Proteintech

DDX58 Polyclonal Antibody

DDX58 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

DDX58 Antibody (20566-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using DDX58 antibody 20566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DDX58 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20566-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 30 publications 30 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

RNA Immunoprecipitation (RIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.51 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to DDX58.

Target Information

Retinoic acid-inducible gene I, RIG-I is a pattern recognition receptor (PRR) involved in the recognition of viral dsRNA. Along with MDA5, RIG-I detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases and they perform overlapping as well as distinct roles. RIG-I is activated by dsRNAs without a 5'-triphosphate end and short dsRNAs, whereas MDA5 is activated by long dsRNAs. Once activated, both proteins signal through IPS-1 activating transcription factors NF-kappaB and IRF-3 (1) and ultimately activating apoptosis, cytokine signaling, and inflammation. RIG-I is essential for signaling by influenza A, influenza B, human respiratory syncytial virus (3), paromyxoviruses, Japanese encephalitis virus, and West Nile virus. MicroRNA-146a has been implicated in feedback inhibition of RIG-I-dependant antiviral response by negatively regulating RIG-I targets TRAF6, IRAK1, and IRAK2. Recent evidence has implicated RIG-I in the detection of cytosolic DNA through RNA polymerase III activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRIM28 negatively regulates the RLR signaling pathway by targeting MAVS for degradation via K48-linked polyubiquitination.

The Journal of biological chemistry

Chen YY,Ran XH,Ni RZ,Mu D

Mon May 01 00:00:00 EDT 2023

Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses.

Nature communications

Deng S,He W,Gong AY,Li M,Wang Y,Xia Z,Zhang XT,Huang Pacheco AS,Naqib A,Jenkins M,Swanson PC,Drescher KM,Strauss-Soukup JK,Belshan M,Chen XM

Thu Mar 16 00:00:00 EDT 2023

Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses.

Nature communications

Deng S,He W,Gong AY,Li M,Wang Y,Xia Z,Zhang XT,Huang Pacheco AS,Naqib A,Jenkins M,Swanson PC,Drescher KM,Strauss-Soukup JK,Belshan M,Chen XM

Thu Mar 16 00:00:00 EDT 2023

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion.

Signal transduction and targeted therapy

Zheng W,Zhou Z,Rui Y,Ye R,Xia F,Guo F,Liu X,Su J,Lou M,Yu XF

Fri Feb 24 00:00:00 EST 2023

The differential expression of toll like receptors and RIG-1 correlates to the severity of infectious diseases.

Annals of diagnostic pathology

Rice M,Tili E,Loghmani H,Nuovo GJ

Sat Apr 01 00:00:00 EDT 2023

Heterogeneous Nuclear Protein U Degraded the m⁶A Methylated TRAF3 Transcript by YTHDF2 To Promote Porcine Epidemic Diarrhea Virus Replication.

Journal of virology

Zhou H,Yan Y,Gao J,Ma M,Liu Y,Shi X,Zhang Q,Xu X

Tue Feb 28 00:00:00 EST 2023

Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis.

Journal of innate immunity

Luo X,Ji R,Liu Q,Xiao X,Song W,An H,Li Y,Zhou J

Tue Dec 26 00:00:00 EST 2023

The m6A Reader YTHDF2 Promotes Bladder Cancer Progression by Suppressing RIG-I-Mediated Immune Response.

Cancer research

Zhang L,Li Y,Zhou L,Zhou H,Ye L,Ou T,Hong H,Zheng S,Zhou Z,Wu K,Yan Z,Thiery JP,Cui J,Wu S

Fri Jun 02 00:00:00 EDT 2023

The m6A Reader YTHDF2 Promotes Bladder Cancer Progression by Suppressing RIG-I-Mediated Immune Response.

Cancer research

Zhang L,Li Y,Zhou L,Zhou H,Ye L,Ou T,Hong H,Zheng S,Zhou Z,Wu K,Yan Z,Thiery JP,Cui J,Wu S

Fri Jun 02 00:00:00 EDT 2023

Prediction and Verification of Curcumin as a Potential Drug for Inhibition of PDCoV Replication in LLC-PK1 Cells.

International journal of molecular sciences

Wang X,Wang X,Zhang J,Shan Q,Zhu Y,Xu C,Wang J

Mon Mar 20 00:00:00 EDT 2023

The differential expression of toll like receptors and RIG-1 in the placenta of neonates with in utero infections.

Annals of diagnostic pathology

Rice M,Nicol A,Nuovo GJ

Wed Feb 01 00:00:00 EST 2023

ARIH1 inhibits influenza A virus replication and facilitates RIG-I dependent immune signaling by interacting with SQSTM1/p62.

Virology journal

Wang S,Li Z,Chen Y,Gao S,Qiao J,Liu H,Song H,Ao D,Sun X

Sat Apr 01 00:00:00 EDT 2023

Inhibition of LSD1 with Bomedemstat Sensitizes Small Cell Lung Cancer to Immune Checkpoint Blockade and T-Cell Killing.

Clinical cancer research : an official journal of the American Association for Cancer Research

Hiatt JB,Sandborg H,Garrison SM,Arnold HU,Liao SY,Norton JP,Friesen TJ,Wu F,Sutherland KD,Rienhoff HY,Martins R,Houghton AM,Srivastava S,MacPherson D

Fri Oct 14 00:00:00 EDT 2022

STING1 is essential for an RNA-virus triggered autophagy.

Autophagy

Zhang R,Qin X,Yang Y,Zhu X,Zhao S,Zhang Z,Su Q,Zhao Z,Yin X,Meng X,Zhang Z,Li Y

Fri Apr 01 00:00:00 EDT 2022

Peste des Petits Ruminants Virus Upregulates STING to Activate ATF6-Mediated Autophagy.

Journal of virology

Zhang R,Lin H,You Q,Zhang Z,Bai L,Chen F,Zhu X,Zhang Z,Li Y

Wed Oct 26 00:00:00 EDT 2022

Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDME.

eLife

Zhao Z,Li Q,Ashraf U,Yang M,Zhu W,Gu J,Chen Z,Gu C,Si Y,Cao S,Ye J

Tue Aug 16 00:00:00 EDT 2022

Activation of DDX58/RIG‑I suppresses the growth of tumor cells by inhibiting STAT3/CSE signaling in colon cancer.

International journal of oncology

Deng Y,Fu H,Han X,Li Y,Zhao W,Zhao X,Yu C,Guo W,Lei K,Wang T

Sat Oct 01 00:00:00 EDT 2022

Activation of DDX58/RIG‑I suppresses the growth of tumor cells by inhibiting STAT3/CSE signaling in colon cancer.

International journal of oncology

Deng Y,Fu H,Han X,Li Y,Zhao W,Zhao X,Yu C,Guo W,Lei K,Wang T

Sat Oct 01 00:00:00 EDT 2022

Activation of DDX58/RIG‑I suppresses the growth of tumor cells by inhibiting STAT3/CSE signaling in colon cancer.

International journal of oncology

Deng Y,Fu H,Han X,Li Y,Zhao W,Zhao X,Yu C,Guo W,Lei K,Wang T

Sat Oct 01 00:00:00 EDT 2022

Activation of DDX58/RIG‑I suppresses the growth of tumor cells by inhibiting STAT3/CSE signaling in colon cancer.

International journal of oncology

Deng Y,Fu H,Han X,Li Y,Zhao W,Zhao X,Yu C,Guo W,Lei K,Wang T

Sat Oct 01 00:00:00 EDT 2022

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Nature microbiology

Jiang Z,Wei F,Zhang Y,Wang T,Gao W,Yu S,Sun H,Pu J,Sun Y,Wang M,Tong Q,Gao C,Chang KC,Liu J

Thu Jul 01 00:00:00 EDT 2021

METTL3 regulates viral m6A RNA modification and host cell innate immune responses during SARS-CoV-2 infection.

Cell reports

Li N,Hui H,Bray B,Gonzalez GM,Zeller M,Anderson KG,Knight R,Smith D,Wang Y,Carlin AF,Rana TM

Tue May 11 00:00:00 EDT 2021

METTL3 regulates viral m6A RNA modification and host cell innate immune responses during SARS-CoV-2 infection.

Cell reports

Li N,Hui H,Bray B,Gonzalez GM,Zeller M,Anderson KG,Knight R,Smith D,Wang Y,Carlin AF,Rana TM

Tue May 11 00:00:00 EDT 2021

Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.

Cell reports

Shen Y,Tang K,Chen D,Hong M,Sun F,Wang S,Ke Y,Wu T,Sun R,Qian J,Du Y

Tue Jun 22 00:00:00 EDT 2021

Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Negatively Regulates Innate Immunity Against RNA Virus by Targeting RIG-I in Macrophages.

Frontiers in immunology

Zou Z,Li M,Zhou Y,Li J,Pan T,Lai L,Wang Q,Zhang L,Wang Q,Song Y,Zhang Y

Mon Sep 27 00:00:00 EDT 2021

Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Negatively Regulates Innate Immunity Against RNA Virus by Targeting RIG-I in Macrophages.

Frontiers in immunology

Zou Z,Li M,Zhou Y,Li J,Pan T,Lai L,Wang Q,Zhang L,Wang Q,Song Y,Zhang Y

Mon Sep 27 00:00:00 EDT 2021

Butyrate Reprograms Expression of Specific Interferon-Stimulated Genes.

Journal of virology

Chemudupati M,Kenney AD,Smith AC,Fillinger RJ,Zhang L,Zani A,Liu SL,Anderson MZ,Sharma A,Yount JS

Thu Jul 30 00:00:00 EDT 2020

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Nature communications

Wang C,Li Y,Yan S,Wang H,Shao X,Xiao M,Yang B,Qin G,Kong R,Chen R,Zhang N

Mon Jun 22 00:00:00 EDT 2020

RIG-I aggravates interstitial fibrosis via c-Myc-mediated fibroblast activation in UUO mice.

Journal of molecular medicine (Berlin, Germany)

Zhou Z,Ni J,Li J,Huo C,Miao N,Yin F,Cheng Q,Xu D,Xie H,Chen P,Zheng P,Zhang Y,Zhou L,Zhang W,Yu C,Liu J,Lu L

Wed Apr 01 00:00:00 EDT 2020

RIG-I aggravates interstitial fibrosis via c-Myc-mediated fibroblast activation in UUO mice.

Journal of molecular medicine (Berlin, Germany)

Zhou Z,Ni J,Li J,Huo C,Miao N,Yin F,Cheng Q,Xu D,Xie H,Chen P,Zheng P,Zhang Y,Zhou L,Zhang W,Yu C,Liu J,Lu L

Wed Apr 01 00:00:00 EDT 2020

RIG-I aggravates interstitial fibrosis via c-Myc-mediated fibroblast activation in UUO mice.

Journal of molecular medicine (Berlin, Germany)

Zhou Z,Ni J,Li J,Huo C,Miao N,Yin F,Cheng Q,Xu D,Xie H,Chen P,Zheng P,Zhang Y,Zhou L,Zhang W,Yu C,Liu J,Lu L

Wed Apr 01 00:00:00 EDT 2020

RIG-I aggravates interstitial fibrosis via c-Myc-mediated fibroblast activation in UUO mice.

Journal of molecular medicine (Berlin, Germany)

Zhou Z,Ni J,Li J,Huo C,Miao N,Yin F,Cheng Q,Xu D,Xie H,Chen P,Zheng P,Zhang Y,Zhou L,Zhang W,Yu C,Liu J,Lu L

Wed Apr 01 00:00:00 EDT 2020

The natural compound Cirsitakaoside enhances antiviral innate responses against vesicular stomatitis virus in vitro and in vivo.

International immunopharmacology

Di Q,Zhu H,Pu D,Zhao X,Li X,Ma X,Xiao W,Chen W

Tue Sep 01 00:00:00 EDT 2020

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Nature

Ringeard M,Marchand V,Decroly E,Motorin Y,Bennasser Y

Tue Jan 01 00:00:00 EST 2019

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Nature

Ringeard M,Marchand V,Decroly E,Motorin Y,Bennasser Y

Tue Jan 01 00:00:00 EST 2019

Ribosome biogenesis restricts innate immune responses to virus infection and DNA.

eLife

Bianco C,Mohr I

Mon Dec 16 00:00:00 EST 2019

The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.

Cell reports

Wang K,Ma H,Liu H,Ye W,Li Z,Cheng L,Zhang L,Lei Y,Shen L,Zhang F

Tue May 14 00:00:00 EDT 2019

Influenza Virus NS1 Protein-RNA Interactome Reveals Intron Targeting.

Journal of virology

Zhang L,Wang J,Muñoz-Moreno R,Kim M,Sakthivel R,Mo W,Shao D,Anantharaman A,García-Sastre A,Conrad NK,Fontoura BMA

Sat Dec 15 00:00:00 EST 2018

Influenza Virus NS1 Protein-RNA Interactome Reveals Intron Targeting.

Journal of virology

Zhang L,Wang J,Muñoz-Moreno R,Kim M,Sakthivel R,Mo W,Shao D,Anantharaman A,García-Sastre A,Conrad NK,Fontoura BMA

Sat Dec 15 00:00:00 EST 2018

Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Journal of virology

Burgess HM,Mohr I

Wed Aug 01 00:00:00 EDT 2018

Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Journal of virology

Burgess HM,Mohr I

Wed Aug 01 00:00:00 EDT 2018

Activation of the RLR/MAVS Signaling Pathway by the L Protein of Mopeia Virus.

Journal of virology

Zhang LK,Xin QL,Zhu SL,Wan WW,Wang W,Xiao G

Tue Nov 15 00:00:00 EST 2016

Activation of the RLR/MAVS Signaling Pathway by the L Protein of Mopeia Virus.

Journal of virology

Zhang LK,Xin QL,Zhu SL,Wan WW,Wang W,Xiao G

Tue Nov 15 00:00:00 EST 2016

The ORF3 Protein of Genotype 1 Hepatitis E Virus Suppresses TLR3-induced NF-κB Signaling via TRADD and RIP1.

Scientific reports

He M,Wang M,Huang Y,Peng W,Zheng Z,Xia N,Xu J,Tian D

Wed Jun 08 00:00:00 EDT 2016

The ORF3 Protein of Genotype 1 Hepatitis E Virus Suppresses TLR3-induced NF-κB Signaling via TRADD and RIP1.

Scientific reports

He M,Wang M,Huang Y,Peng W,Zheng Z,Xia N,Xu J,Tian D

Wed Jun 08 00:00:00 EDT 2016

The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Functions as a Novel Cytosolic Pathogen-Associated Molecular Pattern To Promote Beta Interferon Induction via a Toll-Like-Receptor-Related TRAF3-Independent Mechanism.

mBio

Wang Y,Liu L

Tue Feb 09 00:00:00 EST 2016

mBio

Wang Y,Liu L

Tue Feb 09 00:00:00 EST 2016

Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins.

The Journal of general virology

Zhang W,Zhang L,Zan Y,Du N,Yang Y,Tien P

Thu Jan 01 00:00:00 EST 2015

Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins.

The Journal of general virology

Zhang W,Zhang L,Zan Y,Du N,Yang Y,Tien P

Thu Jan 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Antiviral innate immune response receptor RIG-I; ATP-dependent RNA helicase DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD-box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DEAD/H box polypeptide RIG-I; probable ATP-dependent RNA helicase DDX58; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; retinoic acid-inducible gene-I; RIG-1; RIG-I; RIG-I-like receptor 1; RLR-1; RNA helicase RIG-I; RNA sensor RIG-I

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Gene Aliases: 6430573D20Rik; C330021E21; DDX58; RIG-I; RIGI; RLR-1; SGMRT2

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UniProt ID: (Human) O95786, (Mouse) Q6Q899

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Entrez Gene ID: (Human) 23586, (Mouse) 230073

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Function(s)

double-stranded DNA binding double-stranded RNA binding single-stranded RNA binding helicase activity protein binding ATP binding zinc ion binding identical protein binding nucleotide binding nucleic acid binding RNA binding hydrolase activity hydrolase activity, acting on acid anhydrides metal ion binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DDX58 Polyclonal Antibody

DDX58 Antibody (20566-1-AP) in ICC/IF

Product Details

20566-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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