Proteintech

DDA1 Polyclonal Antibody

DDA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

DDA1 Antibody (14995-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 14995-1-AP (DDA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DDA1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14995-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DDA1 Fusion Protein Ag6981 (1-102 aa encoded by BC000615)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MADFLKGLP VYNKSNFSRF HADSVCKASN RRPSVYLPTR EYPSEQIIVT EKTNILLRYL HQQWDKKNAA KKRDQEQVEL EGESSAPPRK VARTDSPDMH EDT (1-102 aa encoded by BC000615)

Target Information

Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:17452440, PubMed:28437394, PubMed:28302793, PubMed:31686031, PubMed:31819272). In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex (PubMed:31686031, PubMed:31819272). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.

EMBO molecular medicine

Zhu L,Retana D,García-Gómez P,Álvaro-Espinosa L,Priego N,Masmudi-Martín M,Yebra N,Miarka L,Hernández-Encinas E,Blanco-Aparicio C,Martínez S,Sobrino C,Ajenjo N,Artiga MJ,Ortega-Paino E,Torres-Ruiz R,Rodríguez-Perales S,Soffietti R,Bertero L,Cassoni P,Weiss T,Muñoz J,Sepúlveda JM,González-León P,Jiménez-Roldán L,Moreno LM,Esteban O,Pérez-Núñez Á,Hernández-Laín A,Toldos O,Ruano Y,Alcázar L,Blasco G,Fernández-Alén J,Caleiras E,Lafarga M,Megías D,Graña-Castro O,Nör C,Taylor MD,Young LS,Varešlija D,Cosgrove N,Couch FJ,Cussó L,Desco M,Mouron S,Quintela-Fandino M,Weller M,Pastor J,Valiente M

Mon Mar 07 00:00:00 EST 2022

A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.

EMBO molecular medicine

Mon Mar 07 00:00:00 EST 2022

CRL4^AMBRA1 is a master regulator of D-type cyclins.

Nature

Simoneschi D,Rona G,Zhou N,Jeong YT,Jiang S,Milletti G,Arbini AA,O'Sullivan A,Wang AA,Nithikasem S,Keegan S,Siu Y,Cianfanelli V,Maiani E,Nazio F,Cecconi F,Boccalatte F,Fenyö D,Jones DR,Busino L,Pagano M

Thu Apr 01 00:00:00 EDT 2021

Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.

Nature chemical biology

Bussiere DE,Xie L,Srinivas H,Shu W,Burke A,Be C,Zhao J,Godbole A,King D,Karki RG,Hornak V,Xu F,Cobb J,Carte N,Frank AO,Frommlet A,Graff P,Knapp M,Fazal A,Okram B,Jiang S,Michellys PY,Beckwith R,Voshol H,Wiesmann C,Solomon JM,Paulk J

Wed Jan 01 00:00:00 EST 2020

Cryptochromes-Mediated Inhibition of the CRL4^Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.

Current biology : CB

Rizzini L,Levine DC,Perelis M,Bass J,Peek CB,Pagano M

Mon Jun 17 00:00:00 EDT 2019

Cryptochromes-Mediated Inhibition of the CRL4^Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.

Current biology : CB

Rizzini L,Levine DC,Perelis M,Bass J,Peek CB,Pagano M

Mon Jun 17 00:00:00 EDT 2019

Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.

Cell reports

Ting TC,Goralski M,Klein K,Wang B,Kim J,Xie Y,Nijhawan D

Tue Nov 05 00:00:00 EST 2019

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Science (New York, N.Y.)

Han T,Goralski M,Gaskill N,Capota E,Kim J,Ting TC,Xie Y,Williams NS,Nijhawan D

Fri Apr 28 00:00:00 EDT 2017

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Science (New York, N.Y.)

Han T,Goralski M,Gaskill N,Capota E,Kim J,Ting TC,Xie Y,Williams NS,Nijhawan D

Fri Apr 28 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: cross-immune reaction antigen PCIA1; DET1- and DDB1-associated protein 1; PCIA-1; Placenta cross-immune reaction antigen 1

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Gene Aliases: 1500034J01Rik; 1700095J19Rik; C19orf58; DDA1; PCIA1; R74921

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UniProt ID: (Human) Q9BW61, (Mouse) Q9D9Z5

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Entrez Gene ID: (Human) 79016, (Mouse) 66498, (Rat) 688813

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Function(s)

protein binding molecular_function

Process(es)

biological_process regulation of proteasomal ubiquitin-dependent protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DDA1 Polyclonal Antibody

DDA1 Antibody (14995-1-AP) in ICC/IF

Product Details

14995-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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