Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
DKK1 Antibody (PA5-47198) in IHC (F)
Immunohistochemical analysis of DKK1 in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye). Samples were incubated in DKK1 polyclonal antibody (Product # PA5-47198) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Product Details
PA5-47198
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dkk-1 Ser30-His272
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610219
Product Specific Information
In sandwich ELISAs, less than 6% cross-reactivity with recombinant human Dkk-1 and less than 0.1% cross-reactivity with recombinant mouse (rm) Dkk-2, rmDkk-3, and rmDkk-4 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Dickkopf-1; dickkopf-like protein 1; Dickkopf-related protein 1; Dickkopf1; dkk-1; hDkk1
Gene Aliases: Dkk1; mdkk-1
UniProt ID: (Mouse) O54908
Entrez Gene ID: (Mouse) 13380
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis involved in differentiation
endoderm formation
mesoderm formation
hair follicle development
regulation of receptor internalization
multicellular organism development
endoderm development
Wnt signaling pathway
regulation of Wnt signaling pathway
negative regulation of Wnt signaling pathway
embryonic limb morphogenesis
negative regulation of BMP signaling pathway
forebrain development
negative regulation of protein complex assembly
response to retinoic acid
negative regulation of peptidyl-serine phosphorylation
negative regulation of mesodermal cell fate specification
regulation of endodermal cell fate specification
negative regulation of skeletal muscle tissue development
head morphogenesis
face morphogenesis
negative regulation of pathway-restricted SMAD protein phosphorylation
positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
Wnt signaling pathway involved in somitogenesis
extracellular negative regulation of signal transduction
negative regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment
negative regulation of receptor activity
negative regulation of cardiac muscle cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support