Proteintech

DAT Polyclonal Antibody

DAT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DAT Antibody (22524-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 22524-1-AP) DAT antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DAT Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

DAT Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22524-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DAT Fusion Protein Ag18297 (1-62 aa encoded by BC133003)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSKSKCSVG LMSSVVAPAK EPNAVGPKEV ELILVKEQNG VQLTSSTLTN PRQSPVEAQD RET (1-62 aa encoded by BC133003)

Target Information

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Crucial neuroprotective roles of the metabolite BH4 in dopaminergic neurons.

bioRxiv : the preprint server for biology

Cronin SJF,Yu W,Hale A,Licht-Mayer S,Crabtree MJ,Korecka JA,Tretiakov EO,Sealey-Cardona M,Somlyay M,Onji M,An M,Fox JD,Turnes BL,Gomez-Diaz C,da Luz Scheffer D,Cikes D,Nagy V,Weidinger A,Wolf A,Reither H,Chabloz A,Kavirayani A,Rao S,Andrews N,Latremoliere A,Costigan M,Douglas G,Freitas FC,Pifl C,Walz R,Konrat R,Mahad DJ,Koslov AV,Latini A,Isacson O,Harkany T,Hallett PJ,Bagby S,Woolf CJ,Channon KM,Je HS,Penninger JM

Mon May 08 00:00:00 EDT 2023

Components of the sympathetic nervous system as targets to modulate inflammation - rheumatoid arthritis synovial fibroblasts as neuron-like cells?

Journal of inflammation (London, England)

Cheng X,Lowin T,Honke N,Pongratz G

Tue Mar 14 00:00:00 EDT 2023

Components of the sympathetic nervous system as targets to modulate inflammation - rheumatoid arthritis synovial fibroblasts as neuron-like cells?

Journal of inflammation (London, England)

Cheng X,Lowin T,Honke N,Pongratz G

Tue Mar 14 00:00:00 EDT 2023

Exendin-4 and linagliptin attenuate neuroinflammation in a mouse model of Parkinson's disease.

Neural regeneration research

Yu HY,Sun T,Wang Z,Li H,Xu D,An J,Wen LL,Li JY,Li W,Feng J

Tue Aug 01 00:00:00 EDT 2023

Electroacupuncture alleviated depression-like behaviors in ventromedial prefrontal cortex of chronic unpredictable mild stress-induced rats: Increasing synaptic transmission and phosphorylating dopamine transporter.

CNS neuroscience & therapeutics

Cai X,Wu M,Zhang Z,Liu H,Huang S,Song J,Ren S,Huang Y

Fri Sep 01 00:00:00 EDT 2023

Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats.

Journal of affective disorders

Chen B,Xu J,Chen S,Mou T,Wang Y,Wang H,Zhang Z,Ren F,Wang Z,Jin K,Lu J

Wed Mar 15 00:00:00 EDT 2023

Sleep Disturbance Alters Cocaine-Induced Locomotor Activity: Involvement of Striatal Neuroimmune and Dopamine Signaling.

Biomedicines

Roodsari SK,Cheng Y,Reed KM,Wellman LL,Sanford LD,Kim WK,Guo ML

Wed May 18 00:00:00 EDT 2022

Focused Ultrasound Promotes the Delivery of Gastrodin and Enhances the Protective Effect on Dopaminergic Neurons in a Mouse Model of Parkinson's Disease.

Frontiers in cellular neuroscience

Wang Y,Luo K,Li J,Liao Y,Liao C,Chen WS,Chen M,Ao L

Sat Jul 16 00:00:00 EDT 2022

FASEB bioAdvances

Sun X,Kato H,Sato H,Han X,Hirofuji Y,Kato TA,Sakai Y,Ohga S,Fukumoto S,Masuda K

Fri Jul 01 00:00:00 EDT 2022

FMR1 deletion in rats induces hyperactivity with no changes in striatal dopamine transporter availability.

Scientific reports

D'Elia A,Schiavi S,Manduca A,Rava A,Buzzelli V,Ascone F,Orsini T,Putti S,Soluri A,Galli F,Soluri A,Mattei M,Cicconi R,Massari R,Trezza V

Thu Dec 29 00:00:00 EST 2022

FMR1 deletion in rats induces hyperactivity with no changes in striatal dopamine transporter availability.

Scientific reports

D'Elia A,Schiavi S,Manduca A,Rava A,Buzzelli V,Ascone F,Orsini T,Putti S,Soluri A,Galli F,Soluri A,Mattei M,Cicconi R,Massari R,Trezza V

Thu Dec 29 00:00:00 EST 2022

FMR1 deletion in rats induces hyperactivity with no changes in striatal dopamine transporter availability.

Scientific reports

D'Elia A,Schiavi S,Manduca A,Rava A,Buzzelli V,Ascone F,Orsini T,Putti S,Soluri A,Galli F,Soluri A,Mattei M,Cicconi R,Massari R,Trezza V

Thu Dec 29 00:00:00 EST 2022

Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration.

Movement disorders : official journal of the Movement Disorder Society

Yuan X,Yang Y,Liu C,Tian Y,Xia D,Liu Z,Pan L,Xiong M,Xiong J,Meng L,Zhang Z,Ye K,Jiang H,Zhang Z

Thu Sep 01 00:00:00 EDT 2022

The neuroprotective effects of isoquercitrin purified from apple pomace by high-speed countercurrent chromatography in the MPTP acute mouse model of Parkinson's disease.

Food & function

Liu C,Wang W,Li H,Liu J,Zhang P,Cheng Y,Qin X,Hu Y,Wei Y

Wed Jul 07 00:00:00 EDT 2021

Exposure to Melamine cyanuric acid in adolescent mice caused emotional disorder and behavioral disorder.

Ecotoxicology and environmental safety

Wang S,Zhang J,Zhang S,Shi F,Feng D,Feng X

Mon Mar 15 00:00:00 EDT 2021

Iron deficiency in pregnancy: Influence on sleep, behavior, and molecular markers of adult male offspring.

Journal of neuroscience research

Franco B,Mota DS,Daubian-Nosé P,Rodrigues NA,Simino LAP,de Fante T,Bezerra RMN,Manchado Gobatto FB,Manconi M,Torsoni AS,Esteves AM

Wed Dec 01 00:00:00 EST 2021

Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.

Neurobiology of disease

Zou L,Zhang X,Xiong M,Meng L,Tian Y,Pan L,Yuan X,Chen G,Wang Z,Bu L,Yao Z,Zhang Z,Ye K,Zhang Z

Thu Jul 01 00:00:00 EDT 2021

Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.

Brain pathology (Zurich, Switzerland)

Wang H,Yang H,Shi Y,Xiao Y,Yin Y,Jiang B,Ren H,Chen W,Xue Q,Xu X

Wed Sep 01 00:00:00 EDT 2021

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Nature

Qian H,Kang X,Hu J,Zhang D,Liang Z,Meng F,Zhang X,Xue Y,Maimon R,Dowdy SF,Devaraj NK,Zhou Z,Mobley WC,Cleveland DW,Fu XD

Mon Jun 01 00:00:00 EDT 2020

Gait Deficits and Loss of Striatal Tyrosine Hydroxlase/Trk-B are Restored Following 7,8-Dihydroxyflavone Treatment in a Progressive MPTP Mouse Model of Parkinson's Disease.

Neuroscience

Massaquoi MS,Liguore WA,Churchill MJ,Moore C,Melrose HL,Meshul CK

Fri May 01 00:00:00 EDT 2020

PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.

Journal of lipid research

Kuge H,Miyamoto I,Yagyu KI,Honke K

Tue Dec 01 00:00:00 EST 2020

PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.

Journal of lipid research

Kuge H,Miyamoto I,Yagyu KI,Honke K

Tue Dec 01 00:00:00 EST 2020

Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.

Molecular medicine (Cambridge, Mass.)

Li S,Zhang H,Wang A,Liu Y,Liu H,Yue F,Abulaiti X,Zhang C,Li L

Fri Mar 29 00:00:00 EDT 2019

The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling.

Aging

Zhu Z,Yichen W,Ziheng Z,Dinghao G,Ming L,Wei L,Enfang S,Gang H,Honda H,Jian Y

Sat Dec 28 00:00:00 EST 2019

Andrographolide alleviates Parkinsonism in MPTP-PD mice via targeting mitochondrial fission mediated by dynamin-related protein 1.

British journal of pharmacology

Geng J,Liu W,Gao J,Jiang C,Fan T,Sun Y,Qin ZH,Xu Q,Guo W,Gao J

Sun Dec 01 00:00:00 EST 2019

Glatiramer Acetate Reverses Motor Dysfunction and the Decrease in Tyrosine Hydroxylase Levels in a Mouse Model of Parkinson's Disease.

Neuroscience

Churchill MJ,Cantu MA,Kasanga EA,Moore C,Salvatore MF,Meshul CK

Wed Aug 21 00:00:00 EDT 2019

Effects of sleep disruption on stress, nigrostriatal markers, and behavior in a chronic/progressive MPTP male mouse model of parkinsonism.

Journal of neuroscience research

Xu M,Bohlen JK,Moore C,Nipper MA,Finn DA,Jones CE,Lim MM,Meshul CK

Sun Dec 01 00:00:00 EST 2019

Catalpol Exerts a Neuroprotective Effect in the MPTP Mouse Model of Parkinson's Disease.

Frontiers in aging neuroscience

Wang LY,Yu X,Li XX,Zhao YN,Wang CY,Wang ZY,He ZY

Thu Oct 01 00:00:00 EDT 2020

Downregulation of DEC1 contributes to the neurotoxicity induced by MPP⁺ by suppressing PI3K/Akt/GSK3β pathway.

CNS neuroscience & therapeutics

Zhu Z,Wang YW,Ge DH,Lu M,Liu W,Xiong J,Hu G,Li XP,Yang J

Fri Sep 01 00:00:00 EDT 2017

Downregulation of DEC1 contributes to the neurotoxicity induced by MPP⁺ by suppressing PI3K/Akt/GSK3β pathway.

CNS neuroscience & therapeutics

Zhu Z,Wang YW,Ge DH,Lu M,Liu W,Xiong J,Hu G,Li XP,Yang J

Fri Sep 01 00:00:00 EDT 2017

Echinacoside's nigrostriatal dopaminergic protection against 6-OHDA-Induced endoplasmic reticulum stress through reducing the accumulation of Seipin.

Journal of cellular and molecular medicine

Zhang Y,Long H,Zhou F,Zhu W,Ruan J,Zhao Y,Lu Y

Fri Dec 01 00:00:00 EST 2017

Echinacoside's nigrostriatal dopaminergic protection against 6-OHDA-Induced endoplasmic reticulum stress through reducing the accumulation of Seipin.

Journal of cellular and molecular medicine

Zhang Y,Long H,Zhou F,Zhu W,Ruan J,Zhao Y,Lu Y

Fri Dec 01 00:00:00 EST 2017

Exercise in an animal model of Parkinson's disease: Motor recovery but not restoration of the nigrostriatal pathway.

Neuroscience

Churchill MJ,Pflibsen L,Sconce MD,Moore C,Kim K,Meshul CK

Sun Sep 17 00:00:00 EDT 2017

Exercise in an animal model of Parkinson's disease: Motor recovery but not restoration of the nigrostriatal pathway.

Neuroscience

Churchill MJ,Pflibsen L,Sconce MD,Moore C,Kim K,Meshul CK

Sun Sep 17 00:00:00 EDT 2017

Exercise intervention increases spontaneous locomotion but fails to attenuate dopaminergic system loss in a progressive MPTP model in aged mice.

Brain research

Hood RL,Liguore WA,Moore C,Pflibsen L,Meshul CK

Thu Sep 01 00:00:00 EDT 2016

Exercise intervention increases spontaneous locomotion but fails to attenuate dopaminergic system loss in a progressive MPTP model in aged mice.

Brain research

Hood RL,Liguore WA,Moore C,Pflibsen L,Meshul CK

Thu Sep 01 00:00:00 EDT 2016

Intervention with exercise restores motor deficits but not nigrostriatal loss in a progressive MPTP mouse model of Parkinson's disease.

Neuroscience

Sconce MD,Churchill MJ,Greene RE,Meshul CK

Thu Jul 23 00:00:00 EDT 2015

Intervention with exercise restores motor deficits but not nigrostriatal loss in a progressive MPTP mouse model of Parkinson's disease.

Neuroscience

Sconce MD,Churchill MJ,Greene RE,Meshul CK

Thu Jul 23 00:00:00 EDT 2015

Protective effects of cholecystokinin-8 on methamphetamine-induced behavioral changes and dopaminergic neurodegeneration in mice.

Behavioural brain research

Gou H,Wen D,Ma C,Li M,Li Y,Zhang W,Liu L,Cong B

Wed Apr 15 00:00:00 EDT 2015

Protective effects of cholecystokinin-8 on methamphetamine-induced behavioral changes and dopaminergic neurodegeneration in mice.

Behavioural brain research

Gou H,Wen D,Ma C,Li M,Li Y,Zhang W,Liu L,Cong B

Wed Apr 15 00:00:00 EDT 2015

Protective effects of cholecystokinin-8 on methamphetamine-induced behavioral changes and dopaminergic neurodegeneration in mice.

Behavioural brain research

Gou H,Wen D,Ma C,Li M,Li Y,Zhang W,Liu L,Cong B

Wed Apr 15 00:00:00 EDT 2015

Protective effects of cholecystokinin-8 on methamphetamine-induced behavioral changes and dopaminergic neurodegeneration in mice.

Behavioural brain research

Gou H,Wen D,Ma C,Li M,Li Y,Zhang W,Liu L,Cong B

Wed Apr 15 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: DA transporter; DAT; dopamine transporter 1; Sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter), member 3; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; Solute carrier family 6 member 3; solute carrier family 6, member 3

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Gene Aliases: DAT; DAT1; PKDYS; SLC6A3

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UniProt ID: (Human) Q01959, (Rat) P23977, (Mouse) Q61327

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Entrez Gene ID: (Human) 6531, (Rat) 24898, (Mouse) 13162

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Function(s)

protease binding receptor binding dopamine transmembrane transporter activity dopamine:sodium symporter activity protein binding drug binding monoamine transmembrane transporter activity protein complex binding dopamine binding metal ion binding protein N-terminus binding protein phosphatase 2A binding neurotransmitter:sodium symporter activity symporter activity primary active transporter

Process(es)

aging lactation sensory perception of smell locomotory behavior response to iron ion monoamine transport dopamine transport adenohypophysis development response to nicotine positive regulation of multicellular organism growth regulation of dopamine metabolic process neurotransmitter biosynthetic process response to cocaine dopamine biosynthetic process dopamine catabolic process response to drug response to ethanol dopamine uptake involved in synaptic transmission response to cAMP prepulse inhibition ammonium transmembrane transport dopamine uptake neurotransmitter transport response to organic cyclic compound cation transmembrane transport transport organic cation transport sodium ion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DAT Polyclonal Antibody

DAT Antibody (22524-1-AP) in IHC (P)

Product Details

22524-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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