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DCT Antibody (PA5-69368) in ICC/IF
Immunofluorescence analysis of L-dopachrome tautomerase was performed using 70% confluent log phase B16-F10 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with DCT Polyclonal Antibody (Product # PA5-69368) at 1:50 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Prod... View More
Product Details
PA5-69368
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide corresponding to a region of mouse DCT
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2688598
Product Specific Information
This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
Target Information
The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteinerich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exist as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DCT; DOPAchrome conversion factor; DOPAchrome isomerase; DOPAchrome oxidoreductase; L-dopachrome Delta-isomerase; L-dopachrome tautomerase; melanogenic enzyme; tyrosinase-related protein 2; trp2; SLATY locus protein; TRP-2; Tyrosinase-related protein 2; tyrosinase-related protein-2
Gene Aliases: Dct; DT; slaty; slt; TRP-2; TRP2; Tyrp-2; Tyrp2; zgc:109860
UniProt ID: (Mouse) P29812
Entrez Gene ID: (Mouse) 13190, (Zebrafish) 58074
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