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NeoBiotechnologies
DCP2 (Decapping mRNA 2) Monoclonal Antibody (PCRP-DCP2-1D6)
Product Details
167227-MSM1-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
PCRP-DCP2-1D6
Immunogen
Recombinant full-length human DCP2protein
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Antibody is stable for 24 months.
Positive Control: MCF7, Daudi or Jurkatcells. Cellular Localization: Cytoplasm > P. body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.
Specificity Comments: The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay
Predicted to react with Mouse and Rat
Target Information
The removal, or decapping, of eukaryotic mRNA is an important step in the degradation of mRNA. Decapping protein 2 (DCP2) is the major mRNA decapping enzyme in cells. It is a member of the Nudix hydrolases superfamily of proteins that predominantly catalyze the hydrolysis of small nucleoside diphosphate substrates linked to another moiety. DCP2 is widely expressed in multiple tissues at varying levels, with highest expression seen in testis and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DCP2 decapping enzyme homolog; Decapping protein 2; DFLJ33245; hDpc; m7GpppN-mRNA hydrolase; mRNA decapping enzyme 2; mRNA-decapping enzyme 2; Nucleoside diphosphate-linked moiety X motif 20; nudix (nucleoside diphosphate linked moiety X)-type motif 20; nudix (nucleoside diphosphate linked moiety X)-type motif 20 (NUDT20); Nudix motif 20; nudix-type motif 20
Gene Aliases: DCP2; NUDT20
UniProt ID: (Human) Q8IU60
Entrez Gene ID: (Human) 167227
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
deadenylation-dependent decapping of nuclear-transcribed mRNA
mRNA catabolic process
regulation of mRNA stability
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
histone mRNA catabolic process
RNA phosphodiester bond hydrolysis, exonucleolytic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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