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Invitrogen
DIABLO Recombinant Rabbit Monoclonal Antibody (RM271)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Product Details
MA5-27979
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM271
Immunogen
A peptide corresponding to the C-terminus of human Smac (Diablo homolog, mitochondrial)
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2744994
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
DIABLO (smac), a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms. Smac/DIABLO has also been found to play a key role in regulating the sensitization of cancer cells to apoptosis (both immune and drug-induced).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Diablo homolog, mitochondrial; Diablo IAP-binding mitochondrial protein; diablo, IAP-binding mitochondrial protein; diablo-like protein; DIABLO-S; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; hCG_1782202; second mitochondria-derived activator of caspase; Second mitochondria-derived activator of caspases; SMAC; SMAC3
Gene Aliases: DFNA64; DIABLO; SMAC
UniProt ID: (Human) Q9NR28
Entrez Gene ID: (Human) 56616
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to oxidative stress
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
positive regulation of apoptotic process
neuron apoptotic process
intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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