Proteintech

DICER1 Polyclonal Antibody

DICER1 Antibody in Immunocytochemistry (ICC/IF)

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DICER1 Antibody (20567-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 20567-1-AP (DICER1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

DICER1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DICER1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

DICER1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20567-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:25

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.57 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to DICER1. The calculated molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 has isoforms with molecular weight 219 kDa and 93 kDa.

Target Information

Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Circular RNA hsa_circ_0007444 inhibits ovarian cancer progression through miR-23a-3p/DICER1 axis.

Acta biochimica et biophysica Sinica

Zhang M,Sun Y,Xu H,Shi Y,Shen R,Teng F,Xu J,Jia X

Thu Apr 13 00:00:00 EDT 2023

ADAR1 inhibits adipogenesis and obesity by interacting with Dicer to promote the maturation of miR-155-5P.

Journal of cell science

Yu Z,Luo R,Li Y,Li X,Yang Z,Peng J,Huang K

Tue Mar 01 00:00:00 EST 2022

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Journal of medicinal chemistry

Peng T,He Y,Wang T,Yu J,Ma X,Zhou Z,Sheng Y,Li L,Peng H,Li S,Zou J,Yuan Y,Zhao Y,Shi H,Li F,Liu W,Hu K,Lu X,Zhang G,Wang F

Thu Aug 25 00:00:00 EDT 2022

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Journal of medicinal chemistry

Peng T,He Y,Wang T,Yu J,Ma X,Zhou Z,Sheng Y,Li L,Peng H,Li S,Zou J,Yuan Y,Zhao Y,Shi H,Li F,Liu W,Hu K,Lu X,Zhang G,Wang F

Thu Aug 25 00:00:00 EDT 2022

DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity.

Nucleic acids research

Yan A,Xiong J,Zhu J,Li X,Xu S,Feng X,Ke X,Wang Z,Chen Y,Wang HW,Zhang MQ,Kee K

Fri Oct 28 00:00:00 EDT 2022

HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.

Nucleic acids research

Liu X,Yang YY,Wang Y

Fri Jul 08 00:00:00 EDT 2022

HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.

Nucleic acids research

Liu X,Yang YY,Wang Y

Fri Jul 08 00:00:00 EDT 2022

The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells.

Viruses

Xu J,Kong J,Lyu B,Wang X,Qian Q,Zhou X,Qiu Y

Thu Jan 21 00:00:00 EST 2021

Amyloid peptide exerts a rapid induction of Dicer1 protein in neuron via reducing phosphorylation.

Neurochemistry international

Wang Y,Xiu X,Wu S

Wed Dec 01 00:00:00 EST 2021

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Neuron

Li X,Zhang J,Li D,He C,He K,Xue T,Wan L,Zhang C,Liu Q

Wed Mar 17 00:00:00 EDT 2021

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

Journal of medicinal chemistry

Zhou Z,Li Y,Ma X,Cao B,Peng T,Sheng Y,Peng H,Li R,Cao Y,Xi R,Li F,Wang M,Sun H,Zhang G,Zhang H,Hu K,Xiao W,Wang F

Thu Jun 10 00:00:00 EDT 2021

YB1 regulates miR-205/200b-ZEB1 axis by inhibiting microRNA maturation in hepatocellular carcinoma.

Cancer communications (London, England)

Liu X,Chen D,Chen H,Wang W,Liu Y,Wang Y,Duan C,Ning Z,Guo X,Otkur W,Liu J,Qi H,Liu X,Lin A,Xia T,Liu HX,Piao HL

Thu Jul 01 00:00:00 EDT 2021

YB1 regulates miR-205/200b-ZEB1 axis by inhibiting microRNA maturation in hepatocellular carcinoma.

Cancer communications (London, England)

Liu X,Chen D,Chen H,Wang W,Liu Y,Wang Y,Duan C,Ning Z,Guo X,Otkur W,Liu J,Qi H,Liu X,Lin A,Xia T,Liu HX,Piao HL

Thu Jul 01 00:00:00 EDT 2021

Dietary daidzein inhibits hepatitis C virus replication by decreasing microRNA-122 levels.

Virus research

He Y,Huang M,Tang C,Yue Y,Liu X,Zheng Z,Dong H,Liu D

Tue Jun 01 00:00:00 EDT 2021

RNA structure probing reveals the structural basis of Dicer binding and cleavage.

Nature communications

Luo QJ,Zhang J,Li P,Wang Q,Zhang Y,Roy-Chaudhuri B,Xu J,Kay MA,Zhang QC

Mon Jun 07 00:00:00 EDT 2021

The Capsid Protein of Semliki Forest Virus Antagonizes RNA Interference in Mammalian Cells.

Journal of virology

Qian Q,Zhou H,Shu T,Mu J,Fang Y,Xu J,Li T,Kong J,Qiu Y,Zhou X

Fri Jan 17 00:00:00 EST 2020

Differential roles of human PUS10 in miRNA processing and tRNA pseudouridylation.

Nature chemical biology

Song J,Zhuang Y,Zhu C,Meng H,Lu B,Xie B,Peng J,Li M,Yi C

Sat Feb 01 00:00:00 EST 2020

Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence.

Cell death & disease

Wang MY,Chen DP,Qi B,Li MY,Zhu YY,Yin WJ,He L,Yu Y,Li ZY,Lin L,Yang F,Lin ZR,Liu JQ

Mon Jun 03 00:00:00 EDT 2019

Imbalance of a KLF4-miR-7 auto-regulatory feedback loop promotes prostate cancer cell growth by impairing microRNA processing.

American journal of cancer research

Wei LZ,Wang YQ,Chang YL,An N,Wang X,Zhou PJ,Zhu HH,Fang YX,Gao WQ

Thu Oct 01 00:00:00 EDT 2020

Imbalance of a KLF4-miR-7 auto-regulatory feedback loop promotes prostate cancer cell growth by impairing microRNA processing.

American journal of cancer research

Wei LZ,Wang YQ,Chang YL,An N,Wang X,Zhou PJ,Zhu HH,Fang YX,Gao WQ

Thu Oct 01 00:00:00 EDT 2020

LncRNA UCA1 is necessary for TGF-β-induced epithelial-mesenchymal transition and stemness via acting as a ceRNA for Slug in glioma cells.

FEBS open bio

Li Z,Liu H,Zhong Q,Wu J,Tang Z

Thu Nov 01 00:00:00 EDT 2018

LncRNA UCA1 is necessary for TGF-β-induced epithelial-mesenchymal transition and stemness via acting as a ceRNA for Slug in glioma cells.

FEBS open bio

Li Z,Liu H,Zhong Q,Wu J,Tang Z

Thu Nov 01 00:00:00 EDT 2018

Iridoids with Genipin Stem Nucleus Inhibit Lipopolysaccharide-Induced Inflammation and Oxidative Stress by Blocking the NF-κB Pathway in Polycystic Ovary Syndrome.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zuo T,Zhu M,Xu W,Wang Z,Song H

Mon Jan 22 00:00:00 EST 2018

Iridoids with Genipin Stem Nucleus Inhibit Lipopolysaccharide-Induced Inflammation and Oxidative Stress by Blocking the NF-κB Pathway in Polycystic Ovary Syndrome.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zuo T,Zhu M,Xu W,Wang Z,Song H

Mon Jan 22 00:00:00 EST 2018

The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.

Scientific reports

Wang Y,Chen F,Yang Z,Zhao M,Zhang S,Gao Y,Feng J,Yang G,Zhang W,Ye L,Zhang X

Thu May 18 00:00:00 EDT 2017

The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.

Scientific reports

Wang Y,Chen F,Yang Z,Zhao M,Zhang S,Gao Y,Feng J,Yang G,Zhang W,Ye L,Zhang X

Thu May 18 00:00:00 EDT 2017

Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.

Nature communications

Xu C,Zhang Y,Wang Q,Xu Z,Jiang J,Gao Y,Gao M,Kang J,Wu M,Xiong J,Ji K,Yuan W,Wang Y,Liu H

Fri Nov 04 00:00:00 EDT 2016

Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.

Nature communications

Xu C,Zhang Y,Wang Q,Xu Z,Jiang J,Gao Y,Gao M,Kang J,Wu M,Xiong J,Ji K,Yuan W,Wang Y,Liu H

Fri Nov 04 00:00:00 EDT 2016

Dicer suppresses MMP-2-mediated invasion and VEGFA-induced angiogenesis and serves as a promising prognostic biomarker in human clear cell renal cell carcinoma.

Oncotarget

Chen YS,Meng F,Li HL,Liu QH,Hou PF,Bai J,Zheng JN

Tue Dec 20 00:00:00 EST 2016

Dicer suppresses MMP-2-mediated invasion and VEGFA-induced angiogenesis and serves as a promising prognostic biomarker in human clear cell renal cell carcinoma.

Oncotarget

Chen YS,Meng F,Li HL,Liu QH,Hou PF,Bai J,Zheng JN

Tue Dec 20 00:00:00 EST 2016

Cancer-associated somatic DICER1 hotspot mutations cause defective miRNA processing and reverse-strand expression bias to predominantly mature 3p strands through loss of 5p strand cleavage.

The Journal of pathology

Anglesio MS,Wang Y,Yang W,Senz J,Wan A,Heravi-Moussavi A,Salamanca C,Maines-Bandiera S,Huntsman DG,Morin GB

Fri Feb 01 00:00:00 EST 2013

Cancer-associated somatic DICER1 hotspot mutations cause defective miRNA processing and reverse-strand expression bias to predominantly mature 3p strands through loss of 5p strand cleavage.

The Journal of pathology

Anglesio MS,Wang Y,Yang W,Senz J,Wan A,Heravi-Moussavi A,Salamanca C,Maines-Bandiera S,Huntsman DG,Morin GB

Fri Feb 01 00:00:00 EST 2013

Cancer-associated somatic DICER1 hotspot mutations cause defective miRNA processing and reverse-strand expression bias to predominantly mature 3p strands through loss of 5p strand cleavage.

The Journal of pathology

Anglesio MS,Wang Y,Yang W,Senz J,Wan A,Heravi-Moussavi A,Salamanca C,Maines-Bandiera S,Huntsman DG,Morin GB

Fri Feb 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: DCR; DCR-1; dicer 1 ribonuclease III; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; helicase-moi

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Gene Aliases: DCR1; DICER; DICER1; Dicer1e; HERNA; K12H4.8-LIKE; KIAA0928; MNG1; RMSE2

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UniProt ID: (Human) Q9UPY3

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Entrez Gene ID: (Human) 23405

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Function(s)

double-stranded RNA binding helicase activity endoribonuclease activity ribonuclease III activity deoxyribonuclease I activity protein binding ATP binding protein domain specific binding siRNA binding metal ion binding pre-miRNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter apoptotic DNA fragmentation negative regulation of Schwann cell proliferation positive regulation of Schwann cell differentiation nerve development production of siRNA involved in RNA interference targeting of mRNA for destruction involved in RNA interference gene silencing by RNA pre-miRNA processing positive regulation of myelination peripheral nervous system myelin formation conversion of ds siRNA to ss siRNA involved in RNA interference siRNA loading onto RISC involved in RNA interference production of miRNAs involved in gene silencing by miRNA miRNA loading onto RISC involved in gene silencing by miRNA conversion of ds siRNA to ss siRNA neuron projection morphogenesis RNA phosphodiester bond hydrolysis, endonucleolytic

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DICER1 Polyclonal Antibody

DICER1 Antibody (20567-1-AP) in ICC/IF

Product Details

20567-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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