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DIO1 Polyclonal Antibody

DIO1 Antibody in Immunocytochemistry (ICC/IF)

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DIO1 Antibody (11790-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 11790-1-AP (DIO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DIO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DIO1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11790-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DIO1 Fusion Protein Ag2410 (1-161 aa encoded by BC017955)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.45 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGLPQPGLW LKRLWVLLEV AVHVVVGKVL LILFPDRVKR NILAMGEKTG MTRNPHFSHD NWIPTFFSTQ YFWFVLKVRW QRLEDTTELG GLAPNCPVVR LSGQRCNIWE FMQGNRPLVL NFGSCTUPSF MFKFDQFKRL IEDFSSIADF LVIYIEEAHA SG (1-161 aa encoded by BC017955)

Target Information

The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some, but not all, isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Selenoprotein Gene mRNA Expression Evaluation During Renal Ischemia-Reperfusion Injury in Rats and Ebselen Intervention Effects.

Biological trace element research

Wu Y,Shi H,Xu Y,Wen R,Gong M,Hong G,Xu S

Sat Apr 01 00:00:00 EDT 2023

Role of aerobic exercise in ameliorating NASH: Insights into the hepatic thyroid hormone signaling and circulating thyroid hormones.

Frontiers in endocrinology

Liu Q,Li H,He W,Zhao Q,Huang C,Wang Q,Zheng Z,Zhang X,Shi X,Li X

Tue Jan 10 00:00:00 EST 2023

Effect of Preeclampsia on Ultrastructure of Thyroid Gland, Hepatic Type 1 Iodothyronine Deiodinase, and Thyroid Hormone Levels in Rats.

BioMed research international

Liu Y,Xu Z,Li Y,Jiang W,Lan M,Xie X,Wang Y

Mon Oct 25 00:00:00 EDT 2021

Role of diets and exercise in ameliorating obesity-related hepatic steatosis: Insights at the microRNA-dependent thyroid hormone synthesis and action.

Life sciences

Xia SF,Jiang YY,Qiu YY,Huang W,Wang J

Sat Feb 01 00:00:00 EST 2020

Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.

Scientific reports

Chellan B,Zhao L,Landeche M,Carmean CM,Dumitrescu AM,Sargis RM

Thu Oct 29 00:00:00 EDT 2020

Rno-miR-224-5p contributes to 2,2',4,4'-tetrabromodiphenyl ether-induced low triiodothyronine in rats by targeting deiodinases.

Chemosphere

Wang C,Zhu J,Zhang Z,Chen H,Ji M,Chen C,Hu Y,Yu Y,Xia R,Shen J,Gong X,Wang SL

Fri May 01 00:00:00 EDT 2020

Rno-miR-224-5p contributes to 2,2',4,4'-tetrabromodiphenyl ether-induced low triiodothyronine in rats by targeting deiodinases.

Chemosphere

Wang C,Zhu J,Zhang Z,Chen H,Ji M,Chen C,Hu Y,Yu Y,Xia R,Shen J,Gong X,Wang SL

Fri May 01 00:00:00 EDT 2020

Myricetin alleviated hepatic steatosis by acting on microRNA-146b/thyroid hormone receptor b pathway in high-fat diet fed C57BL/6J mice.

Food & function

Xia SF,Qiu YY,Chen LM,Jiang YY,Huang W,Xie ZX,Tang X,Sun J

Wed Mar 20 00:00:00 EDT 2019

Reduced Skeletal Muscle Protein Turnover and Thyroid Hormone Metabolism in Adaptive Thermogenesis That Facilitates Body Fat Recovery During Weight Regain.

Frontiers in endocrinology

Calonne J,Isacco L,Miles-Chan J,Arsenijevic D,Montani JP,Guillet C,Boirie Y,Dulloo AG

Tue Feb 25 00:00:00 EST 2020

Environmental Enrichment Induces Pericyte and IgA-Dependent Wound Repair and Lifespan Extension in a Colon Tumor Model.

Cell reports

Bice BD,Stephens MR,Georges SJ,Venancio AR,Bermant PC,Warncke AV,Affolter KE,Hidalgo JR,Angus-Hill ML

Tue Apr 25 00:00:00 EDT 2017

Environmental Enrichment Induces Pericyte and IgA-Dependent Wound Repair and Lifespan Extension in a Colon Tumor Model.

Cell reports

Bice BD,Stephens MR,Georges SJ,Venancio AR,Bermant PC,Warncke AV,Affolter KE,Hidalgo JR,Angus-Hill ML

Tue Apr 25 00:00:00 EDT 2017

Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC).

The Journal of biological chemistry

Srivastava J,Robertson CL,Gredler R,Siddiq A,Rajasekaran D,Akiel MA,Emdad L,Mas V,Mukhopadhyay ND,Fisher PB,Sarkar D

Fri Jun 19 00:00:00 EDT 2015

Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC).

The Journal of biological chemistry

Srivastava J,Robertson CL,Gredler R,Siddiq A,Rajasekaran D,Akiel MA,Emdad L,Mas V,Mukhopadhyay ND,Fisher PB,Sarkar D

Fri Jun 19 00:00:00 EDT 2015

Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC).

The Journal of biological chemistry

Srivastava J,Robertson CL,Gredler R,Siddiq A,Rajasekaran D,Akiel MA,Emdad L,Mas V,Mukhopadhyay ND,Fisher PB,Sarkar D

Fri Jun 19 00:00:00 EDT 2015

Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC).

The Journal of biological chemistry

Srivastava J,Robertson CL,Gredler R,Siddiq A,Rajasekaran D,Akiel MA,Emdad L,Mas V,Mukhopadhyay ND,Fisher PB,Sarkar D

Fri Jun 19 00:00:00 EDT 2015

Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding.

Frontiers in physiology

De Andrade PB,Neff LA,Strosova MK,Arsenijevic D,Patthey-Vuadens O,Scapozza L,Montani JP,Ruegg UT,Dulloo AG,Dorchies OM

Wed Oct 07 00:00:00 EDT 2015

Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding.

Frontiers in physiology

De Andrade PB,Neff LA,Strosova MK,Arsenijevic D,Patthey-Vuadens O,Scapozza L,Montani JP,Ruegg UT,Dulloo AG,Dorchies OM

Wed Oct 07 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 5DI; DIOI; hDido1; iodothyronine deiodinase type 1; thyroxine 5'-deiodinase; thyroxine deiodinase; thyroxine deiodinase type I (selenoprotein); thyroxine deiodinase, type I; Type 1 DI; Type I iodothyronine deiodinase; Type-I 5'-deiodinase

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Gene Aliases: 5DI; D1; DIO1; ITDI1; TXDI1

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UniProt ID: (Human) P49895, (Mouse) Q61153, (Rat) P24389

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Entrez Gene ID: (Human) 1733, (Mouse) 13370, (Rat) 25430

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Function(s)

thyroxine 5'-deiodinase activity selenium binding oxidoreductase activity

Process(es)

hormone biosynthetic process oxidation-reduction process thyroid hormone generation generation of precursor metabolites and energy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DIO1 Polyclonal Antibody

DIO1 Antibody (11790-1-AP) in ICC/IF

Product Details

11790-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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