Proteintech

DLD Polyclonal Antibody

DLD Antibody in Immunocytochemistry (ICC/IF)

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DLD Antibody (16431-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 16431-1-AP) (DLD antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DLD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DLD Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16431-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DLD Fusion Protein Ag9729 (160-509 aa encoded by BC018696)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: NQVTATKAD GGTQVIDTKN ILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD RVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN F (160-509 aa encoded by BC018696)

Target Information

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Silencing of OGDHL promotes liver cancer metastasis by enhancing hypoxia inducible factor 1 α protein stability.

Cancer science

Dai W,Li Y,Sun W,Ji M,Bao R,Chen J,Xu S,Dai Y,Chen Y,Liu W,Ge C,Sun W,Mo W,Guo C,Xu X

Sat Apr 01 00:00:00 EDT 2023

Bioinformatics-Based Analysis of Key Genes in Steroid-Induced Osteonecrosis of the Femoral Head That Are Associated with Copper Metabolism.

Biomedicines

Qi B,Li C,Cai X,Pu L,Guo M,Tang Z,Bu P,Xu Y

Mon Mar 13 00:00:00 EDT 2023

ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer.

Frontiers in oncology

Zhu X,Chen H,Li H,Ren H,Ye C,Xu K,Liu J,Du F,Zhang Z,Liu Y,Xie X,Wang M,Ma T,Chong W,Shang L,Li L

Tue Apr 04 00:00:00 EDT 2023

Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.

Cell reports

Liu Y,Liu K,Thorne RF,Shi R,Zhang Q,Wu M,Liu L

Tue Feb 28 00:00:00 EST 2023

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Cellular and molecular life sciences : CMLS

Peng MZ,Shao YX,Li XZ,Zhang KD,Cai YN,Lin YT,Jiang MY,Liu ZC,Su XY,Zhang W,Jiang XL,Liu L

Tue Jun 21 00:00:00 EDT 2022

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways.

Redox biology

Chen Y,Zhang T,Zeng S,Xu R,Jin K,Coorey NJ,Wang Y,Wang K,Lee SR,Yam M,Zhu M,Chang A,Fan X,Zhang M,Du J,Gillies MC,Zhu L

Mon Aug 01 00:00:00 EDT 2022

Beneficial effects of aloperine on inflammation and oxidative stress by suppressing necroptosis in lipopolysaccharide-induced acute lung injury mouse model.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Cui YR,Qu F,Zhong WJ,Yang HH,Zeng J,Huang JH,Liu J,Zhang MY,Zhou Y,Guan CX

Wed Jun 01 00:00:00 EDT 2022

Beneficial effects of aloperine on inflammation and oxidative stress by suppressing necroptosis in lipopolysaccharide-induced acute lung injury mouse model.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Cui YR,Qu F,Zhong WJ,Yang HH,Zeng J,Huang JH,Liu J,Zhang MY,Zhou Y,Guan CX

Wed Jun 01 00:00:00 EDT 2022

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Nature communications

Xia J,Zhang J,Wu X,Du W,Zhu Y,Liu X,Liu Z,Meng B,Guo J,Yang Q,Wang Y,Wang Q,Feng X,Xie G,Shen Y,He Y,Xiang J,Wu M,An G,Qiu L,Jia W,Zhou W

Mon Jul 11 00:00:00 EDT 2022

Metabolon formation regulates branched-chain amino acid oxidation and homeostasis.

Nature metabolism

Patrick M,Gu Z,Zhang G,Wynn RM,Kaphle P,Cao H,Vu H,Cai F,Gao X,Zhang Y,Chen M,Ni M,Chuang DT,DeBerardinis RJ,Xu J

Thu Dec 01 00:00:00 EST 2022

Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.

Experimental eye research

Yang J,Li Y,Gao W,Feng Y,He X,Ni H,Song X,Fan J

Wed Jun 01 00:00:00 EDT 2022

Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.

Experimental eye research

Yang J,Li Y,Gao W,Feng Y,He X,Ni H,Song X,Fan J

Wed Jun 01 00:00:00 EDT 2022

Copper induces cell death by targeting lipoylated TCA cycle proteins.

Science (New York, N.Y.)

Tsvetkov P,Coy S,Petrova B,Dreishpoon M,Verma A,Abdusamad M,Rossen J,Joesch-Cohen L,Humeidi R,Spangler RD,Eaton JK,Frenkel E,Kocak M,Corsello SM,Lutsenko S,Kanarek N,Santagata S,Golub TR

Fri Mar 18 00:00:00 EDT 2022

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Journal of hepatology

Dai W,Xu L,Yu X,Zhang G,Guo H,Liu H,Song G,Weng S,Dong L,Zhu J,Liu T,Guo C,Shen X

Fri May 01 00:00:00 EDT 2020

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis.

Nature cell biology

Yang Z,Wang Y,Zhang Y,He X,Zhong CQ,Ni H,Chen X,Liang Y,Wu J,Zhao S,Zhou D,Han J

Thu Feb 01 00:00:00 EST 2018

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Proteomes

Horita H,Law A,Middleton K

Tue May 22 00:00:00 EDT 2018

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Proteomes

Horita H,Law A,Middleton K

Tue May 22 00:00:00 EDT 2018

Riboflavin responsive mitochondrial myopathy is a new phenotype of dihydrolipoamide dehydrogenase deficiency. The chaperon-like effect of vitamin B2.

Mitochondrion

Carrozzo R,Torraco A,Fiermonte G,Martinelli D,Di Nottia M,Rizza T,Vozza A,Verrigni D,Diodato D,Parisi G,Maiorana A,Rizzo C,Pierri CL,Zucano S,Piemonte F,Bertini E,Dionisi-Vici C

Mon Sep 01 00:00:00 EDT 2014

Riboflavin responsive mitochondrial myopathy is a new phenotype of dihydrolipoamide dehydrogenase deficiency. The chaperon-like effect of vitamin B2.

Mitochondrion

Carrozzo R,Torraco A,Fiermonte G,Martinelli D,Di Nottia M,Rizza T,Vozza A,Verrigni D,Diodato D,Parisi G,Maiorana A,Rizzo C,Pierri CL,Zucano S,Piemonte F,Bertini E,Dionisi-Vici C

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: branched chain alpha-keto acid dehydrogenase complex subunit E3; diaphorase; Dihydrolipoamide dehydrogenase; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex); Dihydrolipoyl dehydrogenase, mitochondrial; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; Glycine cleavage system L protein; glycine cleavage system protein L; lipoamide dehydrogenase; lipoamide reductase; lipoyl dehydrogenase; OTTHUMP00000206744; OTTHUMP00000206746; OTTHUMP00000206748; OTTHUMP00000206749; PHE 3

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Gene Aliases: DLD; DLDD; DLDH; E3; GCSL; LAD; PHE3

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UniProt ID: (Human) P09622, (Rat) Q6P6R2, (Mouse) O08749

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Entrez Gene ID: (Human) 1738, (Rat) 298942, (Mouse) 13382

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Function(s)

dihydrolipoyl dehydrogenase activity pyruvate dehydrogenase (NAD+) activity lipoamide binding flavin adenine dinucleotide binding NAD binding mercury (II) reductase activity mercury ion binding NADP binding oxidoreductase activity oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor oxidoreductase

Process(es)

pyruvate metabolic process tricarboxylic acid cycle 2-oxoglutarate metabolic process mitochondrial electron transport, NADH to ubiquinone proteolysis lysine catabolic process gastrulation aging branched-chain amino acid catabolic process lipoate metabolic process regulation of acetyl-CoA biosynthetic process from pyruvate regulation of membrane potential cell redox homeostasis glyoxylate metabolic process sperm capacitation dihydrolipoamide metabolic process mitochondrial acetyl-CoA biosynthetic process from pyruvate acetyl-CoA biosynthetic process from pyruvate detoxification of mercury ion oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DLD Polyclonal Antibody

DLD Antibody (16431-1-AP) in ICC/IF

Product Details

16431-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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