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DCX Antibody (CF501778) in ICC/IF
Immunofluorescent staining of 293T cells transiently transfected by pCMV6-ENTRY DCX(RC221891) using anti-DCX mouse monoclonal antibody (TA501778, green, upper left; merged, upper right). Cell nuclei were stained with DAPI (blue, lower left). 293T cells transfected with empty vector plasmid served as negative control (merged, lower right). (1:100)
Product Details
CF501778
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI4A3
Immunogen
Full length human recombinant protein of human DCX produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: doublecortex; Doublin; Lis-X; LIS-XD; Lissencephalin-X; Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316
Gene Aliases: DBCN; DC; DCX; LISX; SCLH; XLIS
UniProt ID: (Human) O43602
Entrez Gene ID: (Human) 1641
neuron migration
nervous system development
central nervous system development
peptidyl-serine phosphorylation
hippocampus development
layer formation in cerebral cortex
pyramidal neuron development
central nervous system projection neuron axonogenesis
intracellular signal transduction
protein autophosphorylation
axon extension
dendrite morphogenesis
response to electrical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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