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DEFB104A Antibody (500-P268BT-25UG) in ELISA
Sandwich ELISA: To detect hBD-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of DEFB104A Polyclonal Antibody, Biotin (Product # 500-P268BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech DEFB104A Polyclonal Antibody (Product # 500-P268-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-4.
Product Details
500-P268BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BD-4
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930162
Product Specific Information
AA Sequence of recombinant protein: EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Humn BD-4. Anti-Human BD-4-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBD-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-4 (500-P268) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-4.
Western Blot: To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P268BT-1MG will be provided as 2 x 500 µg
Target Information
Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104A, represents the more centromeric copy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BD-4; Beta-defensin 104; Beta-defensin 4; Defensin, beta 104; defensin, beta 104A; defensin, beta 4
Gene Aliases: BD-4; DEFB-4; DEFB104; DEFB104A; DEFB104B; DEFB4; hBD-4
UniProt ID: (Human) Q8WTQ1
Entrez Gene ID: (Human) 140596
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