Proteintech

DDX1 Polyclonal Antibody

DDX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DDX1 Antibody (11357-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using (Product # 11357-1-AP) (DDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DDX1 Antibody in Immunoprecipitation (IP)

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Product Details

11357-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DDX1 Fusion Protein Ag1887 (391-740 aa encoded by BC012132)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: IPQVTSDGK RLQVIVCSAT LHSFDVKKLS EKIMHFPTWV DLKGEDSVPD TVHHVVVPVN PKTDRLWERL GKSHIRTDDV HAKDNTRPGA NSPEMWSEAI KILKGEYAVR AIKEHKMDQA IIFCRTKIDC DNLEQYFIQQ GGGPDKKGHQ FSCVCLHGDR KPHERKQNLE RFKKGDVRFL ICTDVAARGI DIHGVPYVIN VTLPDEKQNY VHRIGRVGRA ERMGLAISLV ATEKEKVWYH VCSSRGKGCY NTRLKEDGGC TIWYNEMQLL SEIEEHLNCT ISQVEPDIKV PVDEFDGKVT YGQKRAAGGG SYKGHVDILA PTVQELAALE KEAQTSFLHL GYLPNQLFRT F (391-740 aa encoded by BC012132)

Target Information

DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HNRNPU facilitates antibody class-switch recombination through C-NHEJ promotion and R-loop suppression.

Cell reports

Refaat AM,Nakata M,Husain A,Kosako H,Honjo T,Begum NA

Tue Mar 28 00:00:00 EDT 2023

Knockdown of ZBTB11 impedes R-loop elimination and increases the sensitivity to cisplatin by inhibiting DDX1 transcription in bladder cancer.

Cell proliferation

Chen L,Liu Z,Tang H,Zhou Z,Chen J,Ma Z,Deng M,Li X,Wu Y,Zheng L,Zhou L,Zheng X,Liu Z

Thu Dec 01 00:00:00 EST 2022

Knockdown of ZBTB11 impedes R-loop elimination and increases the sensitivity to cisplatin by inhibiting DDX1 transcription in bladder cancer.

Cell proliferation

Chen L,Liu Z,Tang H,Zhou Z,Chen J,Ma Z,Deng M,Li X,Wu Y,Zheng L,Zhou L,Zheng X,Liu Z

Thu Dec 01 00:00:00 EST 2022

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Developmental cell

Ma Y,Liu S,Gao J,Chen C,Zhang X,Yuan H,Chen Z,Yin X,Sun C,Mao Y,Zhou F,Shao Y,Liu Q,Xu J,Cheng L,Yu D,Li P,Yi P,He J,Geng G,Guo Q,Si Y,Zhao H,Li H,Banes GL,Liu H,Nakamura Y,Kurita R,Huang Y,Wang X,Wang F,Fang G,Engel JD,Shi L,Zhang YE,Yu J

Mon Feb 22 00:00:00 EST 2021

RNA helicase, DDX3X, is actively recruited to sites of DNA damage in live cells.

DNA repair

Cargill MJ,Morales A,Ravishankar S,Warren EH

Thu Jul 01 00:00:00 EDT 2021

BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain.

Cell systems

Pourhaghighi R,Ash PEA,Phanse S,Goebels F,Hu LZM,Chen S,Zhang Y,Wierbowski SD,Boudeau S,Moutaoufik MT,Malty RH,Malolepsza E,Tsafou K,Nathan A,Cromar G,Guo H,Abdullatif AA,Apicco DJ,Becker LA,Gitler AD,Pulst SM,Youssef A,Hekman R,Havugimana PC,White CA,Blum BC,Ratti A,Bryant CD,Parkinson J,Lage K,Babu M,Yu H,Bader GD,Wolozin B,Emili A

Wed Apr 22 00:00:00 EDT 2020

Modulation of RNA Condensation by the DEAD-Box Protein eIF4A.

Cell

Tauber D,Tauber G,Khong A,Van Treeck B,Pelletier J,Parker R

Thu Feb 06 00:00:00 EST 2020

Nucleocytoplasmic shuttling of Gle1 impacts DDX1 at transcription termination sites.

Molecular biology of the cell

Sharma M,Wente SR

Thu Oct 01 00:00:00 EDT 2020

Nucleocytoplasmic shuttling of Gle1 impacts DDX1 at transcription termination sites.

Molecular biology of the cell

Sharma M,Wente SR

Thu Oct 01 00:00:00 EDT 2020

CircLONP2 enhances colorectal carcinoma invasion and metastasis through modulating the maturation and exosomal dissemination of microRNA-17.

Molecular cancer

Han K,Wang FW,Cao CH,Ling H,Chen JW,Chen RX,Feng ZH,Luo J,Jin XH,Duan JL,Li SM,Ma NF,Yun JP,Guan XY,Pan ZZ,Lan P,Xu RH,Xie D

Wed Mar 18 00:00:00 EDT 2020

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Molecular cell

Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ

Thu May 17 00:00:00 EDT 2018

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Molecular cell

Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ

Thu May 17 00:00:00 EDT 2018

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Molecular cell

Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ

Thu May 17 00:00:00 EDT 2018

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Molecular cell

Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ

Thu May 17 00:00:00 EDT 2018

RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.

Nucleic acids research

Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

Fri Dec 14 00:00:00 EST 2018

RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.

Nucleic acids research

Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

Fri Dec 14 00:00:00 EST 2018

RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.

Nucleic acids research

Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

Fri Dec 14 00:00:00 EST 2018

RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.

Nucleic acids research

Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

Fri Dec 14 00:00:00 EST 2018

DEAD box protein DDX1 promotes colorectal tumorigenesis through transcriptional activation of the LGR5 gene.

Cancer science

Tanaka K,Ikeda N,Miyashita K,Nuriya H,Hara T

Wed Aug 01 00:00:00 EDT 2018

DEAD box protein DDX1 promotes colorectal tumorigenesis through transcriptional activation of the LGR5 gene.

Cancer science

Tanaka K,Ikeda N,Miyashita K,Nuriya H,Hara T

Wed Aug 01 00:00:00 EDT 2018

A Survey of DDX21 Activity During Rev/RRE Complex Formation.

Journal of molecular biology

Hammond JA,Zhou L,Lamichhane R,Chu HY,Millar DP,Gerace L,Williamson JR

Fri Feb 16 00:00:00 EST 2018

A Survey of DDX21 Activity During Rev/RRE Complex Formation.

Journal of molecular biology

Hammond JA,Zhou L,Lamichhane R,Chu HY,Millar DP,Gerace L,Williamson JR

Fri Feb 16 00:00:00 EST 2018

DDX1 regulates alternative splicing and insulin secretion in pancreatic β cells.

Biochemical and biophysical research communications

Zhong W,Li Z,Zhou M,Xu T,Wang Y

Thu Jun 07 00:00:00 EDT 2018

DDX1 regulates alternative splicing and insulin secretion in pancreatic β cells.

Biochemical and biophysical research communications

Zhong W,Li Z,Zhou M,Xu T,Wang Y

Thu Jun 07 00:00:00 EDT 2018

BEX1 is an RNA-dependent mediator of cardiomyopathy.

Nature communications

Accornero F,Schips TG,Petrosino JM,Gu SQ,Kanisicak O,van Berlo JH,Molkentin JD

Thu Nov 30 00:00:00 EST 2017

BEX1 is an RNA-dependent mediator of cardiomyopathy.

Nature communications

Accornero F,Schips TG,Petrosino JM,Gu SQ,Kanisicak O,van Berlo JH,Molkentin JD

Thu Nov 30 00:00:00 EST 2017

RNase L activates the NLRP3 inflammasome during viral infections.

Cell host & microbe

Chakrabarti A,Banerjee S,Franchi L,Loo YM,Gale M,Núñez G,Silverman RH

Wed Apr 08 00:00:00 EDT 2015

RNase L activates the NLRP3 inflammasome during viral infections.

Cell host & microbe

Chakrabarti A,Banerjee S,Franchi L,Loo YM,Gale M,Núñez G,Silverman RH

Wed Apr 08 00:00:00 EDT 2015

DDX1 is an RNA-dependent ATPase involved in HIV-1 Rev function and virus replication.

Journal of molecular biology

Edgcomb SP,Carmel AB,Naji S,Ambrus-Aikelin G,Reyes JR,Saphire AC,Gerace L,Williamson JR

Fri Jan 06 00:00:00 EST 2012

DDX1 is an RNA-dependent ATPase involved in HIV-1 Rev function and virus replication.

Journal of molecular biology

Edgcomb SP,Carmel AB,Naji S,Ambrus-Aikelin G,Reyes JR,Saphire AC,Gerace L,Williamson JR

Fri Jan 06 00:00:00 EST 2012

Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1

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Gene Aliases: AA409185; DBP-RB; DDX1; UKVH5d

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UniProt ID: (Human) Q92499, (Rat) Q641Y8, (Mouse) Q91VR5

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Entrez Gene ID: (Human) 1653, (Rat) 84474, (Mouse) 104721

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Function(s)

DNA binding chromatin binding transcription cofactor activity RNA helicase activity double-stranded RNA binding ATP-dependent RNA helicase activity nuclease activity exonuclease activity protein binding ATP binding ATP-dependent helicase activity poly(A) binding DNA/RNA helicase activity poly(A) RNA binding nucleotide binding nucleic acid binding RNA binding helicase activity hydrolase activity

Process(es)

spliceosomal complex assembly double-strand break repair transcription, DNA-templated regulation of transcription, DNA-templated tRNA splicing, via endonucleolytic cleavage and ligation regulation of translational initiation multicellular organism development response to virus RNA secondary structure unwinding DNA duplex unwinding response to exogenous dsRNA nucleic acid phosphodiester bond hydrolysis protein localization to cytoplasmic stress granule mRNA processing tRNA processing regulation of nucleic acid-templated transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DDX1 Polyclonal Antibody

DDX1 Antibody (11357-1-AP) in IHC (P)

Product Details

11357-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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