Invitrogen

Cytokeratin Pan Type I Monoclonal Antibody (AE1), eBioscience™

Cytokeratin Pan Type I Antibody in Western Blot (WB)

FIGURE: 1 / 1

Cytokeratin Pan Type I Antibody (14-9001-82) in WB

MCF7 cell lysates prepared under reducing conditions were resolved by SDS-PAGE then immunoblotted with 2 µg/mL of Anti-Acidic Cytokeratin Purified.Bands were visualized using Anti-Mouse IgG HRP.

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Product Details

14-9001-82

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rhesus monkey, Rat

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834477

Product Specific Information

Description: The monoclonal antibody AE1 recognizes many members of the acidic (Type I) family of cytokeratins. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these cytokeratins, and their expression can help identify the origin of a neoplasm.

The AE1 monoclonal antibody recognizes 56.5, 54', 50, 50', 48, and 40 kDa proteins (also known as CK10, 14, 15, 16 and 19) in mouse, human, rat, primate (cynomolgus and rhesus), dog, cat, rabbit and chicken.

Applications Reported: This AE1 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This AE1 antibody has been tested by western blot on SDS-reduced lysates from MCF7 cells at less than or equal to 2 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Comparison of Multiplexed Immunofluorescence Imaging to Chromogenic Immunohistochemistry of Skin Biomarkers in Response to Monkeypox Virus Infection.

Viruses

Sood A,Sui Y,McDonough E,Santamaría-Pang A,Al-Kofahi Y,Pang Z,Jahrling PB,Kuhn JH,Ginty F

14-9001-82 was used in Immunocytochemistry to determine whether multiplexed tissue immunofluorescence is comparable to manual H-scoring of chromogenic stains.

Thu Jul 23 00:00:00 EDT 2020

Analysis of cellular autofluorescence in touch samples by flow cytometry: implications for front end separation of trace mixture evidence.

Analytical and bioanalytical chemistry

Katherine Philpott M,Stanciu CE,Kwon YJ,Bustamante EE,Greenspoon SA,Ehrhardt CJ

14-9001 was used in Flow cytometry/Cell sorting to indicate that autofluorescence levels in touch samples are influenced by a donor's contact with specific material prior to handling the collection substrate.

Sat Jul 01 00:00:00 EDT 2017

Flow cytometry dataset for cells collected from touched surfaces.

F1000Research

Kwon YJ,Stanciu CE,Philpott MK,Ehrhardt CJ

14-9001 was used in Flow cytometry/Cell sorting to create a dataset containing antibody hybridization surveys using Human Leukocyte Antigen and Cytokeratin probes.

Tue Dec 15 00:00:00 EST 2020

Bioinformatics

Protein Aliases: 40-kDa keratin intermediate filament; 56 kDa cytokeratin; Acidic Cytokeratin; alpha keratin; Cell proliferation-inducing gene 46 protein; CK-10; CK-14; CK-15; CK-16; CK-18; CK-19; CK-9; cytokeratin 10; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 9; Cytokeratin endo B; Cytokeratin-10; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-18; Cytokeratin-19; Cytokeratin-9; Endo B; epidermal keratin 10; epidermolysis bullosa simplex, Dowling-Meara, Koebner; epithelial keratin 10; Epithelial keratin-10; focal non-epidermolytic palmoplantar keratoderma; GK-19; HMWCK; intermediate filament protein; K10; K14; K15; K16; K18; K19; K9; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin 15; keratin 15, type I; keratin 16 (focal non-epidermolytic palmoplantar keratoderma); keratin 16, type I; keratin 18, type I; keratin 19; keratin 19, type I; keratin 9 (epidermolytic palmoplantar keratoderma); keratin 9, type I; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 1, acidic, gene 9; Keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 59 kDa; Keratin, type I cytoskeletal 9; keratin, type I, 40-kd; Keratin-10; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-18; Keratin-19; Keratin-9; krt10 {ECO:0000250|UniProtKB:P13645}; krt15 {ECO:0000250|UniProtKB:P19012}; krt18 {ECO:0000250|UniProtKB:P05783}; krt9; pan keratin; pankeratin; Spermatid perinuclear ring manchette protein K9; suprabasal cytokeratin 10; type I alpha-keratin 14; type I alpha-keratin 15; type I cytoskeletal 15; type I cytoskeletal 9; type I keratin KA10; Type I keratin Ka14; Type I keratin Ka15; type I keratin KA16; Type I keratin Ka19

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Gene Aliases: AI324768; AI528832; AI626930; AI663979; BCIE; BIE; CK-10; CK-14; CK-18; CK-9; CK10; CK14; CK15; CK16; CK18; CK19; CYK18; D130054E02Rik; EBS3; EBS4; EHK; EndoC; EPPK; FNEPPK; GK-19; K10; K14; K15; K16; K18; K19; K1C1; K1CO; K1CP; K1CS; K9; Ka10; Ka14; Ka15; Ka16; Ka19; KER10; keratin-19; Kerd; KPP; Krt-1.10; Krt-1.14; Krt-1.19; Krt1-10; Krt1-14; Krt1-15; Krt1-16; Krt1-18; Krt1-19; Krt1-9; KRT10; KRT14; KRT15; KRT16; KRT16A; KRT18; KRT19; KRT9; KRTB; NEPPK; NFJ; PC1; PIG46

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Function(s)

structural constituent of epidermis protein heterodimerization activity structural constituent of cytoskeleton protein binding keratin filament binding structural molecule activity scaffold protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion structural constituent of muscle protein complex binding molecular_function

Process(es)

mitophagy in response to mitochondrial depolarization epithelial cell differentiation cellular response to calcium ion morphogenesis of an epithelium inflammatory response aging biological_process keratinocyte differentiation negative regulation of cell migration keratinization hair cycle innate immune response intermediate filament cytoskeleton organization keratinocyte migration establishment of skin barrier cell cycle anatomical structure morphogenesis viral process tumor necrosis factor-mediated signaling pathway Golgi to plasma membrane CFTR protein transport negative regulation of apoptotic process extrinsic apoptotic signaling pathway hepatocyte apoptotic process cell-cell adhesion response to zinc ion response to ionizing radiation intermediate filament bundle assembly skin development intermediate filament organization spermatogenesis epidermis development hemidesmosome assembly immune system process Notch signaling pathway response to estrogen sarcomere organization cell differentiation involved in embryonic placenta development cytoskeleton organization cell proliferation intermediate filament-based process peptide cross-linking positive regulation of epidermis development protein heterotetramerization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin Pan Type I Monoclonal Antibody (AE1), eBioscience™

Cytokeratin Pan Type I Antibody (14-9001-82) in WB

Product Details

14-9001-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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