Proteintech

DIS3 Polyclonal Antibody

DIS3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

DIS3 Antibody (14689-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14689-1-AP (DIS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DIS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

14689-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fruit fly, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DIS3 Fusion Protein Ag6117 (607-958 aa encoded by BC056143)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TTSLRGLNK LAKILKKRRI EKGALTLSSP EVRFHMDSET HDPIDLQTKE LRETNSMVEE FMLLANISVA KKIHEEFSEH ALLRKHPAPP PSNYEILVKA ARSRNLEIKT DTAKSLAESL DQAESPTFPY LNTLLRILAT RCMMQAVYFC SGMDNDFHHY GLASPIYTHF TSPIRRYADV IVHRLLAVAI GADCTYPELT DKHKLADICK NLNFRHKMAQ YAQRASVAFH TQLFFKSKGI VSEEAYILFV RKNAIVVLIP KYGLEGTVFF EEKDKPNPQL IYDDEIPSLK IEDTVFHVFD KVKVKIMLDS SNLQHQKIRM SLVEPQIPGI SIPTDTSNMD LNGPKKKKMK LGK (607-958 aa encoded by BC056143)

Target Information

The exosome is involved in a multitude of cellular RNA processing and degradation events. DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. DIS3 localizes to both the cytoplasm and the nucleus and contains one PINc domain. It is widely expressed with highest expression in testis and is required for processing of 7S pre-RNA into a mature nuclear complex and, ultimately, for proper mitotic progression. Abnormal expression levels of DIS3 may be associated with colon cancer, suggesting a role for DIS3 in tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication.

Life science alliance

Moll T,Odon V,Harvey C,Collins MO,Peden A,Franklin J,Graves E,Marshall JN,Dos Santos Souza C,Zhang S,Castelli L,Hautbergue G,Azzouz M,Gordon D,Krogan N,Ferraiuolo L,Snyder MP,Shaw PJ,Rehwinkel J,Cooper-Knock J

Sun Jan 01 00:00:00 EST 2023

Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction.

International journal of biological sciences

Gao X,Li H,Zhang W,Wang X,Sun H,Cao Y,Zhao Y,Ji H,Yang F,Ma W,Liu Y,Yang B,Cai B

Thu May 12 00:00:00 EDT 2022

Loss of ribonuclease DIS3 hampers genome integrity in myeloma by disrupting DNA:RNA hybrid metabolism.

The EMBO journal

Gritti I,Basso V,Rinchai D,Corigliano F,Pivetti S,Gaviraghi M,Rosano D,Mazza D,Barozzi S,Roncador M,Parmigiani G,Legube G,Parazzoli D,Cittaro D,Bedognetti D,Mondino A,Segalla S,Tonon G

Thu Nov 17 00:00:00 EST 2022

RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis.

Nucleic acids research

Mehta C,Fraga de Andrade I,Matson DR,Dewey CN,Bresnick EH

Mon Sep 20 00:00:00 EDT 2021

The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.

The EMBO journal

Kawabe Y,Mori K,Yamashita T,Gotoh S,Ikeda M

Thu Oct 01 00:00:00 EDT 2020

Collaborative Control of Cell Cycle Progression by the RNA Exonuclease Dis3 and Ras Is Conserved Across Species.

Genetics

Snee MJ,Wilson WC,Zhu Y,Chen SY,Wilson BA,Kseib C,O'Neal J,Mahajan N,Tomasson MH,Arur S,Skeath JB

Wed Jun 01 00:00:00 EDT 2016

Collaborative Control of Cell Cycle Progression by the RNA Exonuclease Dis3 and Ras Is Conserved Across Species.

Genetics

Snee MJ,Wilson WC,Zhu Y,Chen SY,Wilson BA,Kseib C,O'Neal J,Mahajan N,Tomasson MH,Arur S,Skeath JB

Wed Jun 01 00:00:00 EDT 2016

DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs.

Molecular cell

Macias S,Cordiner RA,Gautier P,Plass M,Cáceres JF

Thu Dec 17 00:00:00 EST 2015

A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.

Nature

Chang HM,Triboulet R,Thornton JE,Gregory RI

Thu May 09 00:00:00 EDT 2013

A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.

Nature

Chang HM,Triboulet R,Thornton JE,Gregory RI

Thu May 09 00:00:00 EDT 2013

Genomic profiling of rectal adenoma and carcinoma by array-based comparative genomic hybridization.

BMC medical genomics

Shi ZZ,Zhang YM,Shang L,Hao JJ,Zhang TT,Wang BS,Liang JW,Chen X,Zhang Y,Wang GQ,Wang MR,Zhang Y

Fri Nov 16 00:00:00 EST 2012

Genomic profiling of rectal adenoma and carcinoma by array-based comparative genomic hybridization.

BMC medical genomics

Shi ZZ,Zhang YM,Shang L,Hao JJ,Zhang TT,Wang BS,Liang JW,Chen X,Zhang Y,Wang GQ,Wang MR,Zhang Y

Fri Nov 16 00:00:00 EST 2012

Bioinformatics

Protein Aliases: bA555G22.1; DIS3 exosome endoribonuclease and 3'-5' exoribonuclease; DIS3 mitotic control homolog; Exosome complex exonuclease RRP44; exosome component 11; mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA-processing protein 44; RP11-342J4.3

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Gene Aliases: 2810028N01Rik; DIS3; dis3p; EXOSC11; KIAA1008; RGD1304646; RRP44

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UniProt ID: (Human) Q9Y2L1, (Mouse) Q9CSH3

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Entrez Gene ID: (Human) 22894, (Mouse) 72662, (Rat) 306103

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Function(s)

3'-5'-exoribonuclease activity RNA binding endonuclease activity guanyl-nucleotide exchange factor activity protein binding nuclease activity exonuclease activity 5'-3' exoribonuclease activity bidentate ribonuclease III activity hydrolase activity exoribonuclease

Process(es)

rRNA processing mRNA catabolic process rRNA catabolic process regulation of mRNA stability positive regulation of GTPase activity exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay CUT catabolic process RNA phosphodiester bond hydrolysis, exonucleolytic nucleic acid phosphodiester bond hydrolysis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DIS3 Polyclonal Antibody

DIS3 Antibody (14689-1-AP) in ICC/IF

Product Details

14689-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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