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DDIT4 Antibody (PA5-96924) in WB
Western blot analysis of DDIT4 in extracts of various cell lines. Samples were incubated with DDIT4 Polyclonal antibody (Product # PA5-96924) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-96924
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_0619311)
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808726
Product Specific Information
Immunogen sequence: MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC; Positive Samples: A-549, K-562, DU145, MCF-7; Cellular Location: Cytoplasm, Mitochondrion, cytosol
Target Information
DDIT4 gene ontology annotations related to this gene include cellular response to dexamethasone stimulus; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; negative regulation of glycolytic process; negative regulation of TOR signaling; protein-containing complex disassembly; reactive oxygen species metabolic process; response to hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA damage-inducible transcript 4 protein; DNA-damage-inducible transcript 4; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1; RP11-442H21.1
Gene Aliases: DDIT4; Dig2; REDD-1; REDD1; RTP801
UniProt ID: (Human) Q9NX09
Entrez Gene ID: (Human) 54541
response to hypoxia
neuron migration
brain development
cell proliferation
negative regulation of peptidyl-threonine phosphorylation
neuron differentiation
negative regulation of TOR signaling
negative regulation of peptidyl-serine phosphorylation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
protein complex disassembly
negative regulation of glycolytic process
neurotrophin TRK receptor signaling pathway
defense response to virus
reactive oxygen species metabolic process
positive regulation of neuron death
negative regulation of intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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