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DEFB121 Antibody (PA5-102387) in ICC/IF
Immunofluorescent analysis of DEFB121 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DEFB121 polyclonal antibody (Product # PA5-102387) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102387
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DEFB121(Accession Q5J5C9), corresponding to amino acid residues V39-V76.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815920
Product Specific Information
Antibody detects endogenous levels of total DEFB121.
Target Information
Beta-defensins (also designated BDs, or hBDs in human) are small cationic peptides with broad-spectrum antimicrobial activity against a variety of enveloped viruses, fungi and bacteria. Produced in mucosal epithelia and neutrophils of several species, beta-defensins are developmentally regulated. The family of beta-defensin proteins share a common defensin-motif that is characterized by multiple cysteine residues and a highly conserved tertiary structure. Besides playing a significant role in host immune defense, many beta-defensins also are involved in sperm maturation and capacitation. beta-defensin 121 is a 76 amino acid secreted protein that most likely contains a signal peptide sequence that requires cleavage by proteolytic enzymes in order to become biologically active. While expressed at low levels in testis, beta-defensin 121 is abundantly expressed in epididymal caput and corpus and, unlike other beta-defensins, it does not appear to be regulated by androgens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-defensin 121; Beta-defensin 21; DB121; DEFB-21; Defensin, beta 121; defensin, beta 21
Gene Aliases: DEFB121; DEFB21; ESC42RELC
UniProt ID: (Human) Q5J5C9
Entrez Gene ID: (Human) 245934
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