesis
negative regulation of inflammatory response
establishment of skin barrier
Notch signaling pathway
viral process
response to estrogen
sarcomere organization
cell differentiation involved in embryonic placenta development
epidermis development
keratinization
keratinocyte activation
keratinocyte proliferation
keratinocyte migration
cell cycle
anatomical structure morphogenesis
tumor necrosis factor-mediated signaling pathway
Golgi to plasma membrane CFTR protein transport
negative regulation of apoptotic process
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
cell-cell adhesion
signal transduction
positive regulation of cell growth
hair follicle morphogenesis
intermediate filament organization
positive regulation of translation
positive regulation of hair follicle development
biological_process
morphogenesis of an epithelium
inflammatory response
aging
cell proliferation
keratinocyte differentiation
negative regulation of cell migration
hair cycle
innate immune response
mitophagy in response to mitochondrial depolarization
sebaceous gland development
response to radiation
tongue morphogenesis
cellular response to retinoic acid
response to zinc ion
response to ionizing radiation
hemidesmosome assembly
intermediate filament bundle assembly
response to hydrostatic pressure
response to other organism
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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