Invitrogen

Cytokeratin Pan Monoclonal Antibody (C11)

Product Details

MA1-12594

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Fish, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C11

Immunogen

Cytoskeleton preparation from human A431 carcinoma Cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1075492

Product Specific Information

This antibody detects cytokeratins 4, 5, 6, 8, 10, 13, 18.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Optimisation of immunofluorescence methods to determine MCT1 and MCT4 expression in circulating tumour cells.

BMC cancer

Kershaw S,Cummings J,Morris K,Tugwood J,Dive C

MA1-12594 was used in western blot to develop an immunofluorescence method to assess MCT1 and MCT4 in circulating tumor cells and as them as biomarkers of AZD3965 in cancer patients

Sun May 10 00:00:00 EDT 2015

Induction of persistent colitis by a human commensal, enterotoxigenic Bacteroides fragilis, in wild-type C57BL/6 mice.

Infection and immunity

Rhee KJ,Wu S,Wu X,Huso DL,Karim B,Franco AA,Rabizadeh S,Golub JE,Mathews LE,Shin J,Sartor RB,Golenbock D,Hamad AR,Gan CM,Housseau F,Sears CL

MA1-12594 was used in western blot to develop and characterize a murine model to study enterotoxigenic Bacteroides fragilis infection

Wed Apr 01 00:00:00 EDT 2009

Characterization of goldfish fin cells in culture: some evidence of an epithelial cell profile.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Mauger PE,Labbé C,Bobe J,Cauty C,Leguen I,Baffet G,Le Bail PY

MA1-12594 was used in immunocytochemistry to characterize goldfish fin cells through morphological, immunochemical and molecular methods

Sun Mar 01 00:00:00 EST 2009

Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development.

The Journal of experimental medicine

Song R,Kim YW,Koo BK,Jeong HW,Yoon MJ,Yoon KJ,Jun DJ,Im SK,Shin J,Kong MP,Kim KT,Yoon K,Kong YY

MA1-12594 was used in immunohistochemistry to study the essential role of mind bomb 1 in the development of T cells and marginal zone B cells

Mon Oct 27 00:00:00 EDT 2008

Inhibition of VEGFR2 prevents DMBA-induced mammary tumor formation.

Laboratory investigation; a journal of technical methods and pathology

Heffelfinger SC,Yan M,Gear RB,Schneider J,LaDow K,Warshawsky D

MA1-12594 was used in immunohistochemistry to investigate the effect of VEGFR2 blockade on breast cancer development stimulated by DMBA

Sun Aug 01 00:00:00 EDT 2004

Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells.

Molecular and cellular biology

Gilbert S,Loranger A,Marceau N

MA1-12594 was used in western blot to study the role of keratins in modulating cFlip and ERK1/2 apoptotic signaling in epithelial cells

Sun Aug 01 00:00:00 EDT 2004

TNP-470 inhibits 7,12-dimethylbenz[a]anthracene-induced mammary tumor formation when administered before the formation of carcinoma in situ but is not additive with tamoxifen.

Laboratory investigation; a journal of technical methods and pathology

Heffelfinger SC,Gear RB,Schneider J,LaDow K,Yan M,Lu F,Pyle AL,Warshawsky D

MA1-12594 was used in immunohistochemistry to investigate the effect of TNP-470 on breast tumor development stimulated with DMBA

Tue Jul 01 00:00:00 EDT 2003

Evidence for modulation of a 24K protein in human endometrium during the menstrual cycle.

The Journal of clinical endocrinology and metabolism

Ciocca DR,Asch RH,Adams DJ,McGuire WL

MA1-12594 was used in immunohistochemistry to investigate the expression and localization of 24K protein in the endometrium of regularly cycling women

Thu Sep 01 00:00:00 EDT 1983

Bioinformatics

Protein Aliases: 39.1; 40-kDa keratin intermediate filament; 58 kDa cytokeratin; 65 kDa cytokeratin; 67 kDa cytokeratin; Cell proliferation-inducing gene 46 protein; CK-1; CK-10; CK-13; CK-14; CK-15; CK-16; CK-17; CK-18; CK-19; CK-1B; CK-2e; CK-2P; CK-3; CK-4; CK-5; CK-6A; CK-6D; CK-7; CK-8; cytokeratin 1; cytokeratin 10; cytokeratin 13; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 2; cytokeratin 3; cytokeratin 4; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; cytokeratin 7; Cytokeratin-1; Cytokeratin-10; Cytokeratin-13; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-17; Cytokeratin-18; Cytokeratin-19; Cytokeratin-1B; Cytokeratin-2e; Cytokeratin-2P; Cytokeratin-3; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Cytokeratin-7; Cytokeratin-8; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; Epithelial keratin-2e; focal non-epidermolytic palmoplantar keratoderma; Hair alpha protein; HMWCK; K1; K10; K13; K14; K15; K16; K17; K18; K19; K1B; K2e; K2P; K3; K4; K5; K6A; K7; K76; K77; K8; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 1, type II; keratin 10, type I; keratin 13, type I; keratin 14, type I; keratin 15, type I; keratin 16, type I; keratin 17, type I; keratin 18, type I; keratin 19, type I; Keratin 1B; keratin 2, type II; keratin 2p; keratin 3, type II; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, , type II; keratin 6A, type II; keratin 7, type II; keratin 76, type II; keratin 77, type II; keratin 8, type II; keratin K6h; Keratin type II cytoskeletal 5; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 13; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 17; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 1b; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 2 oral; Keratin, type II cytoskeletal 3; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 8; Keratin-1; Keratin-10; Keratin-13; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-17; Keratin-18; Keratin-19; Keratin-2 epidermis; Keratin-2e; Keratin-3; Keratin-4; Keratin-5; Keratin-6A; keratin-6C; Keratin-7; Keratin-76; Keratin-77; Keratin-8; KRT1B; pan Cytokeratin; pan keratin; pankeratin; Sarcolectin; type I cytoskeletal 15; type II cytoskeletal 1b; type II mesothelial keratin K7; Type-II keratin Kb1; type-II keratin Kb12; Type-II keratin Kb2; Type-II keratin Kb3; Type-II Keratin Kb39; Type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb7; Type-II keratin Kb8; Type-II keratin Kb9

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Gene Aliases: 39.1; BCIE; BIE; CARD2; CK-17; CK-18; CK-2e; CK-4; CK-6C; CK-6E; CK-8; CK1; CK10; CK13; CK14; CK15; CK16; CK19; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK18; CYK4; CYK8; DDD; DDD1; EBS2; EBS3; EBS4; EHK; EHK1; EPPK; FNEPPK; HUMCYT2A; K1; K10; K13; K14; K15; K16; K17; K18; K19; K1B; K1CO; K1CP; K1CS; K2C7; K2C8; K2e; K3; K4; K5; K6A; K6C; K6D; K7; K8; KO; KPP; KRT1; KRT10; KRT13; KRT14; KRT15; KRT16; KRT16A; KRT17; KRT18; KRT19; KRT1A; KRT1B; KRT2; KRT2A; KRT2B; KRT2E; KRT2P; KRT3; KRT4; KRT5; KRT5A; KRT6A; KRT6C; KRT6D; KRT7; KRT76; KRT77; KRT8; KRTA; KRTB; KRTE; NEPPK; NFJ; PC; PC1; PC2; PC3; PCHC1; PIG46; SCL; WSN1; WSN2

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Function(s)

receptor activity structural molecule activity protein binding carbohydrate binding structural constituent of epidermis structural constituent of cytoskeleton keratin filament binding scaffold protein binding MHC class II receptor activity MHC class II protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion structural constituent of muscle protein complex binding molecular_function

Process(es)

epithelial cell differentiation intermediate filament cytoskeleton organization cytoskeleton organization negative regulation of epithelial cell proliferation complement activation, lectin pathway retina homeostasis response to oxidative stress fibrinolysis regulation of angiogenesis negative regulation of inflammatory response establishment of skin barrier Notch signaling pathway viral process response to estrogen sarcomere organization cell differentiation involved in embryonic placenta development epidermis development keratinization keratinocyte activation keratinocyte proliferation keratinocyte migration cell cycle anatomical structure morphogenesis tumor necrosis factor-mediated signaling pathway Golgi to plasma membrane CFTR protein transport negative regulation of apoptotic process extrinsic apoptotic signaling pathway hepatocyte apoptotic process cell-cell adhesion signal transduction positive regulation of cell growth hair follicle morphogenesis intermediate filament organization positive regulation of translation positive regulation of hair follicle development biological_process morphogenesis of an epithelium inflammatory response aging cell proliferation keratinocyte differentiation negative regulation of cell migration hair cycle innate immune response mitophagy in response to mitochondrial depolarization sebaceous gland development response to radiation tongue morphogenesis cellular response to retinoic acid response to zinc ion response to ionizing radiation hemidesmosome assembly intermediate filament bundle assembly response to hydrostatic pressure response to other organism

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin Pan Monoclonal Antibody (C11)

Product Details

MA1-12594

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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