Invitrogen

Cytokeratin Pan Type I Monoclonal Antibody (AE1)

Cytokeratin Pan Type I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

Cytokeratin Pan Type I Antibody (MA1-5761) in IHC (P)

Immunohistochemistry analysis of Cytokeratin Pan Type I on colon tissue. Samples were incubated with Cytokeratin Pan Type I monoclonal antibody (Product # MA1-5761).

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Product Details

MA1-5761

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

Product Specifications

Species Reactivity

Rabbit, Rat, Bovine, Chicken, Human, Mouse, Non-human primate, Pig

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE1

Immunogen

Human epidermal keratins from P3 x 63 Ag8 cells

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1075152

Product Specific Information

MA1-5761 detects most human acidic (type I) keratins K10, K14, K15, K16, K19.

MA1-5761 has been successfully used in Western blot, immunomicroscopy and immunohistochemistry (paraffin) procedures. For Western blotting, do not use milk for blocking nonspecific binding because it contains some keratins (bovine serum albumin is recommended).

The MA1-5761 immunogen is human epidermal keratins.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Association of Merkel cell polyomavirus infection with EGFR mutation status in Chinese non-small cell lung cancer patients.

Lung cancer (Amsterdam, Netherlands)

Xu S,Jiang J,Yu X,Sheng D,Zhu T,Jin M

MA1-5761 was used in immunohistochemistry to study Chinese non-small cell lung cancer patients to deterine if there is an association between polyomavirus infection of Merkel cells and EGFR hotspot mutations

Sat Mar 01 00:00:00 EST 2014

A clinicopathological study of the significance of the proportion of choroid morphology in chordoid meningioma.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia

Lin JW,Lu CH,Lin WC,Wu YT,Huang YJ,Shih FY,Ho JT,Chuang MJ

MA1-5761 was used in immunohistochemistry to study chordoid menigioma and the significance of the proportion of chordoid morphology for the risk of recurrence

Fri Jun 01 00:00:00 EDT 2012

Clear cell meningioma with frequent chordoid features and aggressive behavior: a clinicopathologic study of ten cases at a single institution.

Journal of neuro-oncology

Chen HK,Wu YT,Lin YJ,Lin JW

MA1-5761 was used in immunohistochemistry to study the clinicopathology of clear cell meningioma with frequent chordoid features and aggressive behavior

Fri Jul 01 00:00:00 EDT 2011

Rhabdoid papillary meningioma: a clinicopathologic case series study.

Neuropathology : official journal of the Japanese Society of Neuropathology

Wu YT,Ho JT,Lin YJ,Lin JW

MA1-5761 was used in immunohistochemistry to study the clinicopathology of a series of cases of rhabdoid papillary meningioma

Thu Dec 01 00:00:00 EST 2011

The prognostic impact of O6-methylguanine DNA methyltransferase and epidermal growth factor receptor expressions on primary gliosarcoma: a clinicopathologic and immunohistochemical study of seven cases at a single institution.

Indian journal of pathology & microbiology

Lin JW,Wu YT,Chang IW

MA1-5761 was used in immunohistochemistry to study the prognostic value of MGMT and EGFR expression in primary gliosarcoma

Thu Apr 12 00:00:00 EDT 2012

Novel immunofluorescence protocol for multimarker assessment of putative disseminating breast cancer stem cells.

Applied immunohistochemistry & molecular morphology : AIMM

Balic M,Rapp N,Stanzer S,Lin H,Strutz J,Szkandera J,Daidone MG,Samonigg H,Cote RJ,Dandachi N

MA1-5761 was used in immunohistochemistry to investigate the efficiency of a new method for the assessment of putative disseminating breast cancer stem cells

Sat Jan 01 00:00:00 EST 2011

Chordoid meningioma: a clinicopathologic study of 11 cases at a single institution.

Journal of neuro-oncology

Lin JW,Ho JT,Lin YJ,Wu YT

MA1-5761 was used in immunohistochemistry to study the clinicopathology of 11 cases of chordoid meningioma

Wed Dec 01 00:00:00 EST 2010

Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.

Research in veterinary science

Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS

MA1-5761 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines

Thu Apr 01 00:00:00 EDT 2010

Melanotic Xp11 translocation renal cancer: a case with PSF-TFE3 gene fusion and up-regulation of melanogenetic transcripts.

The American journal of surgical pathology

Chang IW,Huang HY,Sung MT

MA1-5761 was used in immunohistochemistry to report on a case of melanotic Xp11 translocation renal cancer

Tue Dec 01 00:00:00 EST 2009

Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats.

Laboratory animals

Esendagli G,Yilmaz G,Canpinar H,Gunel-Ozcan A,Guc MO,Guc D

MA1-5761 was used in immunohistochemistry to investigate tumourigenesis in non-mammary tissues in the MNU-induced mammary tumour model

Thu Jan 01 00:00:00 EST 2009

An immunohistochemical study of feline endometrial adenocarcinoma.

Journal of comparative pathology

Gil da Costa RM,Santos M,Amorim I,Lopes C,Pereira PD,Faustino AM

MA1-5761 was used in immunohistochemistry - paraffin section to characterize feline endometrial adenocarcinomas immunohistochemically

Fri May 01 00:00:00 EDT 2009

Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.

International journal of colorectal disease

Yagci G,Unlu A,Kurt B,Can MF,Kaymakcioglu N,Cetiner S,Tufan T,Sen D

MA1-5761 was used in immunohistochemistry to study micrometastases and skip metastases in carcinoma of the colon and rectum

Thu Feb 01 00:00:00 EST 2007

Mediastinal intraoperative radioisotope sentinel lymph node mapping in non-small-cell lung cancer.

Nuclear medicine communications

Atinkaya C,Ozlem Küçük N,Koparal H,Aras G,Sak SD,Ozdemir N

MA1-5761 was used in immunohistochemistry to develop a method to map sentinel lymph nodes in non-small cell lung cancer patients

Mon Aug 01 00:00:00 EDT 2005

Possible association between HPV16 E7 protein level and cytokeratin 19.

International journal of cancer

Favia G,Kanduc D,Lo Muzio L,Lucchese A,Serpico R

MA1-5761 was used in western blot to study the association between HPV16 E7 protein levels and cytokeratin 19

Mon Sep 20 00:00:00 EDT 2004

Ectopic thyroid in the gallbladder: report of a case.

Surgery today

Ihtiyar E,Isiksoy S,Algin C,Sahin A,Erkasap S,Yasar B

MA1-5761 was used in immunohistochemistry to describe an unusual case of ectopic thyroid tissue

Fri Feb 27 00:00:00 EST 2004

Bioinformatics

Protein Aliases: 40-kDa keratin intermediate filament; 56 kDa cytokeratin; Acidic Cytokeratin; alpha keratin; Cell proliferation-inducing gene 46 protein; CK-10; CK-14; CK-15; CK-16; CK-18; CK-19; CK-9; cytokeratin 10; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 9; Cytokeratin endo B; Cytokeratin VIB; cytokeratin VII; Cytokeratin-10; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-18; Cytokeratin-19; Cytokeratin-6B; cytokeratin-7; Cytokeratin-9; Endo B; epidermal keratin 10; epidermal keratin VII; epidermolysis bullosa simplex, Dowling-Meara, Koebner; epidermolytic hyperkeratosis; keratosis palmaris et plantaris; focal non-epidermolytic palmoplantar keratoderma; GK-19; HMWCK; intermediate filament protein; K10; K14; K15; K16; K18; K19; K9; keratin 10 (epidermolytic hyperkeratosis); keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin 15; keratin 15, type I; keratin 16, type I; keratin 18, type I; keratin 19; keratin 19, type I; keratin 9 (epidermolytic palmoplantar keratoderma); keratin 9, type I; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 1, acidic, gene 9; Keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 59 kDa; Keratin, type I cytoskeletal 9; keratin, type I, 40-kd; Keratin-10; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-18; Keratin-19; Keratin-9; krt10 {ECO:0000250|UniProtKB:P13645}; krt15 {ECO:0000250|UniProtKB:P19012}; krt18 {ECO:0000250|UniProtKB:P05783}; krt9; pan keratin; pankeratin; Spermatid perinuclear ring manchette protein K9; suprabasal cytokeratin 10; type I alpha-keratin 14; type I alpha-keratin 15; type I cytoskeletal 15; type I cytoskeletal 9; type I keratin KA10; Type I keratin Ka14; Type I keratin Ka15; type I keratin KA16; Type I keratin Ka19; unnamed protein product

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Gene Aliases: AI324768; AI528832; AI626930; AI663979; BCIE; BIE; CK-14; CK-18; CK-9; CK10; CK14; CK15; CK16; CK18; CK19; CYK18; D130054E02Rik; EBS3; EBS4; EHK; EndoC; EPPK; FNEPPK; GK-19; K10; K14; K15; K16; K18; K19; K1C1; K1CO; K1CP; K1CS; K9; Ka10; Ka14; Ka15; Ka16; Ka19; keratin-19; Kerd; KPP; Krt-1.10; Krt-1.14; Krt-1.19; Krt1-10; Krt1-14; Krt1-15; Krt1-16; Krt1-18; Krt1-19; Krt1-9; KRT10; KRT14; KRT15; KRT16; KRT16A; KRT18; KRT19; KRT9; KRTB; NEPPK; NFJ; PC1; PIG46

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Function(s)

structural molecule activity protein heterodimerization activity structural constituent of epidermis structural constituent of cytoskeleton protein binding keratin filament binding scaffold protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion structural constituent of muscle protein complex binding molecular_function

Process(es)

mitophagy in response to mitochondrial depolarization Notch signaling pathway response to estrogen sarcomere organization cell differentiation involved in embryonic placenta development epithelial cell differentiation cellular response to calcium ion morphogenesis of an epithelium inflammatory response aging biological_process keratinocyte differentiation negative regulation of cell migration keratinization hair cycle innate immune response intermediate filament cytoskeleton organization keratinocyte migration establishment of skin barrier cell cycle anatomical structure morphogenesis viral process tumor necrosis factor-mediated signaling pathway Golgi to plasma membrane CFTR protein transport negative regulation of apoptotic process extrinsic apoptotic signaling pathway hepatocyte apoptotic process cell-cell adhesion response to zinc ion response to ionizing radiation intermediate filament bundle assembly positive regulation of epidermis development protein heterotetramerization spermatogenesis epidermis development hemidesmosome assembly skin development intermediate filament organization immune system process cytoskeleton organization cell proliferation intermediate filament-based process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin Pan Type I Monoclonal Antibody (AE1)

Cytokeratin Pan Type I Antibody (MA1-5761) in IHC (P)

Product Details

MA1-5761

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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