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DIABLO Polyclonal Antibody

DIABLO Antibody in Immunocytochemistry (ICC/IF)

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DIABLO Antibody (10434-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIABLO antibody (Product # 10434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DIABLO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DIABLO Antibody in Flow Cytometry (Flow)

DIABLO Antibody in Immunoprecipitation (IP)

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Product Details

10434-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:200

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DIABLO Fusion Protein Ag0696 (1-239 aa encoded by BC011909)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes all the three isoforms (27 kDa and 22 kDa) of DIABLO. The highest expression was in the testis, adrenal gland, and ears.

Immunogen sequence: MAALKSWLS RSVTSFFRYR QCLCVPVVAN FKKRCFSELI RPWHKTVTIG FGVTLCAVPI AQKSEPHSLS SEALMRRAVS LVTDSTSTFL SQTTYALIEA ITEYTKAVYT LTSLYRQYTS LLGKMNSEEE DEVWQVIIGA RAEMTSKHQE YLKLETTWMT AVGLSEMAAE AAYQTGADQA SITARNHIQL VKLQVEEVHQ LSRKAETKLA EAQIEELRQK TQEEGEERAE SEQEAYLRED (1-239 aa encoded by BC011909)

Target Information

DIABLO (smac), a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms. Smac/DIABLO has also been found to play a key role in regulating the sensitization of cancer cells to apoptosis (both immune and drug-induced).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism.

European journal of medicinal chemistry

Lin H,Han H,Yang M,Wen Z,Chen Q,Ma Y,Wang X,Wang C,Yin T,Wang X,Lu G,Chen H,Qi J,Yang Y

Sun Mar 05 00:00:00 EST 2023

ROS-Triggered Self-Assembled Nanoparticles Based on a Chemo-Sonodynamic Combinational Therapy Strategy for the Noninvasive Elimination of Hypoxic Tumors.

ACS applied materials & interfaces

Li Y,Lin L,Xie J,Wei L,Xiong S,Yu K,Zhang B,Wang S,Li Z,Tang Y,Chen G,Li Z,Yu Z,Wang X

Wed Mar 29 00:00:00 EDT 2023

Glutathione Might Attenuate Arsenic-Induced Liver Injury by Modulating the Foxa2-XIAP Axis to Reduce Oxidative Stress and Mitochondrial Apoptosis.

Biological trace element research

Zhang H,Jin B,Liu L,Li H,Zheng X,Li M,He R,Wang K

Wed Nov 01 00:00:00 EDT 2023

The Photoperiod Regulates Granulosa Cell Apoptosis through the FSH-Nodal/ALK7 Signaling Pathway in Phodopus sungorus.

Animals : an open access journal from MDPI

Qi Y,Xue HL,Zheng YJ,Yin YF,Xu WL,Xu JH,Wu M,Chen L,Xu LX

Fri Dec 16 00:00:00 EST 2022

Botrychium schaffneri Underw. extract acts via DIABLO to induce apoptosis and inhibit proliferation of non-small cell lung carcinoma in vitro and in vivo.

Annals of translational medicine

Liu YJ,Zhang YW,Zheng SL,Zheng XC,Ding HY,Xin WX,Sun J,Li L,Huang P

Mon Nov 01 00:00:00 EDT 2021

Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.

Chinese medicine

Lin HY,Han HW,Wang YS,He DL,Sun WX,Feng L,Wen ZL,Yang MK,Lu GH,Wang XM,Qi JL,Yang YH

Wed Apr 13 00:00:00 EDT 2022

Mechanisms underlying the protective effect of tannic acid against arsenic trioxide‑induced cardiotoxicity in rats: Potential involvement of mitochondrial apoptosis.

Molecular medicine reports

Xue Y,Li M,Xue Y,Jin W,Han X,Zhang J,Chu X,Li Z,Chu L

Tue Dec 01 00:00:00 EST 2020

Resveratrol ameliorates podocyte damage in diabetic mice via SIRT1/PGC-1α mediated attenuation of mitochondrial oxidative stress.

Journal of cellular physiology

Zhang T,Chi Y,Kang Y,Lu H,Niu H,Liu W,Li Y

Mon Apr 01 00:00:00 EDT 2019

Resveratrol ameliorates podocyte damage in diabetic mice via SIRT1/PGC-1α mediated attenuation of mitochondrial oxidative stress.

Journal of cellular physiology

Zhang T,Chi Y,Kang Y,Lu H,Niu H,Liu W,Li Y

Mon Apr 01 00:00:00 EDT 2019

Resveratrol Reduces Oxidative Stress and Apoptosis in Podocytes via Sir2-Related Enzymes, Sirtuins1 (SIRT1)/Peroxisome Proliferator-Activated Receptor γ Co-Activator 1α (PGC-1α) Axis.

Medical science monitor : international medical journal of experimental and clinical research

Zhang T,Chi Y,Ren Y,Du C,Shi Y,Li Y

Fri Feb 15 00:00:00 EST 2019

Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.

Oncology letters

Duan H,Wang R,Yan X,Liu H,Zhang Y,Mu D,Han J,Li X

Fri Dec 01 00:00:00 EST 2017

Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.

Oncology letters

Duan H,Wang R,Yan X,Liu H,Zhang Y,Mu D,Han J,Li X

Fri Dec 01 00:00:00 EST 2017

Cathelicidin PR-39 peptide inhibits hypoxia/reperfusion-induced kidney cell apoptosis by suppression of the endoplasmic reticulum-stress pathway.

Acta biochimica et biophysica Sinica

Liu J,Xue W,Xiang H,Zheng J,Zhao Y,Jiao L,Jiao Z

Mon Aug 01 00:00:00 EDT 2016

Cathelicidin PR-39 peptide inhibits hypoxia/reperfusion-induced kidney cell apoptosis by suppression of the endoplasmic reticulum-stress pathway.

Acta biochimica et biophysica Sinica

Liu J,Xue W,Xiang H,Zheng J,Zhao Y,Jiao L,Jiao Z

Mon Aug 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Diablo homolog, mitochondrial; Diablo IAP-binding mitochondrial protein; diablo, IAP-binding mitochondrial protein; diablo-like protein; DIABLO-S; direct IAP binding protein with low PI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; hCG_1782202; Second mitochondria-derived activator of caspase; Second mitochondria-derived activator of caspases; SMAC; SMAC3

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Gene Aliases: 0610041G12Rik; 1700006L01Rik; AU040403; DFNA64; DIABLO; SMAC

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UniProt ID: (Human) Q9NR28, (Mouse) Q9JIQ3

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Entrez Gene ID: (Human) 56616, (Mouse) 66593, (Rat) 288753

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Function(s)

protein binding

Process(es)

apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process intrinsic apoptotic signaling pathway in response to oxidative stress activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c positive regulation of apoptotic process neuron apoptotic process intrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway via death domain receptors

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DIABLO Polyclonal Antibody

DIABLO Antibody (10434-1-AP) in ICC/IF

Product Details

10434-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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